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Add clustermap to sys path to fix import error
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+13
-8
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  • src/methods_transcript_assignment/clustermap

1 file changed

+13
-8
lines changed

src/methods_transcript_assignment/clustermap/script.py

Lines changed: 13 additions & 8 deletions
Original file line numberDiff line numberDiff line change
@@ -4,9 +4,12 @@
44
import spatialdata as sd
55
import anndata as ad
66
import pandas as pd
7+
import sys
78
import os
89
import shutil
910

11+
sys.path.insert(0, os.path.abspath("/ClusterMap"))
12+
1013
import time
1114
from datetime import timedelta
1215
import math
@@ -76,7 +79,7 @@ def run_clustermap_over_chunks(
7679
):
7780
"""
7881
"""
79-
82+
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# Infer parameters
8184
num_gene=np.max(spots['gene'])
8285
num_dims = len(dapi.shape)
@@ -102,7 +105,7 @@ def run_clustermap_over_chunks(
102105

103106
if spots_tile.shape[0] < 20:
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continue
105-
108+
106109
# Instantiate model for tile
107110
t0_model = time.time()
108111
model_tile = ClusterMap(
@@ -111,14 +114,14 @@ def run_clustermap_over_chunks(
111114
)
112115
time_model = timedelta(seconds=(time.time() - t0_model))
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114-
117+
115118
# Preprocessing
116119
t0_prepro = time.time()
117120
model_tile.preprocess(
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dapi_grid_interval=dapi_grid_interval, pct_filter=pct_filter, LOF=LOF, contamination=contamination
119122
)
120123
time_prepro = timedelta(seconds=(time.time() - t0_prepro))
121-
124+
122125
# Segmentation
123126
model_tile.min_spot_per_cell=min_spot_per_cell
124127
t0_segment = time.time()
@@ -143,7 +146,7 @@ def run_clustermap_over_chunks(
143146
flush=True
144147
)
145148
#TODO: include an assertion for an expected_time < 24 h? And a recommendation to decrease dapi_grid_interval?/data_size
146-
149+
147150

148151
return model.spots
149152

@@ -164,7 +167,7 @@ def run_clustermap(
164167
spots=spots, dapi=dapi, gene_list=gene_list, num_dims=num_dims, xy_radius=xy_radius, z_radius=z_radius,
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fast_preprocess=fast_preprocess, gauss_blur=gauss_blur, sigma=sigma
166169
)
167-
170+
168171
# Preprocessing
169172
model.preprocess(dapi_grid_interval=dapi_grid_interval, pct_filter=pct_filter, LOF=LOF, contamination=contamination)
170173

@@ -174,7 +177,7 @@ def run_clustermap(
174177
cell_num_threshold=cell_num_threshold, dapi_grid_interval=dapi_grid_interval, add_dapi=add_dapi,
175178
use_genedis=use_genedis
176179
)
177-
180+
178181
return model.spots
179182

180183

@@ -208,7 +211,8 @@ def run_clustermap(
208211
if isinstance(sdata_segm["segmentation"], xr.DataTree):
209212
label_image = sdata_segm["segmentation"]["scale0"].image.to_numpy()
210213
else:
211-
label_image = sdata_segm["segmentation"].to_numpy()
214+
label_image = sdata_segm["segmentation"].to_numpy()
215+
212216
dapi_image = np.squeeze(sdata['morphology_mip']['scale0']['image'].compute())
213217

214218
# Extract coordinates and feature names (= gene names) from SpatialData
@@ -297,4 +301,5 @@ def run_clustermap(
297301
print('Write transcripts with cell ids', flush=True)
298302
if os.path.exists(par["output"]):
299303
shutil.rmtree(par["output"])
304+
300305
sdata_transcripts_only.write(par['output'])

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