diff --git a/tests/component/test_stream_writers_do.py b/tests/component/test_stream_writers_do.py
index 37d013c2a..c68740439 100644
--- a/tests/component/test_stream_writers_do.py
+++ b/tests/component/test_stream_writers_do.py
@@ -160,6 +160,19 @@ def di_port0_loopback_task(
     return task
 
 
+@pytest.fixture
+def di_port0_loopback_task_32dio(
+    generate_task: Callable[[], nidaqmx.Task], real_x_series_device_32dio: nidaqmx.system.Device
+) -> nidaqmx.Task:
+    task = generate_task()
+    task.di_channels.add_di_chan(
+        real_x_series_device_32dio.di_ports[0].name,
+        line_grouping=LineGrouping.CHAN_FOR_ALL_LINES,
+    )
+    _start_di_task(task)
+    return task
+
+
 @pytest.fixture
 def di_port1_loopback_task(
     generate_task: Callable[[], nidaqmx.Task], real_x_series_device: nidaqmx.system.Device
@@ -173,6 +186,19 @@ def di_port1_loopback_task(
     return task
 
 
+@pytest.fixture
+def di_port1_loopback_task_32dio(
+    generate_task: Callable[[], nidaqmx.Task], real_x_series_device_32dio: nidaqmx.system.Device
+) -> nidaqmx.Task:
+    task = generate_task()
+    task.di_channels.add_di_chan(
+        real_x_series_device_32dio.di_ports[1].name,
+        line_grouping=LineGrouping.CHAN_FOR_ALL_LINES,
+    )
+    _start_di_task(task)
+    return task
+
+
 @pytest.fixture
 def di_port2_loopback_task(
     generate_task: Callable[[], nidaqmx.Task], real_x_series_device: nidaqmx.system.Device
@@ -186,6 +212,19 @@ def di_port2_loopback_task(
     return task
 
 
+@pytest.fixture
+def di_port2_loopback_task_32dio(
+    generate_task: Callable[[], nidaqmx.Task], real_x_series_device_32dio: nidaqmx.system.Device
+) -> nidaqmx.Task:
+    task = generate_task()
+    task.di_channels.add_di_chan(
+        real_x_series_device_32dio.di_ports[2].name,
+        line_grouping=LineGrouping.CHAN_FOR_ALL_LINES,
+    )
+    _start_di_task(task)
+    return task
+
+
 @pytest.fixture
 def di_multi_channel_port_loopback_task(
     generate_task: Callable[[], nidaqmx.Task], real_x_series_device: nidaqmx.system.Device
@@ -447,14 +486,14 @@ def test___digital_multi_channel_writer___write_one_sample_multi_line___updates_
 
 
 def test___digital_multi_channel_writer___write_one_sample_multi_line_jagged___updates_output(
-    di_port0_loopback_task: nidaqmx.Task,
-    di_port1_loopback_task: nidaqmx.Task,
-    di_port2_loopback_task: nidaqmx.Task,
+    di_port0_loopback_task_32dio: nidaqmx.Task,
+    di_port1_loopback_task_32dio: nidaqmx.Task,
+    di_port2_loopback_task_32dio: nidaqmx.Task,
     generate_task: Callable[[], nidaqmx.Task],
-    real_x_series_device: nidaqmx.system.Device,
+    real_x_series_device_32dio: nidaqmx.system.Device,
 ) -> None:
     task = generate_task()
-    for port in real_x_series_device.do_ports:
+    for port in real_x_series_device_32dio.do_ports:
         task.do_channels.add_do_chan(
             port.name,
             line_grouping=LineGrouping.CHAN_FOR_ALL_LINES,
@@ -469,9 +508,9 @@ def test___digital_multi_channel_writer___write_one_sample_multi_line_jagged___u
         data_to_write = _int_to_bool_array(num_channels * 32, datum).reshape((num_channels, 32))
         writer.write_one_sample_multi_line(data_to_write)
 
-    assert di_port0_loopback_task.read() == datum & 0xFFFFFFFF
-    assert di_port1_loopback_task.read() == (datum >> 32) & 0xFF
-    assert di_port2_loopback_task.read() == (datum >> 64) & 0xFF
+    assert di_port0_loopback_task_32dio.read() == datum & 0xFFFFFFFF
+    assert di_port1_loopback_task_32dio.read() == (datum >> 32) & 0xFF
+    assert di_port2_loopback_task_32dio.read() == (datum >> 64) & 0xFF
 
 
 def test___digital_multi_channel_writer___write_one_sample_multi_line_with_wrong_dtype___raises_error_with_correct_dtype(
diff --git a/tests/conftest.py b/tests/conftest.py
index d295d9328..8162cd26f 100644
--- a/tests/conftest.py
+++ b/tests/conftest.py
@@ -104,6 +104,7 @@ def _x_series_device(
     device_type: DeviceType,
     system: nidaqmx.system.System,
     sampling_type: SamplingType = SamplingType.ANY,
+    min_num_lines: int = 8,
 ) -> nidaqmx.system.Device:
     for device in system.devices:
         device_type_match = (
@@ -127,7 +128,7 @@ def _x_series_device(
             and device.product_category == ProductCategory.X_SERIES_DAQ
             and len(device.ao_physical_chans) >= 2
             and len(device.ai_physical_chans) >= 4
-            and len(device.do_lines) >= 8
+            and len(device.do_lines) >= min_num_lines
             and len(device.di_lines) == len(device.do_lines)
             and len(device.ci_physical_chans) >= 4
         ):
@@ -180,6 +181,14 @@ def real_x_series_device(system: nidaqmx.system.System) -> nidaqmx.system.Device
     return _x_series_device(DeviceType.REAL, system)
 
 
+@pytest.fixture(scope="function")
+def real_x_series_device_32dio(system: nidaqmx.system.System) -> nidaqmx.system.Device:
+    """Gets real 32 DIO X Series device information."""
+    return _x_series_device(
+        DeviceType.REAL, system, sampling_type=SamplingType.ANY, min_num_lines=32
+    )
+
+
 @pytest.fixture(scope="function")
 def real_x_series_multiplexed_device(system: nidaqmx.system.System) -> nidaqmx.system.Device:
     """Gets device information for a real X Series device with multiplexed sampling."""