diff --git a/src/OpenTelemetry.AutoInstrumentation/Configurations/FileBasedConfiguration/DetectionDevelopment.cs b/src/OpenTelemetry.AutoInstrumentation/Configurations/FileBasedConfiguration/DetectionDevelopment.cs
new file mode 100644
index 0000000000..0db7b77b1e
--- /dev/null
+++ b/src/OpenTelemetry.AutoInstrumentation/Configurations/FileBasedConfiguration/DetectionDevelopment.cs
@@ -0,0 +1,16 @@
+// Copyright The OpenTelemetry Authors
+// SPDX-License-Identifier: Apache-2.0
+
+using Vendors.YamlDotNet.Serialization;
+
+namespace OpenTelemetry.AutoInstrumentation.Configurations.FileBasedConfiguration;
+
+internal class DetectionDevelopment
+{
+    /// <summary>
+    /// Gets or sets the configuration for resource detectors.
+    /// If omitted or null, no resource detectors are enabled.
+    /// </summary>
+    [YamlMember(Alias = "detectors")]
+    public DotNetDetectors? Detectors { get; set; }
+}
diff --git a/src/OpenTelemetry.AutoInstrumentation/Configurations/FileBasedConfiguration/DotNetDetectors.cs b/src/OpenTelemetry.AutoInstrumentation/Configurations/FileBasedConfiguration/DotNetDetectors.cs
new file mode 100644
index 0000000000..4142eedb04
--- /dev/null
+++ b/src/OpenTelemetry.AutoInstrumentation/Configurations/FileBasedConfiguration/DotNetDetectors.cs
@@ -0,0 +1,71 @@
+// Copyright The OpenTelemetry Authors
+// SPDX-License-Identifier: Apache-2.0
+
+using System.Reflection;
+using Vendors.YamlDotNet.Serialization;
+
+namespace OpenTelemetry.AutoInstrumentation.Configurations.FileBasedConfiguration;
+
+internal class DotNetDetectors
+{
+    /// <summary>
+    /// Gets or sets the Azure App Service detector configuration.
+    /// </summary>
+    [YamlMember(Alias = "azureappservice")]
+    public object? AzureAppService { get; set; }
+
+#if NET
+    /// <summary>
+    /// Gets or sets the container detector configuration.
+    /// </summary>
+    [YamlMember(Alias = "container")]
+    public object? Container { get; set; }
+#endif
+
+    /// <summary>
+    /// Gets or sets the host detector configuration.
+    /// </summary>
+    [YamlMember(Alias = "host")]
+    public object? Host { get; set; }
+
+    /// <summary>
+    /// Gets or sets the operating system detector configuration.
+    /// </summary>
+    [YamlMember(Alias = "operatingsystem")]
+    public object? OperatingSystem { get; set; }
+
+    /// <summary>
+    /// Gets or sets the process detector configuration.
+    /// </summary>
+    [YamlMember(Alias = "process")]
+    public object? Process { get; set; }
+
+    /// <summary>
+    /// Gets or sets the process runtime detector configuration.
+    /// </summary>
+    [YamlMember(Alias = "processruntime")]
+    public object? ProcessRuntime { get; set; }
+
+    /// <summary>
+    /// Returns the list of enabled resource detectors.
+    /// </summary>
+    public IReadOnlyList<ResourceDetector> GetEnabledResourceDetectors()
+    {
+        var enabled = new List<ResourceDetector>();
+        var properties = typeof(DotNetDetectors).GetProperties(BindingFlags.Instance | BindingFlags.Public);
+
+        foreach (var prop in properties)
+        {
+            var value = prop.GetValue(this);
+            if (value != null)
+            {
+                if (Enum.TryParse<ResourceDetector>(prop.Name, out var resourceDetector))
+                {
+                    enabled.Add(resourceDetector);
+                }
+            }
+        }
+
+        return enabled;
+    }
+}
diff --git a/src/OpenTelemetry.AutoInstrumentation/Configurations/FileBasedConfiguration/ResourceConfiguration.cs b/src/OpenTelemetry.AutoInstrumentation/Configurations/FileBasedConfiguration/ResourceConfiguration.cs
index 886c906ced..4f5b57af68 100644
--- a/src/OpenTelemetry.AutoInstrumentation/Configurations/FileBasedConfiguration/ResourceConfiguration.cs
+++ b/src/OpenTelemetry.AutoInstrumentation/Configurations/FileBasedConfiguration/ResourceConfiguration.cs
@@ -19,6 +19,12 @@ internal class ResourceConfiguration
     [YamlMember(Alias = "attributes_list")]
     public string? AttributesList { get; set; }
 
