diff --git a/CHANGELOG.md b/CHANGELOG.md
index 056fe9686b..16f54fea2d 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -20,9 +20,10 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
   ([#3423](https://github.com/open-telemetry/opentelemetry-python-contrib/pull/3423))
 - `opentelemetry-instrumentation-asyncio` Fix duplicate instrumentation
   ([#3383](https://github.com/open-telemetry/opentelemetry-python-contrib/issues/3383))
-
 - `opentelemetry-instrumentation-botocore` Add GenAI instrumentation for additional Bedrock models for InvokeModel API
   ([#3419](https://github.com/open-telemetry/opentelemetry-python-contrib/pull/3419))
+- `opentelemetry-instrumentation` don't print duplicated conflict log error message
+  ([#3432](https://github.com/open-telemetry/opentelemetry-python-contrib/pull/3432))
 
 ## Version 1.32.0/0.53b0 (2025-04-10)
 
diff --git a/opentelemetry-instrumentation/src/opentelemetry/instrumentation/instrumentor.py b/opentelemetry-instrumentation/src/opentelemetry/instrumentation/instrumentor.py
index dead45fbac..aee0ba2b68 100644
--- a/opentelemetry-instrumentation/src/opentelemetry/instrumentation/instrumentor.py
+++ b/opentelemetry-instrumentation/src/opentelemetry/instrumentation/instrumentor.py
@@ -111,9 +111,12 @@ def instrument(self, **kwargs: Any):
         if not skip_dep_check:
             conflict = self._check_dependency_conflicts()
             if conflict:
-                _LOG.error(conflict)
+                # auto-instrumentation path: don't log conflict as error, instead
+                # let _load_instrumentors handle the exception
                 if raise_exception_on_conflict:
                     raise DependencyConflictError(conflict)
+                # manual instrumentation path: log the conflict as error
+                _LOG.error(conflict)
                 return None
 
         # initialize semantic conventions opt-in if needed
diff --git a/opentelemetry-instrumentation/tests/test_instrumentor.py b/opentelemetry-instrumentation/tests/test_instrumentor.py
index b7e039e797..f5efd25fc7 100644
--- a/opentelemetry-instrumentation/tests/test_instrumentor.py
+++ b/opentelemetry-instrumentation/tests/test_instrumentor.py
@@ -54,11 +54,12 @@ def test_protect(self):
     def test_singleton(self):
         self.assertIs(self.Instrumentor(), self.Instrumentor())
 
+    @patch("opentelemetry.instrumentation.instrumentor._LOG")
     @patch(
         "opentelemetry.instrumentation.instrumentor.BaseInstrumentor._check_dependency_conflicts"
     )
     def test_instrument_missing_dependency_raise(
-        self, mock__check_dependency_conflicts
+        self, mock__check_dependency_conflicts, mock_logger
     ):
         instrumentor = self.Instrumentor()
 
@@ -71,3 +72,19 @@ def test_instrument_missing_dependency_raise(
             instrumentor.instrument,
             raise_exception_on_conflict=True,
         )
+        mock_logger.error.assert_not_called()
+
+    @patch("opentelemetry.instrumentation.instrumentor._LOG")
+    @patch(
+        "opentelemetry.instrumentation.instrumentor.BaseInstrumentor._check_dependency_conflicts"
+    )
+    def test_instrument_missing_dependency_log_error(
+        self, mock__check_dependency_conflicts, mock_logger
+    ):
+        instrumentor = self.Instrumentor()
+        conflict = DependencyConflict("missing", "missing")
+        mock__check_dependency_conflicts.return_value = conflict
+        self.assertIsNone(
+            instrumentor.instrument(raise_exception_on_conflict=False)
+        )
+        mock_logger.error.assert_any_call(conflict)