diff --git a/boards/native/nrf_bsim/nrf54l15bsim_nrf54l15_cpuapp.dts b/boards/native/nrf_bsim/nrf54l15bsim_nrf54l15_cpuapp.dts
index 13bf2e338f81..9a2d3e25c978 100644
--- a/boards/native/nrf_bsim/nrf54l15bsim_nrf54l15_cpuapp.dts
+++ b/boards/native/nrf_bsim/nrf54l15bsim_nrf54l15_cpuapp.dts
@@ -28,7 +28,6 @@
 		/delete-node/ memory@20000000;
 		/delete-node/ memory@2002f000;
 		peripheral@50000000 {
-			/delete-node/ spi@4a000;
 			/delete-node/ vpr@4c000;
 			/delete-node/ mailbox@0;
 			/delete-node/ interrupt-controller@f0000000;
@@ -84,8 +83,8 @@
 	};
 };
 
-&uart00 {
-	/delete-property/ clocks;
+&spi00 {
+	status = "disabled";
 };
 
 &uart20 {
diff --git a/tests/bsim/ci.uart.sh b/tests/bsim/ci.uart.sh
index 70d296d23dfa..7f53fa864065 100755
--- a/tests/bsim/ci.uart.sh
+++ b/tests/bsim/ci.uart.sh
@@ -20,7 +20,7 @@ ${ZEPHYR_BASE}/scripts/twister -T tests/drivers/uart/ --force-color --inline-log
 echo " nRF54L15:"
 ${ZEPHYR_BASE}/scripts/twister -T tests/drivers/uart/ --force-color --inline-logs -v -M \
   -p nrf54l15bsim/nrf54l15/cpuapp --fixture gpio_loopback \
-  -- -uart2_loopback
+  -- -uart0_loopback -uart2_loopback
 
 echo "UART: Multi device tests"
 WORK_DIR=${ZEPHYR_BASE}/bsim_uart nice tests/bsim/drivers/uart/compile.sh
diff --git a/tests/drivers/uart/uart_async_api/boards/nrf54l15dk_nrf54l15_cpuapp.overlay b/tests/drivers/uart/uart_async_api/boards/nrf54l15dk_nrf54l15_cpuapp.overlay
index ed4a2de1891c..a1e29cbf0ffc 100644
--- a/tests/drivers/uart/uart_async_api/boards/nrf54l15dk_nrf54l15_cpuapp.overlay
+++ b/tests/drivers/uart/uart_async_api/boards/nrf54l15dk_nrf54l15_cpuapp.overlay
@@ -1,5 +1,9 @@
 /* SPDX-License-Identifier: Apache-2.0 */
 
+&spi00 {
+	status = "disabled";
+};
+
 &pinctrl {
 	uart21_default_alt: uart21_default_alt {
 		group1 {
@@ -15,6 +19,21 @@
 			low-power-enable;
 		};
 	};
+
+	uart00_default_alt: uart00_default_alt {
+		group1 {
+			psels = <NRF_PSEL(UART_TX, 2, 8)>,
+				<NRF_PSEL(UART_RX, 2, 7)>;
+		};
+	};
+
+	uart00_sleep_alt: uart00_sleep_alt {
+		group1 {
+			psels = <NRF_PSEL(UART_TX, 2, 8)>,
+				<NRF_PSEL(UART_RX, 2, 7)>;
+			low-power-enable;
+		};
+	};
 };
 
 dut: &uart21 {
@@ -24,3 +43,11 @@ dut: &uart21 {
 	pinctrl-names = "default", "sleep";
 	current-speed = <115200>;
 };
+
+dut2: &uart00 {
+	status = "okay";
+	pinctrl-0 = <&uart00_default_alt>;
+	pinctrl-1 = <&uart00_sleep_alt>;
+	pinctrl-names = "default", "sleep";
+	current-speed = <4000000>;
+};