+    /// <summary>
+    /// Gets or sets the detection development configuration.
+    /// </summary>
+    [YamlMember(Alias = "detection/development")]
+    public DetectionDevelopment? DetectionDevelopment { get; set; }
+
     public List<KeyValuePair<string, object>> ParseAttributes()
     {
         var resourceAttributesWithPriority = new Dictionary<string, object>();
diff --git a/src/OpenTelemetry.AutoInstrumentation/Configurations/ResourceSettings.cs b/src/OpenTelemetry.AutoInstrumentation/Configurations/ResourceSettings.cs
index 1554a16757..49fc39f207 100644
--- a/src/OpenTelemetry.AutoInstrumentation/Configurations/ResourceSettings.cs
+++ b/src/OpenTelemetry.AutoInstrumentation/Configurations/ResourceSettings.cs
@@ -37,6 +37,6 @@ protected override void OnLoadFile(YamlConfiguration configuration)
 
         Resources = configuration.Resource?.ParseAttributes() ?? [];
 
-        // TODO initialize EnabledDetectors from file configuration
+        EnabledDetectors = configuration.Resource?.DetectionDevelopment?.Detectors?.GetEnabledResourceDetectors() ?? [];
     }
 }
diff --git a/test/OpenTelemetry.AutoInstrumentation.Tests/Configurations/FileBased/FileBasedTestHelper.cs b/test/OpenTelemetry.AutoInstrumentation.Tests/Configurations/FileBased/FileBasedTestHelper.cs
new file mode 100644
index 0000000000..793c7d6b29
--- /dev/null
+++ b/test/OpenTelemetry.AutoInstrumentation.Tests/Configurations/FileBased/FileBasedTestHelper.cs
@@ -0,0 +1,32 @@
+// Copyright The OpenTelemetry Authors
+// SPDX-License-Identifier: Apache-2.0
+
+using System.Reflection;
+using Vendors.YamlDotNet.Core;
+using Vendors.YamlDotNet.Serialization;
+using Xunit;
+
+namespace OpenTelemetry.AutoInstrumentation.Tests.Configurations.FileBased;
+
+internal static class FileBasedTestHelper
+{
+    public static void MoveParserToScalar(IParser parser)
+    {
+        parser.MoveNext(); // StreamStart
+        parser.MoveNext(); // DocumentStart
+        parser.MoveNext(); // Scalar
+    }
+
+    public static void AssertAliasPropertyExists<T>(T obj, string alias)
+    {
+        Assert.NotNull(obj);
+
+        var prop = typeof(T).GetProperties()
+                            .FirstOrDefault(p => p.GetCustomAttribute<YamlMemberAttribute>()?.Alias == alias);
+
+        Assert.NotNull(prop);
+
+        var value = prop.GetValue(obj);
+        Assert.NotNull(value);
+    }
+}
diff --git a/test/OpenTelemetry.AutoInstrumentation.Tests/Configurations/FileBased/FilebasedGeneralSettingsTests.cs b/test/OpenTelemetry.AutoInstrumentation.Tests/Configurations/FileBased/FilebasedResourceSettingsTests.cs
similarity index 75%
rename from test/OpenTelemetry.AutoInstrumentation.Tests/Configurations/FileBased/FilebasedGeneralSettingsTests.cs
rename to test/OpenTelemetry.AutoInstrumentation.Tests/Configurations/FileBased/FilebasedResourceSettingsTests.cs
index 52ab2d51be..2e8fcdafa8 100644
--- a/test/OpenTelemetry.AutoInstrumentation.Tests/Configurations/FileBased/FilebasedGeneralSettingsTests.cs
+++ b/test/OpenTelemetry.AutoInstrumentation.Tests/Configurations/FileBased/FilebasedResourceSettingsTests.cs
@@ -7,7 +7,7 @@
 
 namespace OpenTelemetry.AutoInstrumentation.Tests.Configurations.FileBased;
 
