diff --git a/plugins/node/opentelemetry-instrumentation-express/src/instrumentation.ts b/plugins/node/opentelemetry-instrumentation-express/src/instrumentation.ts
index 2c64ef79f8..49dba38f69 100644
--- a/plugins/node/opentelemetry-instrumentation-express/src/instrumentation.ts
+++ b/plugins/node/opentelemetry-instrumentation-express/src/instrumentation.ts
@@ -187,7 +187,10 @@ export class ExpressInstrumentation extends InstrumentationBase<ExpressInstrumen
         ] as ExpressLayerType;
 
         const rpcMetadata = getRPCMetadata(context.active());
-        if (rpcMetadata?.type === RPCType.HTTP) {
+        if (
+          rpcMetadata?.type === RPCType.HTTP &&
+          (!rpcMetadata.route || rpcMetadata?.route?.length < route?.length)
+        ) {
           rpcMetadata.route = route || '/';
         }