diff --git a/CHANGELOG.md b/CHANGELOG.md
index 024990c91d..8692ebb707 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -11,6 +11,10 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 
 ## Unreleased
 
+### Fixed
+
+- Replace Python 3.14-deprecated `asyncio.iscoroutinefunction` with `inspect.iscoroutinefunction`. ([#3880](https://github.com/open-telemetry/opentelemetry-python-contrib/pull/3880))
+
 ## Version 1.38.0/0.59b0 (2025-10-16)
 
 ### Fixed
diff --git a/instrumentation/opentelemetry-instrumentation-aiokafka/src/opentelemetry/instrumentation/aiokafka/__init__.py b/instrumentation/opentelemetry-instrumentation-aiokafka/src/opentelemetry/instrumentation/aiokafka/__init__.py
index 32837b23c5..d4662c9329 100644
--- a/instrumentation/opentelemetry-instrumentation-aiokafka/src/opentelemetry/instrumentation/aiokafka/__init__.py
+++ b/instrumentation/opentelemetry-instrumentation-aiokafka/src/opentelemetry/instrumentation/aiokafka/__init__.py
@@ -95,7 +95,7 @@ async def produce():
 
 from __future__ import annotations
 
-from asyncio import iscoroutinefunction
+from inspect import iscoroutinefunction
 from typing import TYPE_CHECKING, Collection
 
 import aiokafka
diff --git a/instrumentation/opentelemetry-instrumentation-aiopg/src/opentelemetry/instrumentation/aiopg/aiopg_integration.py b/instrumentation/opentelemetry-instrumentation-aiopg/src/opentelemetry/instrumentation/aiopg/aiopg_integration.py
index 4e6257fbb1..948050818c 100644
--- a/instrumentation/opentelemetry-instrumentation-aiopg/src/opentelemetry/instrumentation/aiopg/aiopg_integration.py
+++ b/instrumentation/opentelemetry-instrumentation-aiopg/src/opentelemetry/instrumentation/aiopg/aiopg_integration.py
@@ -1,4 +1,4 @@
-import asyncio
+import inspect
 import typing
 from collections.abc import Coroutine
 
@@ -197,7 +197,7 @@ async def __aenter__(self):
 
     async def __aexit__(self, exc_type, exc, t_b):
         try:
-            if asyncio.iscoroutinefunction(self._obj.close):
+            if inspect.iscoroutinefunction(self._obj.close):
                 await self._obj.close()
             else:
                 self._obj.close()
diff --git a/instrumentation/opentelemetry-instrumentation-httpx/src/opentelemetry/instrumentation/httpx/__init__.py b/instrumentation/opentelemetry-instrumentation-httpx/src/opentelemetry/instrumentation/httpx/__init__.py
index c93f9b71c5..2a27c98f77 100644
--- a/instrumentation/opentelemetry-instrumentation-httpx/src/opentelemetry/instrumentation/httpx/__init__.py
+++ b/instrumentation/opentelemetry-instrumentation-httpx/src/opentelemetry/instrumentation/httpx/__init__.py
@@ -207,7 +207,7 @@ async def async_response_hook(span, request, response):
 
 import logging
 import typing
-from asyncio import iscoroutinefunction
+from inspect import iscoroutinefunction
 from functools import partial
 from timeit import default_timer
 from types import TracebackType
diff --git a/instrumentation/opentelemetry-instrumentation-httpx/tests/test_httpx_integration.py b/instrumentation/opentelemetry-instrumentation-httpx/tests/test_httpx_integration.py
index 2812aa7a2f..9f40f26dcf 100644
--- a/instrumentation/opentelemetry-instrumentation-httpx/tests/test_httpx_integration.py
+++ b/instrumentation/opentelemetry-instrumentation-httpx/tests/test_httpx_integration.py
@@ -16,6 +16,7 @@
 
 import abc
 import asyncio
+import inspect
 import typing
 from unittest import mock
 
@@ -1046,7 +1047,7 @@ def test_custom_tracer_provider(self):
         def test_response_hook(self):
             response_hook_key = (
                 "async_response_hook"
-                if asyncio.iscoroutinefunction(self.response_hook)
+                if inspect.iscoroutinefunction(self.response_hook)
                 else "response_hook"
             )
             response_hook_kwargs = {response_hook_key: self.response_hook}
@@ -1093,7 +1094,7 @@ def test_response_hook_sync_async_kwargs(self):
         def test_request_hook(self):
             request_hook_key = (
                 "async_request_hook"
-                if asyncio.iscoroutinefunction(self.request_hook)
+                if inspect.iscoroutinefunction(self.request_hook)
                 else "request_hook"
             )
             request_hook_kwargs = {request_hook_key: self.request_hook}