-public class FilebasedGeneralSettingsTests
+public class FilebasedResourceSettingsTests
 {
     [Fact]
     public void LoadFile_MergesBaseAndCustomResourceAttributes()
@@ -97,4 +97,40 @@ public void LoadFile_AttributesListOverwrittenByAttributes()
 
         Assert.Equal("fromAttributes", result["key1"]);
     }
+
+    [Fact]
+    public void GetEnabledResourceDetector_ReturnsCorrectEnabledDetectors()
+    {
+        var detectors = new DotNetDetectors
+        {
+            AzureAppService = new object(),
+            Host = new object(),
+            OperatingSystem = null,
+            Process = new object(),
+            ProcessRuntime = null
+        };
+
+#if NET
+        detectors.Container = new object();
+#endif
+
+        var result = detectors.GetEnabledResourceDetectors();
+
+        var expected = new List<ResourceDetector>
+        {
+            ResourceDetector.AzureAppService,
+            ResourceDetector.Host,
+            ResourceDetector.Process
+        };
+
+#if NET
+        expected.Add(ResourceDetector.Container);
+#endif
+
+        Assert.Equal(expected.Count, result.Count);
+        foreach (var detector in expected)
+        {
+            Assert.Contains(detector, result);
+        }
+    }
 }
diff --git a/test/OpenTelemetry.AutoInstrumentation.Tests/Configurations/FileBased/Files/TestFile.yaml b/test/OpenTelemetry.AutoInstrumentation.Tests/Configurations/FileBased/Files/TestFile.yaml
deleted file mode 100644
index a6722a1859..0000000000
--- a/test/OpenTelemetry.AutoInstrumentation.Tests/Configurations/FileBased/Files/TestFile.yaml
+++ /dev/null
@@ -1,7 +0,0 @@
-file_format: "1.0-rc.1"
-
-resource:
-  attributes:
-    - name: service.name
-      value: unknown_service
-  attributes_list:
diff --git a/test/OpenTelemetry.AutoInstrumentation.Tests/Configurations/FileBased/Files/TestResourceFile.yaml b/test/OpenTelemetry.AutoInstrumentation.Tests/Configurations/FileBased/Files/TestResourceFile.yaml
new file mode 100644
index 0000000000..4a6a91c840
--- /dev/null
+++ b/test/OpenTelemetry.AutoInstrumentation.Tests/Configurations/FileBased/Files/TestResourceFile.yaml
@@ -0,0 +1,16 @@
+file_format: "1.0-rc.1"
+
+resource:
+  attributes:
+    - name: service.name
+      value: unknown_service
+  attributes_list:
+  detection/development:
+    detectors:
+      azureappservice:
+      container:
+      host:
+      operatingsystem:
+      process:
+      processruntime:
+
diff --git a/test/OpenTelemetry.AutoInstrumentation.Tests/Configurations/FileBased/Files/TestFileEnvVars.yaml b/test/OpenTelemetry.AutoInstrumentation.Tests/Configurations/FileBased/Files/TestResourceFileEnvVars.yaml
similarity index 100%
rename from test/OpenTelemetry.AutoInstrumentation.Tests/Configurations/FileBased/Files/TestFileEnvVars.yaml
rename to test/OpenTelemetry.AutoInstrumentation.Tests/Configurations/FileBased/Files/TestResourceFileEnvVars.yaml
diff --git a/test/OpenTelemetry.AutoInstrumentation.Tests/Configurations/FileBasedConfiguration/Parser/ConditionalDeserializerTests.cs b/test/OpenTelemetry.AutoInstrumentation.Tests/Configurations/FileBased/Parser/ConditionalDeserializerTests.cs
similarity index 97%
rename from test/OpenTelemetry.AutoInstrumentation.Tests/Configurations/FileBasedConfiguration/Parser/ConditionalDeserializerTests.cs
rename to test/OpenTelemetry.AutoInstrumentation.Tests/Configurations/FileBased/Parser/ConditionalDeserializerTests.cs
index 18feab0c9d..0a4ee5fa4e 100644
--- a/test/OpenTelemetry.AutoInstrumentation.Tests/Configurations/FileBasedConfiguration/Parser/ConditionalDeserializerTests.cs
+++ b/test/OpenTelemetry.AutoInstrumentation.Tests/Configurations/FileBased/Parser/ConditionalDeserializerTests.cs
@@ -2,12 +2,13 @@
 // SPDX-License-Identifier: Apache-2.0
 
