diff --git a/instrumentation/opentelemetry-instrumentation-starlette/src/opentelemetry/instrumentation/starlette/__init__.py b/instrumentation/opentelemetry-instrumentation-starlette/src/opentelemetry/instrumentation/starlette/__init__.py
index e241c936a4..3a88582ecf 100644
--- a/instrumentation/opentelemetry-instrumentation-starlette/src/opentelemetry/instrumentation/starlette/__init__.py
+++ b/instrumentation/opentelemetry-instrumentation-starlette/src/opentelemetry/instrumentation/starlette/__init__.py
@@ -192,7 +192,9 @@ def client_response_hook(span: Span, scope: dict[str, Any], message: dict[str, A
 from opentelemetry.instrumentation.starlette.package import _instruments
 from opentelemetry.instrumentation.starlette.version import __version__
 from opentelemetry.metrics import MeterProvider, get_meter
-from opentelemetry.semconv.trace import SpanAttributes
+from opentelemetry.semconv._incubating.attributes.http_attributes import (
+    HTTP_ROUTE,
+)
 from opentelemetry.trace import TracerProvider, get_tracer
 from opentelemetry.util.http import get_excluded_urls
 
@@ -374,7 +376,7 @@ def _get_default_span_details(
     method: str = scope.get("method", "")
     attributes: dict[str, Any] = {}
     if route:
-        attributes[SpanAttributes.HTTP_ROUTE] = route
+        attributes[HTTP_ROUTE] = route
     if method and route:  # http
         span_name = f"{method} {route}"
     elif route:  # websocket
diff --git a/instrumentation/opentelemetry-instrumentation-starlette/tests/test_starlette_instrumentation.py b/instrumentation/opentelemetry-instrumentation-starlette/tests/test_starlette_instrumentation.py
index 5c0f27c02b..17f79073a6 100644
--- a/instrumentation/opentelemetry-instrumentation-starlette/tests/test_starlette_instrumentation.py
+++ b/instrumentation/opentelemetry-instrumentation-starlette/tests/test_starlette_instrumentation.py
@@ -28,7 +28,12 @@
     NumberDataPoint,
 )
 from opentelemetry.sdk.resources import Resource
-from opentelemetry.semconv.trace import SpanAttributes
+from opentelemetry.semconv._incubating.attributes.http_attributes import (
+    HTTP_FLAVOR,
+    HTTP_ROUTE,
+    HTTP_TARGET,
+    HTTP_URL,
+)
 from opentelemetry.test.globals_test import reset_trace_globals
 from opentelemetry.test.test_base import TestBase
 from opentelemetry.trace import (
@@ -122,22 +127,17 @@ def test_sub_app_starlette_call(self):
         spans_with_http_attributes = [
             span
             for span in spans
-            if (
-                SpanAttributes.HTTP_URL in span.attributes
-                or SpanAttributes.HTTP_TARGET in span.attributes
-            )
+            if (HTTP_URL in span.attributes or HTTP_TARGET in span.attributes)
         ]
 
         # expect only one span to have the attributes
         self.assertEqual(1, len(spans_with_http_attributes))
 
         for span in spans_with_http_attributes:
-            self.assertEqual(
-                "/sub/home", span.attributes[SpanAttributes.HTTP_TARGET]
-            )
+            self.assertEqual("/sub/home", span.attributes[HTTP_TARGET])
             self.assertEqual(
                 "http://testserver/sub/home",
-                span.attributes[SpanAttributes.HTTP_URL],
+                span.attributes[HTTP_URL],
             )
 
     def test_starlette_route_attribute_added(self):
@@ -147,14 +147,10 @@ def test_starlette_route_attribute_added(self):
         self.assertEqual(len(spans), 3)
         for span in spans:
             self.assertIn("GET /user/{username}", span.name)
-        self.assertEqual(
-            spans[-1].attributes[SpanAttributes.HTTP_ROUTE], "/user/{username}"
-        )
+        self.assertEqual(spans[-1].attributes[HTTP_ROUTE], "/user/{username}")
         # ensure that at least one attribute that is populated by
         # the asgi instrumentation is successfully feeding though.
-        self.assertEqual(
-            spans[-1].attributes[SpanAttributes.HTTP_FLAVOR], "1.1"
-        )
+        self.assertEqual(spans[-1].attributes[HTTP_FLAVOR], "1.1")
 
     def test_starlette_excluded_urls(self):
         """Ensure that given starlette routes are excluded."""
@@ -456,10 +452,7 @@ def test_sub_app_starlette_call(self):
         spans_with_http_attributes = [
             span
             for span in spans
-            if (
-                SpanAttributes.HTTP_URL in span.attributes
-                or SpanAttributes.HTTP_TARGET in span.attributes
-            )
+            if (HTTP_URL in span.attributes or HTTP_TARGET in span.attributes)
         ]
 
         # We now expect spans with attributes from both the app and its sub app
@@ -480,12 +473,10 @@ def test_sub_app_starlette_call(self):
         self.assertIsNotNone(server_span)
         # As soon as the bug is fixed for starlette, we can iterate over spans_with_http_attributes here
         # to verify the correctness of the attributes for the internal span as well
-        self.assertEqual(
-            "/sub/home", server_span.attributes[SpanAttributes.HTTP_TARGET]
-        )
+        self.assertEqual("/sub/home", server_span.attributes[HTTP_TARGET])
         self.assertEqual(
             "http://testserver/sub/home",
-            server_span.attributes[SpanAttributes.HTTP_URL],
+            server_span.attributes[HTTP_URL],
         )
 
 
@@ -542,10 +533,7 @@ def test_sub_app_starlette_call(self):
         spans_with_http_attributes = [
             span
             for span in spans
-            if (
-                SpanAttributes.HTTP_URL in span.attributes
-                or SpanAttributes.HTTP_TARGET in span.attributes
-            )
+            if (HTTP_URL in span.attributes or HTTP_TARGET in span.attributes)
         ]
 
         # We now expect spans with attributes from both the app and its sub app
@@ -566,12 +554,10 @@ def test_sub_app_starlette_call(self):
         self.assertIsNotNone(server_span)
         # As soon as the bug is fixed for starlette, we can iterate over spans_with_http_attributes here
         # to verify the correctness of the attributes for the internal span as well
-        self.assertEqual(
-            "/sub/home", server_span.attributes[SpanAttributes.HTTP_TARGET]
-        )
+        self.assertEqual("/sub/home", server_span.attributes[HTTP_TARGET])
         self.assertEqual(
             "http://testserver/sub/home",
-            server_span.attributes[SpanAttributes.HTTP_URL],
+            server_span.attributes[HTTP_URL],
         )