diff --git a/.github/workflows/e2e.yml b/.github/workflows/e2e.yml
index 69d271f5a6..161ab2aa91 100644
--- a/.github/workflows/e2e.yml
+++ b/.github/workflows/e2e.yml
@@ -20,6 +20,7 @@ jobs:
           - "20"
           - "22"
           - "24"
+          - "25"
     runs-on: ubuntu-latest
     steps:
       - uses: actions/checkout@v5
@@ -44,7 +45,8 @@ jobs:
         if: ${{
           matrix.node_version == '20' ||
           matrix.node_version == '22' ||
-          matrix.node_version == '24'
+          matrix.node_version == '24' ||
+          matrix.node_version == '25'
           }}
 
       - name: Bootstrap
diff --git a/.github/workflows/unit-test.yml b/.github/workflows/unit-test.yml
index 02fd66e5a8..c2d4d15476 100644
--- a/.github/workflows/unit-test.yml
+++ b/.github/workflows/unit-test.yml
@@ -21,6 +21,7 @@ jobs:
           - "20"
           - "22"
           - "24"
+          - "25"
     runs-on: ubuntu-latest
     steps:
       - name: Checkout
@@ -46,7 +47,8 @@ jobs:
         if: ${{
           matrix.node_version == '20' ||
           matrix.node_version == '22' ||
-          matrix.node_version == '24'
+          matrix.node_version == '24' ||
+          matrix.node_version == '25'
           }}
 
       - name: Bootstrap
@@ -56,13 +58,13 @@ jobs:
         run: npm run compile
 
       - run: npm test
-        if: ${{ matrix.node_version != '22' && matrix.node_version != '24' }}
+        if: ${{ matrix.node_version != '22' && matrix.node_version != '24' || matrix.node_version != '25' }}
       # Node.js type stripping conflicts with mocha usage of ts-node.
       # See https://github.com/open-telemetry/opentelemetry-js/issues/5415
       - run: npm test
         env:
           NODE_OPTIONS: '--no-experimental-strip-types'
-        if: ${{ matrix.node_version == '22' || matrix.node_version == '24' }}
+        if: ${{ matrix.node_version == '22' || matrix.node_version == '24' || matrix.node_version == '25' }}
 
       - name: Report Coverage
         uses: codecov/codecov-action@v5
diff --git a/CHANGELOG.md b/CHANGELOG.md
index 54dd67ec33..1bc347b47d 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -33,6 +33,7 @@ For notes on migrating to 2.x / 0.200.x see [the upgrade guide](doc/upgrade-to-2
 * test(exporter-jaeger): clean up OTEL_EXPORTER_JAEGER_AGENT_PORT between tests [#6003](https://github.com/open-telemetry/opentelemetry-js/pull/6003) @cjihrig
 * test(sdk-trace-base): ensure environment variables are cleaned up between tests [#6011](https://github.com/open-telemetry/opentelemetry-js/pull/6011) @cjihrig
 * perf(opentelemetry-core): optimize attribute serialization [#5866](https://github.com/open-telemetry/opentelemetry-js/pull/5866) @43081j
+* test: test Node.js 25 in CI [#6019](https://github.com/open-telemetry/opentelemetry-js/pull/6019) @cjihrig
 
 ## 2.1.0