 using OpenTelemetry.AutoInstrumentation.Configurations.FileBasedConfiguration.Parser;
+using OpenTelemetry.AutoInstrumentation.Tests.Configurations.FileBased;
 using Vendors.YamlDotNet.Core;
 using Vendors.YamlDotNet.Serialization;
 using Xunit;
 using YamlParser = Vendors.YamlDotNet.Core.Parser;
 
-namespace OpenTelemetry.AutoInstrumentation.Tests.Configurations.FileBasedConfiguration.Parser;
+namespace OpenTelemetry.AutoInstrumentation.Tests.Configurations.FileBased.Parser;
 
 public class ConditionalDeserializerTests
 {
diff --git a/test/OpenTelemetry.AutoInstrumentation.Tests/Configurations/FileBasedConfiguration/Parser/EnvVarTypeConverterTests.cs b/test/OpenTelemetry.AutoInstrumentation.Tests/Configurations/FileBased/Parser/EnvVarTypeConverterTests.cs
similarity index 99%
rename from test/OpenTelemetry.AutoInstrumentation.Tests/Configurations/FileBasedConfiguration/Parser/EnvVarTypeConverterTests.cs
rename to test/OpenTelemetry.AutoInstrumentation.Tests/Configurations/FileBased/Parser/EnvVarTypeConverterTests.cs
index 56bfc5b38f..9fc348687f 100644
--- a/test/OpenTelemetry.AutoInstrumentation.Tests/Configurations/FileBasedConfiguration/Parser/EnvVarTypeConverterTests.cs
+++ b/test/OpenTelemetry.AutoInstrumentation.Tests/Configurations/FileBased/Parser/EnvVarTypeConverterTests.cs
@@ -5,7 +5,7 @@
 using Xunit;
 using YamlParser = Vendors.YamlDotNet.Core.Parser;
 
-namespace OpenTelemetry.AutoInstrumentation.Tests.Configurations.FileBasedConfiguration.Parser;
+namespace OpenTelemetry.AutoInstrumentation.Tests.Configurations.FileBased.Parser;
 
 public class EnvVarTypeConverterTests
 {
diff --git a/test/OpenTelemetry.AutoInstrumentation.Tests/Configurations/FileBased/ParserTests.cs b/test/OpenTelemetry.AutoInstrumentation.Tests/Configurations/FileBased/Parser/ParserResourceTests.cs
similarity index 57%
rename from test/OpenTelemetry.AutoInstrumentation.Tests/Configurations/FileBased/ParserTests.cs
rename to test/OpenTelemetry.AutoInstrumentation.Tests/Configurations/FileBased/Parser/ParserResourceTests.cs
index ddec15a763..98c20cbffb 100644
--- a/test/OpenTelemetry.AutoInstrumentation.Tests/Configurations/FileBased/ParserTests.cs
+++ b/test/OpenTelemetry.AutoInstrumentation.Tests/Configurations/FileBased/Parser/ParserResourceTests.cs
@@ -1,18 +1,18 @@
 // Copyright The OpenTelemetry Authors
 // SPDX-License-Identifier: Apache-2.0
 
-using OpenTelemetry.AutoInstrumentation.Configurations.FileBasedConfiguration.Parser;
 using Xunit;
+using YamlParser = OpenTelemetry.AutoInstrumentation.Configurations.FileBasedConfiguration.Parser.Parser;
 
-namespace OpenTelemetry.AutoInstrumentation.Tests.Configurations.FileBased;
+namespace OpenTelemetry.AutoInstrumentation.Tests.Configurations.FileBased.Parser;
 
 [Collection("Non-Parallel Collection")]
-public class ParserTests
+public class ParserResourceTests
 {
     [Fact]
     public void Parse_FullConfigYaml_ShouldPopulateModelCorrectly()
     {
-        var config = Parser.ParseYaml("Configurations/FileBased/Files/TestFile.yaml");
+        var config = YamlParser.ParseYaml("Configurations/FileBased/Files/TestResourceFile.yaml");
 
