diff --git a/tests/drivers/i2c/i2c_nrfx_twim/boards/nrf54lm20dk_nrf54lm20a_cpuapp.overlay b/tests/drivers/i2c/i2c_nrfx_twim/boards/nrf54lm20dk_nrf54lm20a_cpuapp.overlay
new file mode 100644
index 000000000000..1fc56cc63298
--- /dev/null
+++ b/tests/drivers/i2c/i2c_nrfx_twim/boards/nrf54lm20dk_nrf54lm20a_cpuapp.overlay
@@ -0,0 +1,67 @@
+/*
+ * Copyright (c) 2025 Nordic Semiconductor ASA
+ *
+ * SPDX-License-Identifier: Apache-2.0
+ */
+
+/*
+ * SDA = P1.13 and P1.14
+ * SCL = P1.23 and P1.24
+ */
+
+/ {
+	aliases {
+		i2c-controller = &i2c21;
+		i2c-controller-target = &i2c22;
+	};
+};
+
+&pinctrl {
+	i2c21_default: i2c21_default {
+		group1 {
+			psels = <NRF_PSEL(TWIS_SDA, 1, 13)>,
+				<NRF_PSEL(TWIS_SCL, 1, 23)>;
+			bias-pull-up;
+		};
+	};
+
+	i2c21_sleep: i2c21_sleep {
+		group1 {
+			psels = <NRF_PSEL(TWIS_SDA, 1, 13)>,
+				<NRF_PSEL(TWIS_SCL, 1, 23)>;
+			low-power-enable;
+		};
+	};
+
+	i2c22_default: i2c22_default {
+		group1 {
+			psels = <NRF_PSEL(TWIS_SDA, 1, 14)>,
+				<NRF_PSEL(TWIS_SCL, 1, 24)>;
+			bias-pull-up;
+		};
+	};
+
+	i2c22_sleep: i2c22_sleep {
+		group1 {
+			psels = <NRF_PSEL(TWIS_SDA, 1, 14)>,
+				<NRF_PSEL(TWIS_SCL, 1, 24)>;
+			low-power-enable;
+		};
+	};
+};
+
+&i2c21 {
+	pinctrl-0 = <&i2c21_default>;
+	pinctrl-1 = <&i2c21_sleep>;
+	pinctrl-names = "default", "sleep";
+	zephyr,concat-buf-size = <256>;
+	status = "okay";
+};
+
+&i2c22 {
+	compatible = "nordic,nrf-twis";
+	pinctrl-0 = <&i2c22_default>;
+	pinctrl-1 = <&i2c22_sleep>;
+	pinctrl-names = "default", "sleep";
+	status = "okay";
+};
diff --git a/tests/drivers/i2c/i2c_nrfx_twim/testcase.yaml b/tests/drivers/i2c/i2c_nrfx_twim/testcase.yaml
index 4fa30bae7e84..42d1d98f3af8 100644
--- a/tests/drivers/i2c/i2c_nrfx_twim/testcase.yaml
+++ b/tests/drivers/i2c/i2c_nrfx_twim/testcase.yaml
@@ -14,6 +14,7 @@ tests:
       - nrf54h20dk/nrf54h20/cpuapp
       - nrf54h20dk/nrf54h20/cpuppr
       - nrf54l15dk/nrf54l15/cpuapp
+      - nrf54lm20dk/nrf54lm20a/cpuapp
     integration_platforms:
       - nrf5340dk/nrf5340/cpuapp
       - nrf54l15dk/nrf54l15/cpuapp