diff --git a/CHANGELOG.md b/CHANGELOG.md
index ae22221e3f..efb766bda4 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -11,6 +11,12 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 
 ## Unreleased
 
+### Breaking changes
+
+- `opentelemetry-instrumentation-botocore` Use `cloud.region` instead of `aws.region` span attribute as per semantic conventions.
+  ([#3474](https://github.com/open-telemetry/opentelemetry-python-contrib/pull/3474))
+
+
 ## Version 1.33.0/0.54b0 (2025-05-09)
 
 ### Added
diff --git a/instrumentation/opentelemetry-instrumentation-botocore/src/opentelemetry/instrumentation/botocore/__init__.py b/instrumentation/opentelemetry-instrumentation-botocore/src/opentelemetry/instrumentation/botocore/__init__.py
index 45f50c2b2d..0649d62893 100644
--- a/instrumentation/opentelemetry-instrumentation-botocore/src/opentelemetry/instrumentation/botocore/__init__.py
+++ b/instrumentation/opentelemetry-instrumentation-botocore/src/opentelemetry/instrumentation/botocore/__init__.py
@@ -109,6 +109,9 @@ def response_hook(span, service_name, operation_name, result):
 )
 from opentelemetry.metrics import Instrument, Meter, get_meter
 from opentelemetry.propagators.aws.aws_xray_propagator import AwsXRayPropagator
+from opentelemetry.semconv._incubating.attributes.cloud_attributes import (
+    CLOUD_REGION,
+)
 from opentelemetry.semconv.trace import SpanAttributes
 from opentelemetry.trace import get_tracer
 from opentelemetry.trace.span import Span
@@ -276,8 +279,7 @@ def _patched_api_call(self, original_func, instance, args, kwargs):
             SpanAttributes.RPC_SYSTEM: "aws-api",
             SpanAttributes.RPC_SERVICE: call_context.service_id,
             SpanAttributes.RPC_METHOD: call_context.operation,
-            # TODO: update when semantic conventions exist
-            "aws.region": call_context.region,
+            CLOUD_REGION: call_context.region,
             **get_server_attributes(call_context.endpoint_url),
         }
 
diff --git a/instrumentation/opentelemetry-instrumentation-botocore/tests/test_botocore_instrumentation.py b/instrumentation/opentelemetry-instrumentation-botocore/tests/test_botocore_instrumentation.py
index c9b985e877..6ebb074deb 100644
--- a/instrumentation/opentelemetry-instrumentation-botocore/tests/test_botocore_instrumentation.py
+++ b/instrumentation/opentelemetry-instrumentation-botocore/tests/test_botocore_instrumentation.py
@@ -27,6 +27,9 @@
 )
 from opentelemetry.propagate import get_global_textmap, set_global_textmap
 from opentelemetry.propagators.aws.aws_xray_propagator import TRACE_HEADER_KEY
+from opentelemetry.semconv._incubating.attributes.cloud_attributes import (
+    CLOUD_REGION,
+)
 from opentelemetry.semconv.trace import SpanAttributes
 from opentelemetry.test.mock_textmap import MockTextMapPropagator
 from opentelemetry.test.test_base import TestBase
@@ -61,7 +64,7 @@ def _default_span_attributes(self, service: str, operation: str):
             SpanAttributes.RPC_SYSTEM: "aws-api",
             SpanAttributes.RPC_SERVICE: service,
             SpanAttributes.RPC_METHOD: operation,
-            "aws.region": self.region,
+            CLOUD_REGION: self.region,
             "retry_attempts": 0,
             SpanAttributes.HTTP_STATUS_CODE: 200,
             # Some services like IAM or STS have a global endpoint and exclude specified region.
@@ -527,7 +530,7 @@ def test_server_attributes(self):
             attributes={
                 SpanAttributes.SERVER_ADDRESS: "iam.amazonaws.com",
                 SpanAttributes.SERVER_PORT: 443,
-                "aws.region": "aws-global",
+                CLOUD_REGION: "aws-global",
             },
         )