         Assert.NotNull(config);
 
@@ -28,6 +28,26 @@ public void Parse_FullConfigYaml_ShouldPopulateModelCorrectly()
 
         Assert.NotNull(config.Resource.AttributesList);
         Assert.Empty(config.Resource.AttributesList);
+        Assert.NotNull(config.Resource.DetectionDevelopment);
+
+#if NET
+        string[] expectedDetecors = [
+            "azureappservice", "container", "host", "operatingsystem", "process", "processruntime"
+                ];
+#endif
+#if NETFRAMEWORK
+        string[] expectedDetecors = [
+            "azureappservice", "host", "operatingsystem", "process", "processruntime"
+                ];
+#endif
+
+        var detectors = config.Resource.DetectionDevelopment.Detectors;
+        Assert.NotNull(detectors);
+
+        foreach (var alias in expectedDetecors)
+        {
+            FileBasedTestHelper.AssertAliasPropertyExists(detectors, alias);
+        }
     }
 
     [Fact]
@@ -37,7 +57,7 @@ public void Parse_EnvVarYaml_ShouldPopulateModelCompletely()
         Environment.SetEnvironmentVariable("OTEL_SERVICE_NAME", "my‑service");
         Environment.SetEnvironmentVariable("OTEL_RESOURCE_ATTRIBUTES", "key=value");
 
-        var config = Parser.ParseYaml("Configurations/FileBased/Files/TestFileEnvVars.yaml");
+        var config = YamlParser.ParseYaml("Configurations/FileBased/Files/TestResourceFileEnvVars.yaml");
 
         Assert.Equal("1.0-rc.1", config.FileFormat);
         var serviceAttr = config.Resource?.Attributes?.First(a => a.Name == "service.name");
diff --git a/test/OpenTelemetry.AutoInstrumentation.Tests/Configurations/FileBasedConfiguration/FileBasedTestHelper.cs b/test/OpenTelemetry.AutoInstrumentation.Tests/Configurations/FileBasedConfiguration/FileBasedTestHelper.cs
deleted file mode 100644
index 5f87f9ceff..0000000000
--- a/test/OpenTelemetry.AutoInstrumentation.Tests/Configurations/FileBasedConfiguration/FileBasedTestHelper.cs
+++ /dev/null
@@ -1,16 +0,0 @@
-// Copyright The OpenTelemetry Authors
-// SPDX-License-Identifier: Apache-2.0
-
-using Vendors.YamlDotNet.Core;
-
-namespace OpenTelemetry.AutoInstrumentation.Tests.Configurations.FileBasedConfiguration;
-
-internal static class FileBasedTestHelper
-{
-    public static void MoveParserToScalar(IParser parser)
-    {
-        parser.MoveNext(); // StreamStart
-        parser.MoveNext(); // DocumentStart
-        parser.MoveNext(); // Scalar
-    }
-}
diff --git a/test/OpenTelemetry.AutoInstrumentation.Tests/OpenTelemetry.AutoInstrumentation.Tests.csproj b/test/OpenTelemetry.AutoInstrumentation.Tests/OpenTelemetry.AutoInstrumentation.Tests.csproj
index 0aa0bc3fb7..50c9bfa3f0 100644
--- a/test/OpenTelemetry.AutoInstrumentation.Tests/OpenTelemetry.AutoInstrumentation.Tests.csproj
+++ b/test/OpenTelemetry.AutoInstrumentation.Tests/OpenTelemetry.AutoInstrumentation.Tests.csproj
@@ -12,10 +12,10 @@
   </ItemGroup>
 
   <ItemGroup>
-    <None Update="Configurations\FileBased\Files\TestFile.yaml">
+    <None Update="Configurations\FileBased\Files\TestResourceFile.yaml">
       <CopyToOutputDirectory>PreserveNewest</CopyToOutputDirectory>
     </None>
-    <None Update="Configurations\FileBased\Files\TestFileEnvVars.yaml">
+    <None Update="Configurations\FileBased\Files\TestResourceFileEnvVars.yaml">
       <CopyToOutputDirectory>PreserveNewest</CopyToOutputDirectory>
     </None>
   </ItemGroup>