diff --git a/instrumentation/opentelemetry-instrumentation-aiohttp-client/test-requirements.txt b/instrumentation/opentelemetry-instrumentation-aiohttp-client/test-requirements.txt
index 04f853bdbf..55ddf646dc 100644
--- a/instrumentation/opentelemetry-instrumentation-aiohttp-client/test-requirements.txt
+++ b/instrumentation/opentelemetry-instrumentation-aiohttp-client/test-requirements.txt
@@ -13,7 +13,7 @@ http_server_mock==1.7
 idna==3.7
 iniconfig==2.0.0
 itsdangerous==2.1.2
-Jinja2==3.1.4
+Jinja2==3.1.6
 MarkupSafe==2.1.5
 multidict==6.0.5
 packaging==24.0
diff --git a/instrumentation/opentelemetry-instrumentation-boto/test-requirements.txt b/instrumentation/opentelemetry-instrumentation-boto/test-requirements.txt
index bde6a42031..d73e7447ed 100644
--- a/instrumentation/opentelemetry-instrumentation-boto/test-requirements.txt
+++ b/instrumentation/opentelemetry-instrumentation-boto/test-requirements.txt
@@ -10,7 +10,7 @@ Deprecated==1.2.14
 docker==7.0.0
 idna==3.7
 iniconfig==2.0.0
-Jinja2==3.1.4
+Jinja2==3.1.6
 jmespath==1.0.1
 MarkupSafe==2.1.5
 moto==2.3.2
diff --git a/instrumentation/opentelemetry-instrumentation-botocore/test-requirements-0.txt b/instrumentation/opentelemetry-instrumentation-botocore/test-requirements-0.txt
index ee28a1f2ba..34442c9202 100644
--- a/instrumentation/opentelemetry-instrumentation-botocore/test-requirements-0.txt
+++ b/instrumentation/opentelemetry-instrumentation-botocore/test-requirements-0.txt
@@ -10,7 +10,7 @@ Deprecated==1.2.14
 docker==7.0.0
 idna==3.7
 iniconfig==2.0.0
-Jinja2==3.1.4
+Jinja2==3.1.6
 jmespath==1.0.1
 MarkupSafe==2.1.5
 moto==5.0.9
diff --git a/instrumentation/opentelemetry-instrumentation-botocore/test-requirements-1.txt b/instrumentation/opentelemetry-instrumentation-botocore/test-requirements-1.txt
index c4695ff27c..a2fa776548 100644
--- a/instrumentation/opentelemetry-instrumentation-botocore/test-requirements-1.txt
+++ b/instrumentation/opentelemetry-instrumentation-botocore/test-requirements-1.txt
@@ -10,7 +10,7 @@ Deprecated==1.2.14
 docker==7.0.0
 idna==3.7
 iniconfig==2.0.0
-Jinja2==3.1.4
+Jinja2==3.1.6
 jmespath==1.0.1
 MarkupSafe==2.1.5
 moto==5.0.9
diff --git a/instrumentation/opentelemetry-instrumentation-click/test-requirements.txt b/instrumentation/opentelemetry-instrumentation-click/test-requirements.txt
index e07bd05bb7..f4d715a5e1 100644
--- a/instrumentation/opentelemetry-instrumentation-click/test-requirements.txt
+++ b/instrumentation/opentelemetry-instrumentation-click/test-requirements.txt
@@ -5,7 +5,7 @@ Deprecated==1.2.14
 Flask==3.0.2
 iniconfig==2.0.0
 itsdangerous==2.1.2
-Jinja2==3.1.4
+Jinja2==3.1.6
 MarkupSafe==2.1.2
 packaging==24.0
 pluggy==1.5.0
diff --git a/instrumentation/opentelemetry-instrumentation-flask/test-requirements-0.txt b/instrumentation/opentelemetry-instrumentation-flask/test-requirements-0.txt
index d1c4687d83..6bebbe414a 100644
--- a/instrumentation/opentelemetry-instrumentation-flask/test-requirements-0.txt
+++ b/instrumentation/opentelemetry-instrumentation-flask/test-requirements-0.txt
@@ -4,7 +4,7 @@ Deprecated==1.2.14
 Flask==2.1.3
 iniconfig==2.0.0
 itsdangerous==2.1.2
-Jinja2==3.1.4
+Jinja2==3.1.6
 MarkupSafe==2.1.2
 packaging==24.0
 pluggy==1.5.0
diff --git a/instrumentation/opentelemetry-instrumentation-flask/test-requirements-1.txt b/instrumentation/opentelemetry-instrumentation-flask/test-requirements-1.txt
index 37812de57e..c66c55df1d 100644
--- a/instrumentation/opentelemetry-instrumentation-flask/test-requirements-1.txt
+++ b/instrumentation/opentelemetry-instrumentation-flask/test-requirements-1.txt
@@ -4,7 +4,7 @@ Deprecated==1.2.14
 Flask==2.2.0
 iniconfig==2.0.0
 itsdangerous==2.1.2
-Jinja2==3.1.4
+Jinja2==3.1.6
 MarkupSafe==2.1.2
 packaging==24.0
 pluggy==1.5.0
diff --git a/instrumentation/opentelemetry-instrumentation-flask/test-requirements-2.txt b/instrumentation/opentelemetry-instrumentation-flask/test-requirements-2.txt
index d7bf77e315..3736417419 100644
--- a/instrumentation/opentelemetry-instrumentation-flask/test-requirements-2.txt
+++ b/instrumentation/opentelemetry-instrumentation-flask/test-requirements-2.txt
@@ -5,7 +5,7 @@ Deprecated==1.2.14
 Flask==3.0.2
 iniconfig==2.0.0
 itsdangerous==2.1.2
-Jinja2==3.1.4
+Jinja2==3.1.6
 MarkupSafe==2.1.2
 packaging==24.0
 pluggy==1.5.0
diff --git a/instrumentation/opentelemetry-instrumentation-jinja2/test-requirements.txt b/instrumentation/opentelemetry-instrumentation-jinja2/test-requirements.txt
index ffc92e3ffd..a4f8145b1e 100644
--- a/instrumentation/opentelemetry-instrumentation-jinja2/test-requirements.txt
+++ b/instrumentation/opentelemetry-instrumentation-jinja2/test-requirements.txt
@@ -1,7 +1,7 @@
 asgiref==3.8.1
 Deprecated==1.2.14
 iniconfig==2.0.0
-Jinja2==3.1.4
+Jinja2==3.1.6
 MarkupSafe==2.0.1
 packaging==24.0
 pluggy==1.5.0
diff --git a/instrumentation/opentelemetry-instrumentation-tornado/test-requirements.txt b/instrumentation/opentelemetry-instrumentation-tornado/test-requirements.txt
index 2df7df38ec..d8cba715f2 100644
--- a/instrumentation/opentelemetry-instrumentation-tornado/test-requirements.txt
+++ b/instrumentation/opentelemetry-instrumentation-tornado/test-requirements.txt
@@ -9,7 +9,7 @@ http_server_mock==1.7
 idna==3.7
 iniconfig==2.0.0
 itsdangerous==2.1.2
-Jinja2==3.1.4
+Jinja2==3.1.6
 MarkupSafe==2.1.5
 packaging==24.0
 pluggy==1.5.0