diff --git a/tests/drivers/gpio/gpio_nrf/README.txt b/tests/drivers/gpio/gpio_nrf/README.txt
index 6a5927b725f..c73b5ec2218 100644
--- a/tests/drivers/gpio/gpio_nrf/README.txt
+++ b/tests/drivers/gpio/gpio_nrf/README.txt
@@ -2,3 +2,7 @@ Nordic Semiconductor specific GPIO functions
 ############################################
 The suite specified here tests NRF specific
 GPIO pin drive strength settings.
+
+The suite is perfomed with CONFIG_GPIO_NRFX_INTERRUPT=n
+to ensure that the driver is able to properly handle
+the GPIO manipulation without interrupt support.
diff --git a/tests/drivers/gpio/gpio_nrf/prj.conf b/tests/drivers/gpio/gpio_nrf/prj.conf
index 0e799280f51..98d49c26369 100644
--- a/tests/drivers/gpio/gpio_nrf/prj.conf
+++ b/tests/drivers/gpio/gpio_nrf/prj.conf
@@ -1,2 +1,3 @@
 CONFIG_ZTEST=y
 CONFIG_GPIO=y
+CONFIG_GPIO_NRFX_INTERRUPT=n
diff --git a/tests/drivers/gpio/gpio_nrf/src/main.c b/tests/drivers/gpio/gpio_nrf/src/main.c
index 8627e9bab1d..bdb8444bbeb 100644
--- a/tests/drivers/gpio/gpio_nrf/src/main.c
+++ b/tests/drivers/gpio/gpio_nrf/src/main.c
@@ -58,4 +58,38 @@ ZTEST(gpio_nrf, test_gpio_high_drive_strength)
 		err);
 }
 
+/*
+ * Nordic Semiconductor specific,
+ * gpio manipulation with disabled NRFX interrupts
+ */
+ZTEST(gpio_nrf, test_gpio_manipulation_nrfx_int_disabled)
+{
+	int response;
+	const struct device *port;
+
+	port = DEVICE_DT_GET(TEST_NODE);
+	zassert_true(device_is_ready(port), "GPIO dev is not ready");
+
+	response = gpio_pin_configure(port, TEST_PIN, GPIO_OUTPUT | GPIO_ACTIVE_HIGH);
+	zassert_ok(response, "Pin configuration failed: %d", response);
+
+	response = gpio_pin_set(port, TEST_PIN, 0);
+	zassert_ok(response, "Pin low state set failed: %d", response);
+
+	response = gpio_pin_set(port, TEST_PIN, 1);
+	zassert_ok(response, "Pin high state set failed: %d", response);
+
+	response = gpio_pin_toggle(port, TEST_PIN);
+	zassert_ok(response, "Pin toggle failed: %d", response);
+
+	response = gpio_pin_configure(port, TEST_PIN, GPIO_INPUT | GPIO_PULL_DOWN);
+	zassert_ok(response, "Failed to configure pin as input with pull down: %d", response);
+
+	response = gpio_pin_get(port, TEST_PIN);
+	zassert_equal(response, 0, "Invalid pin state: %d", response);
+
+	response = gpio_pin_interrupt_configure(port, TEST_PIN, GPIO_INT_ENABLE | GPIO_INT_HIGH_1);
+	zassert_equal(response, -ENOSYS);
+}
+
 ZTEST_SUITE(gpio_nrf, NULL, NULL, NULL, NULL, NULL);