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@@ -15,8 +15,7 @@ Learn more about contributing: [CONTRIBUTING.md](https://github.com/nf-core/rnas
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-[ ] This comment contains a description of changes (with reason).
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-[ ] If you've fixed a bug or added code that should be tested, add tests!
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-[ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/nf-core/rnaseq/tree/master/.github/CONTRIBUTING.md)
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-[ ] If necessary, also make a PR on the nf-core/rnaseq _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository.
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-[ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/nf-core/rnaseq/tree/master/.github/CONTRIBUTING.md)- [] If necessary, also make a PR on the nf-core/rnaseq _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository.
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-[ ] Make sure your code lints (`nf-core lint`).
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-[ ] Ensure the test suite passes (`nextflow run . -profile test,docker --outdir <OUTDIR>`).
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-[ ] Usage Documentation in `docs/usage.md` is updated.
[](https://github.com/nf-core/rnaseq/actions?query=workflow%3A%22nf-core+CI%22)
[](https://doi.org/10.5281/zenodo.XXXXXXX)
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[](https://nf-co.re/rnaseq/results)[](https://doi.org/10.5281/zenodo.XXXXXXX)
[](https://docs.conda.io/en/latest/)
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[](https://www.docker.com/)
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[](https://sylabs.io/docs/)
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[](https://docs.conda.io/en/latest/)
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[](https://www.docker.com/)
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[](https://sylabs.io/docs/)
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[](https://tower.nf/launch?pipeline=https://github.com/nf-core/rnaseq)
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[](https://nfcore.slack.com/channels/rnaseq)
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[](https://twitter.com/nf_core)
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[](https://www.youtube.com/c/nf-core)
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[](https://nfcore.slack.com/channels/rnaseq)[](https://twitter.com/nf_core)[](https://www.youtube.com/c/nf-core)
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## Introduction
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<!-- TODO nf-core: Write a 1-2 sentence summary of what data the pipeline is for and what it does -->
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**nf-core/rnaseq** is a bioinformatics best-practice analysis pipeline for Nextflow RNA-Seq analysis pipeline, part of the nf-core community..
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**nf-core/rnaseq** is a bioinformatics best-practice analysis pipeline for RNA sequencing analysis pipeline for gene/isoform quantification and extensive quality control..
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The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool to run tasks across multiple compute infrastructures in a very portable manner. It uses Docker/Singularity containers making installation trivial and results highly reproducible. The [Nextflow DSL2](https://www.nextflow.io/docs/latest/dsl2.html) implementation of this pipeline uses one container per process which makes it much easier to maintain and update software dependencies. Where possible, these processes have been submitted to and installed from [nf-core/modules](https://github.com/nf-core/modules) in order to make them available to all nf-core pipelines, and to everyone within the Nextflow community!
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<!-- TODO nf-core: Add full-sized test dataset and amend the paragraph below if applicable -->
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On release, automated continuous integration tests run the pipeline on a full-sized dataset on the AWS cloud infrastructure. This ensures that the pipeline runs on AWS, has sensible resource allocation defaults set to run on real-world datasets, and permits the persistent storage of results to benchmark between pipeline releases and other analysis sources.The results obtained from the full-sized test can be viewed on the [nf-core website](https://nf-co.re/rnaseq/results).
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On release, automated continuous integration tests run the pipeline on a full-sized dataset on the AWS cloud infrastructure. This ensures that the pipeline runs on AWS, has sensible resource allocation defaults set to run on real-world datasets, and permits the persistent storage of results to benchmark between pipeline releases and other analysis sources.The results obtained from the full-sized test can be viewed on the [nf-core website](https://nf-co.re/rnaseq/results).
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## Pipeline summary
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3. Download the pipeline and test it on a minimal dataset with a single command:
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```console
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```bash
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nextflow run nf-core/rnaseq -profile test,YOURPROFILE --outdir <OUTDIR>
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```
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<!-- TODO nf-core: Update the example "typical command" below used to run the pipeline -->
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