How can I calculate the average intensity within a specific ROI?

[yongtaektab-cmyk]

Hello everyone,

I am new to Bonsai and I'm trying to create a simple workflow. My goal is to:

1. Capture an image.

2. Convert it to grayscale and crop it.

3. Define an ROI.

4. Calculate the average pixel intensity within that ROI.

I’ve set up the nodes for capturing and cropping, but whenever I add an 'Average' node to get the intensity, the workflow fails or throws an error.

Could anyone please guide me on how to correctly configure the nodes for this? I’m not sure if I’m missing a step or using the wrong node type. Any advice would be greatly appreciated!

[banchan86]

Hi @yongtaektab-cmyk! You are nearly there. So RoiActivity actually already does the calculation, you can configure the Operation property to change the calculation method (e.g. Sum or Average). You also have to first define the ROIs, check out the documentation for RoiActivity in the link above. If you did it right, double clicking on RoiActivity should show the visualizer with the intensity readout for each ROI, and it will also be saved in the .csv.

[yongtaektab-cmyk]

Thank you so much!