diff --git a/tests/boards/nrf/i2c/i2c_slave/src/main.c b/tests/boards/nrf/i2c/i2c_slave/src/main.c
index 1de4b16bc2c..403c8568290 100644
--- a/tests/boards/nrf/i2c/i2c_slave/src/main.c
+++ b/tests/boards/nrf/i2c/i2c_slave/src/main.c
@@ -21,8 +21,14 @@
 #define I2C_S_INSTANCE 1
 #elif CONFIG_NRFX_TWIS2
 #define I2C_S_INSTANCE 2
+#elif CONFIG_NRFX_TWIS20
+#define I2C_S_INSTANCE 20
+#elif CONFIG_NRFX_TWIS21
+#define I2C_S_INSTANCE 21
 #elif CONFIG_NRFX_TWIS22
 #define I2C_S_INSTANCE 22
+#elif CONFIG_NRFX_TWIS30
+#define I2C_S_INSTANCE 30
 #elif CONFIG_NRFX_TWIS131
 #define I2C_S_INSTANCE 131
 #else