diff --git a/CHANGELOG.md b/CHANGELOG.md
index 95d22a4600..8b11e4f92b 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -44,6 +44,8 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
   ([#3673](https://github.com/open-telemetry/opentelemetry-python-contrib/pull/3673))
 - `opentelemetry-instrumentation-starlette`/`opentelemetry-instrumentation-fastapi`: Fixes a crash when host-based routing is used
   ([#3507](https://github.com/open-telemetry/opentelemetry-python-contrib/pull/3507))
+- `opentelemetry-instrumentation-django`: Fixes invalid type at WSGI request headers and attributes collection.
+  ([#3731](https://github.com/open-telemetry/opentelemetry-python-contrib/pull/3731))
 - Fix documentation order of sections and headers for Django, Flask, MySQL, mysqlclient, psycopg, psycopg2, pymysql, sqlalchemy instrumentations.
   ([#3719](https://github.com/open-telemetry/opentelemetry-python-contrib/pull/3719))
 - `opentelemetry-instrumentation-asgi` Fixed an issue where FastAPI reports IP instead of URL.
diff --git a/instrumentation/opentelemetry-instrumentation-django/src/opentelemetry/instrumentation/django/middleware/otel_middleware.py b/instrumentation/opentelemetry-instrumentation-django/src/opentelemetry/instrumentation/django/middleware/otel_middleware.py
index f607046959..1b7d09ee45 100644
--- a/instrumentation/opentelemetry-instrumentation-django/src/opentelemetry/instrumentation/django/middleware/otel_middleware.py
+++ b/instrumentation/opentelemetry-instrumentation-django/src/opentelemetry/instrumentation/django/middleware/otel_middleware.py
@@ -165,6 +165,21 @@ class _DjangoMiddleware(MiddlewareMixin):
         None
     )
 
+    @staticmethod
+    def format_request_objects_in_headers(attributes):
+        for _, value_list in attributes.items():
+            for index, value in enumerate(value_list):
+                if isinstance(value, HttpRequest):
+                    try:
+                        method = getattr(value, "method", "UNKNOWN")
+                        request_path = getattr(value, "path", "UNKNOWN")
+                        value_list[index] = (
+                            f"HttpRequest({method} {request_path})"
+                        )
+                    except Exception:  # pylint: disable=broad-exception-caught
+                        value_list[index] = "HttpRequest(...)"
+        return attributes
+
     @staticmethod
     def _get_span_name(request):
         method = sanitize_method(request.method.strip())
@@ -276,6 +291,11 @@ def process_request(self, request):
                     custom_attributes = (
                         wsgi_collect_custom_request_headers_attributes(carrier)
                     )
+                    # Process custom attributes to handle WSGIRequest objects
+                    custom_attributes = self.format_request_objects_in_headers(
+                        custom_attributes
+                    )
+
                     if len(custom_attributes) > 0:
                         span.set_attributes(custom_attributes)
 
diff --git a/instrumentation/opentelemetry-instrumentation-django/tests/test_middleware.py b/instrumentation/opentelemetry-instrumentation-django/tests/test_middleware.py
index 960bf97bc4..a5c9dcea1d 100644
--- a/instrumentation/opentelemetry-instrumentation-django/tests/test_middleware.py
+++ b/instrumentation/opentelemetry-instrumentation-django/tests/test_middleware.py
@@ -20,6 +20,7 @@
 from unittest.mock import Mock, patch
 
 from django import VERSION, conf
+from django.core.handlers.wsgi import WSGIRequest
 from django.http import HttpRequest, HttpResponse
 from django.test.client import Client
 from django.test.utils import setup_test_environment, teardown_test_environment
@@ -1019,6 +1020,100 @@ def tearDownClass(cls):
         super().tearDownClass()
         conf.settings = conf.LazySettings()
 
+    def test_wsgi_request_in_header_is_properly_formatted(self):
+        mock_wsgi_request = Mock(spec=WSGIRequest)
+        mock_wsgi_request.method = "GET"
+        mock_wsgi_request.path = "/test/path"
+        mock_wsgi_request.__class__.__name__ = "WSGIRequest"
+
+        input_attributes = {
+            "http.request.header.test_wsgirequest_header": [mock_wsgi_request]
+        }
+        expected_attributes = {
+            "http.request.header.test_wsgirequest_header": [
+                "HttpRequest(GET /test/path)"
+            ]
+        }
+
+        formatted_attributes = (
+            _DjangoMiddleware.format_request_objects_in_headers(
+                input_attributes
+            )
+        )
+
+        self.assertEqual(formatted_attributes, expected_attributes)
+
+    def test_wsgi_request_handles_extraction_error(self):
+        mock_wsgi_request = Mock(spec=WSGIRequest)
+        mock_wsgi_request.__class__.__name__ = "WSGIRequest"
+
+        type(mock_wsgi_request).method = property(
+            lambda self: (_ for _ in ()).throw(ValueError("Test error"))
+        )
+
+        input_attributes = {
+            "http.request.header.test_wsgirequest_header": [mock_wsgi_request]
+        }
+        expected_attributes = {
+            "http.request.header.test_wsgirequest_header": ["HttpRequest(...)"]
+        }
+
+        formatted_attributes = (
+            _DjangoMiddleware.format_request_objects_in_headers(
+                input_attributes
+            )
+        )
+
+        self.assertEqual(formatted_attributes, expected_attributes)
+
+    def test_handles_http_request_as_well(self):
+        mock_http_request = Mock(spec=HttpRequest)
+        mock_http_request.method = "POST"
+        mock_http_request.path = "/api/data"
+        mock_http_request.__class__.__name__ = "HttpRequest"
+
+        input_attributes = {
+            "http.request.header.test_httprequest_header": [mock_http_request]
+        }
+        expected_attributes = {
+            "http.request.header.test_httprequest_header": [
+                "HttpRequest(POST /api/data)"
+            ]
+        }
+
+        formatted_attributes = (
+            _DjangoMiddleware.format_request_objects_in_headers(
+                input_attributes
+            )
+        )
+
+        self.assertEqual(formatted_attributes, expected_attributes)
+
+    def test_regular_header_values_are_preserved(self):
+        mock_wsgi_request = Mock(spec=WSGIRequest)
+        mock_wsgi_request.method = "GET"
+        mock_wsgi_request.path = "/test/path"
+        mock_wsgi_request.__class__.__name__ = "WSGIRequest"
+
+        input_attributes = {
+            "http.request.header.test_wsgirequest_header": [mock_wsgi_request],
+            "http.request.header.test_regular_header": ["regular-value"],
+        }
+        expected_attributes = {
+            "http.request.header.test_wsgirequest_header": [
+                "HttpRequest(GET /test/path)"
+            ],
+            "http.request.header.test_regular_header": ["regular-value"],
+        }
+
+        formatted_attributes = (
+            _DjangoMiddleware.format_request_objects_in_headers(
+                input_attributes
+            )
+        )
+
+        self.assertEqual(formatted_attributes, expected_attributes)
+
     def test_http_custom_request_headers_in_span_attributes(self):
         expected = {
             "http.request.header.custom_test_header_1": (