diff --git a/drivers/adc/adc_nrfx_saadc.c b/drivers/adc/adc_nrfx_saadc.c
index 22a839a5f35..5692f910cbb 100644
--- a/drivers/adc/adc_nrfx_saadc.c
+++ b/drivers/adc/adc_nrfx_saadc.c
@@ -719,6 +719,29 @@ static DEVICE_API(adc, adc_nrfx_driver_api) = {
 #endif
 };
 
+#ifdef NRF_SAADC_AIN8
+/* AIN8-AIN14 inputs are on 3v3 GPIO port and they cannot be mixed with other
+ * analog inputs (from 1v8 ports) in differential mode.
+ */
+#define CH_IS_3V3(val) (val >= NRF_SAADC_AIN8)
+
+#define CH_IS_DIFF(node) DT_PROP(node, zephyr_differential)
+
+#define CH_MIX_3V3_1V8_CHECK(node)					\
+	(!CH_IS_DIFF(node) ||						\
+	 !(CH_IS_3V3(DT_PROP_OR(node, zephyr_input_positive, 0)) ^	\
+	   CH_IS_3V3(DT_PROP_OR(node, zephyr_input_negative, 0))))
+#else
+#define CH_MIX_3V3_1V8_CHECK(node) true
+#endif
+
+#define VALIDATE_CHANNEL_CONFIG(node)					\
+	BUILD_ASSERT(CH_MIX_3V3_1V8_CHECK(node) == true,		\
+		     "1v8 inputs cannot be mixed with 3v3 inputs");
+
+/* Validate configuration of all channels. */
+#define VALIDATE_CHANNELS_CONFIG(inst) DT_FOREACH_CHILD(DT_DRV_INST(inst), VALIDATE_CHANNEL_CONFIG)
+
 /*
  * There is only one instance on supported SoCs, so inst is guaranteed
  * to be 0 if any instance is okay. (We use adc_0 above, so the driver
@@ -731,6 +754,7 @@ static DEVICE_API(adc, adc_nrfx_driver_api) = {
 #define SAADC_INIT(inst)						\
 	BUILD_ASSERT((inst) == 0,					\
 		     "multiple instances not supported");		\
+	VALIDATE_CHANNELS_CONFIG(inst)					\
 	PM_DEVICE_DT_INST_DEFINE(0, saadc_pm_hook, 1);			\
 	DEVICE_DT_INST_DEFINE(0,					\
 			    init_saadc,					\
diff --git a/samples/drivers/adc/adc_sequence/boards/nrf54h20dk_nrf54h20_cpuapp.overlay b/samples/drivers/adc/adc_sequence/boards/nrf54h20dk_nrf54h20_cpuapp.overlay
index bf53616d928..24cce679375 100644
--- a/samples/drivers/adc/adc_sequence/boards/nrf54h20dk_nrf54h20_cpuapp.overlay
+++ b/samples/drivers/adc/adc_sequence/boards/nrf54h20dk_nrf54h20_cpuapp.overlay
@@ -45,6 +45,7 @@
 		zephyr,reference = "ADC_REF_INTERNAL";
 		zephyr,acquisition-time = <ADC_ACQ_TIME_DEFAULT>;
 		zephyr,input-positive = <NRF_SAADC_AIN9>; /* P9.01 */
+		zephyr,vref-mv = <3686>; /* 3.6V * 1024 */
 		zephyr,resolution = <12>;
 		zephyr,oversampling = <8>;
 	};
diff --git a/samples/drivers/adc/adc_sequence/src/main.c b/samples/drivers/adc/adc_sequence/src/main.c
index 60bde60650d..084dbf428f3 100644
--- a/samples/drivers/adc/adc_sequence/src/main.c
+++ b/samples/drivers/adc/adc_sequence/src/main.c
@@ -60,7 +60,7 @@ int main(void)
 			printf("Could not setup channel #%d (%d)\n", i, err);
 			return 0;
 		}
-		if (channel_cfgs[i].reference == ADC_REF_INTERNAL) {
+		if ((vrefs_mv[i] == 0) && (channel_cfgs[i].reference == ADC_REF_INTERNAL)) {
 			vrefs_mv[i] = adc_ref_internal(adc);
 		}
 	}