diff --git a/tests/drivers/i2s/i2s_additional/Kconfig b/tests/drivers/i2s/i2s_additional/Kconfig
index 3c80dba4853..ee903546708 100644
--- a/tests/drivers/i2s/i2s_additional/Kconfig
+++ b/tests/drivers/i2s/i2s_additional/Kconfig
@@ -75,6 +75,14 @@ config I2S_TEST_EIGHT_CHANNELS_UNSUPPORTED
 	  When set to 'y', test will check that i2s_configure() returns -EINVAL.
 	  When set to 'n', test will do the transmission.
 
+config I2S_TEST_EIGHT_CHANNELS_32B_48K_UNSUPPORTED
+	bool "Skip test with 8 channels, 32 bit word size and 48k sample rate"
+	depends on !I2S_TEST_EIGHT_CHANNELS_UNSUPPORTED
+	help
+	  Skip test that is challenging due to high data throughput.
+	  When set to 'y', test will be skipped.
+	  When set to 'n', test will do the transmission.
+
 config I2S_TEST_DATA_FORMAT_I2S_UNSUPPORTED
 	bool "I2S_FMT_DATA_FORMAT_I2S is not supported by the driver"
 	help
diff --git a/tests/drivers/i2s/i2s_additional/boards/nrf54h20dk_nrf54h20_cpuapp_aclk.overlay b/tests/drivers/i2s/i2s_additional/boards/nrf54h20dk_nrf54h20_cpuapp_aclk.overlay
new file mode 100644
index 00000000000..ad9e06d0793
--- /dev/null
+++ b/tests/drivers/i2s/i2s_additional/boards/nrf54h20dk_nrf54h20_cpuapp_aclk.overlay
@@ -0,0 +1,16 @@
+/*
+ * Copyright (c) 2025 Nordic Semiconductor ASA
+ *
+ * SPDX-License-Identifier: Apache-2.0
+ */
+
+&audiopll {
+	status = "okay";
+	frequency = <NRFS_AUDIOPLL_FREQ_AUDIO_48K>;
+};
+
+&tdm130 {
+	mck-frequency = <12288000>;
+	mck-clock-source = "ACLK";
+	sck-clock-source = "ACLK";
+};
diff --git a/tests/drivers/i2s/i2s_additional/src/main.c b/tests/drivers/i2s/i2s_additional/src/main.c
index e8852850cee..0dcfc052bdf 100644
--- a/tests/drivers/i2s/i2s_additional/src/main.c
+++ b/tests/drivers/i2s/i2s_additional/src/main.c
@@ -19,7 +19,7 @@ LOG_MODULE_REGISTER(i2s_add, LOG_LEVEL_INF);
 #define NUMBER_OF_CHANNELS 2
 #define FRAME_CLK_FREQ 44100
 
-#define NUM_BLOCKS 20
+#define NUM_BLOCKS 4
 #define TIMEOUT 1000
 
 #define SAMPLES_COUNT 64
@@ -519,9 +519,9 @@ ZTEST(i2s_additional, test_02b_four_channels)
 #endif /* CONFIG_I2S_TEST_FOUR_CHANNELS_UNSUPPORTED */
 }
 
-/** @brief Test I2S transfer with eight channels.
+/** @brief Test I2S transfer with eight channels, 16 bit and 44.1 kHz.
  */
-ZTEST(i2s_additional, test_02c_eight_channels)
+ZTEST(i2s_additional, test_02c_eight_channels_default)
 {
 	struct i2s_config i2s_cfg = default_i2s_cfg;
 
@@ -549,6 +549,43 @@ ZTEST(i2s_additional, test_02c_eight_channels)
 #endif /* CONFIG_I2S_TEST_EIGHT_CHANNELS_UNSUPPORTED */
 }
 
+/** @brief Test I2S transfer with eight channels, 32 bit and 48 kHz.
+ */
+ZTEST(i2s_additional, test_02d_eight_channels_high_throughput)
+{
+	struct i2s_config i2s_cfg = default_i2s_cfg;
+
+	i2s_cfg.channels = 8;
+	i2s_cfg.word_size = 32;
+	i2s_cfg.frame_clk_freq = 48000;
+
+#if defined(CONFIG_I2S_TEST_EIGHT_CHANNELS_UNSUPPORTED)
+	int ret;
+
+	ret = i2s_configure(dev_i2s, I2S_DIR_TX, &i2s_cfg);
+	zassert_equal(ret, -EINVAL, "Unexpected result %d", ret);
+#else /* CONFIG_I2S_TEST_EIGHT_CHANNELS_UNSUPPORTED */
+
+#if defined(CONFIG_I2S_TEST_EIGHT_CHANNELS_32B_48K_UNSUPPORTED)
+	/* Skip this test if driver supports 8ch but fails in this configuration. */
+	ztest_test_skip();
+#endif
+
+	/* Select format that supports eight channels. */
+#if !defined(CONFIG_I2S_TEST_DATA_FORMAT_PCM_LONG_UNSUPPORTED)
+	i2s_cfg.format = I2S_FMT_DATA_FORMAT_PCM_LONG;
+	TC_PRINT("Selected format is I2S_FMT_DATA_FORMAT_PCM_LONG\n");
+#elif !defined(CONFIG_I2S_TEST_DATA_FORMAT_PCM_SHORT_UNSUPPORTED)
+	i2s_cfg.format = I2S_FMT_DATA_FORMAT_PCM_SHORT;
+	TC_PRINT("Selected format is I2S_FMT_DATA_FORMAT_PCM_SHORT\n");
+#else
+#error "Don't know what format supports eight channels."
+#endif
+
+	i2s_dir_both_transfer_long(&i2s_cfg);
+#endif /* CONFIG_I2S_TEST_EIGHT_CHANNELS_UNSUPPORTED */
+}
+
 /** @brief Test I2S transfer with format I2S_FMT_DATA_FORMAT_I2S
  */
 ZTEST(i2s_additional, test_03a_format_i2s)
diff --git a/tests/drivers/i2s/i2s_additional/testcase.yaml b/tests/drivers/i2s/i2s_additional/testcase.yaml
index 9dcb82d61f3..77a18c3ab9d 100644
--- a/tests/drivers/i2s/i2s_additional/testcase.yaml
+++ b/tests/drivers/i2s/i2s_additional/testcase.yaml
@@ -26,6 +26,17 @@ tests:
   drivers.i2s.additional.gpio_loopback.54h:
     harness_config:
       fixture: i2s_loopback
+    extra_configs:
+      - CONFIG_I2S_TEST_EIGHT_CHANNELS_32B_48K_UNSUPPORTED=y
+    platform_allow:
+      - nrf54h20dk/nrf54h20/cpuapp
+    integration_platforms:
+      - nrf54h20dk/nrf54h20/cpuapp
+
+  drivers.i2s.additional.gpio_loopback.54h.aclk:
+    harness_config:
+      fixture: i2s_loopback
+    extra_args: EXTRA_DTC_OVERLAY_FILE="boards/nrf54h20dk_nrf54h20_cpuapp_aclk.overlay"
     platform_allow:
       - nrf54h20dk/nrf54h20/cpuapp
     integration_platforms: