diff --git a/pandas-stubs/core/frame.pyi b/pandas-stubs/core/frame.pyi
index 63f5acab..784f6f09 100644
--- a/pandas-stubs/core/frame.pyi
+++ b/pandas-stubs/core/frame.pyi
@@ -1148,7 +1148,6 @@ class DataFrame(NDFrame, OpsMixin, _GetItemHack):
     def groupby(  # pyright: ignore reportOverlappingOverload
         self,
         by: Scalar,
-        axis: AxisIndex | _NoDefaultDoNotUse = ...,
         level: IndexLabel | None = ...,
         as_index: Literal[True] = True,
         sort: _bool = ...,
@@ -1160,7 +1159,6 @@ class DataFrame(NDFrame, OpsMixin, _GetItemHack):
     def groupby(
         self,
         by: Scalar,
-        axis: AxisIndex | _NoDefaultDoNotUse = ...,
         level: IndexLabel | None = ...,
         as_index: Literal[False] = ...,
         sort: _bool = ...,
@@ -1172,7 +1170,6 @@ class DataFrame(NDFrame, OpsMixin, _GetItemHack):
     def groupby(  # pyright: ignore reportOverlappingOverload
         self,
         by: DatetimeIndex,
-        axis: AxisIndex | _NoDefaultDoNotUse = ...,
         level: IndexLabel | None = ...,
         as_index: Literal[True] = True,
         sort: _bool = ...,
@@ -1184,7 +1181,6 @@ class DataFrame(NDFrame, OpsMixin, _GetItemHack):
     def groupby(  # pyright: ignore reportOverlappingOverload
         self,
         by: DatetimeIndex,
-        axis: AxisIndex | _NoDefaultDoNotUse = ...,
         level: IndexLabel | None = ...,
         as_index: Literal[False] = ...,
         sort: _bool = ...,
@@ -1196,7 +1192,6 @@ class DataFrame(NDFrame, OpsMixin, _GetItemHack):
     def groupby(  # pyright: ignore reportOverlappingOverload
         self,
         by: TimedeltaIndex,
-        axis: AxisIndex | _NoDefaultDoNotUse = ...,
         level: IndexLabel | None = ...,
         as_index: Literal[True] = True,
         sort: _bool = ...,
@@ -1208,7 +1203,6 @@ class DataFrame(NDFrame, OpsMixin, _GetItemHack):
     def groupby(
         self,
         by: TimedeltaIndex,
-        axis: AxisIndex | _NoDefaultDoNotUse = ...,
         level: IndexLabel | None = ...,
         as_index: Literal[False] = ...,
         sort: _bool = ...,
@@ -1220,7 +1214,6 @@ class DataFrame(NDFrame, OpsMixin, _GetItemHack):
     def groupby(  # pyright: ignore reportOverlappingOverload
         self,
         by: PeriodIndex,
-        axis: AxisIndex | _NoDefaultDoNotUse = ...,
         level: IndexLabel | None = ...,
         as_index: Literal[True] = True,
         sort: _bool = ...,
@@ -1232,7 +1225,6 @@ class DataFrame(NDFrame, OpsMixin, _GetItemHack):
     def groupby(
         self,
         by: PeriodIndex,
-        axis: AxisIndex | _NoDefaultDoNotUse = ...,
         level: IndexLabel | None = ...,
         as_index: Literal[False] = ...,
         sort: _bool = ...,
@@ -1244,7 +1236,6 @@ class DataFrame(NDFrame, OpsMixin, _GetItemHack):
     def groupby(  # pyright: ignore reportOverlappingOverload
         self,
         by: IntervalIndex[IntervalT],
-        axis: AxisIndex | _NoDefaultDoNotUse = ...,
         level: IndexLabel | None = ...,
         as_index: Literal[True] = True,
         sort: _bool = ...,
@@ -1256,7 +1247,6 @@ class DataFrame(NDFrame, OpsMixin, _GetItemHack):
     def groupby(
         self,
         by: IntervalIndex[IntervalT],
-        axis: AxisIndex | _NoDefaultDoNotUse = ...,
         level: IndexLabel | None = ...,
         as_index: Literal[False] = ...,
         sort: _bool = ...,
@@ -1268,7 +1258,6 @@ class DataFrame(NDFrame, OpsMixin, _GetItemHack):
     def groupby(  # type: ignore[overload-overlap] # pyright: ignore reportOverlappingOverload
         self,
         by: MultiIndex | GroupByObjectNonScalar | None = ...,
-        axis: AxisIndex | _NoDefaultDoNotUse = ...,
         level: IndexLabel | None = ...,
         as_index: Literal[True] = True,
         sort: _bool = ...,
@@ -1280,7 +1269,6 @@ class DataFrame(NDFrame, OpsMixin, _GetItemHack):
     def groupby(  # type: ignore[overload-overlap]
         self,
         by: MultiIndex | GroupByObjectNonScalar | None = ...,
-        axis: AxisIndex | _NoDefaultDoNotUse = ...,
         level: IndexLabel | None = ...,
         as_index: Literal[False] = ...,
         sort: _bool = ...,
@@ -1292,7 +1280,6 @@ class DataFrame(NDFrame, OpsMixin, _GetItemHack):
     def groupby(  # pyright: ignore reportOverlappingOverload
         self,
         by: Series[SeriesByT],
-        axis: AxisIndex | _NoDefaultDoNotUse = ...,
         level: IndexLabel | None = ...,
         as_index: Literal[True] = True,
         sort: _bool = ...,
@@ -1304,7 +1291,6 @@ class DataFrame(NDFrame, OpsMixin, _GetItemHack):
     def groupby(
         self,
         by: Series[SeriesByT],
-        axis: AxisIndex | _NoDefaultDoNotUse = ...,
         level: IndexLabel | None = ...,
         as_index: Literal[False] = ...,
         sort: _bool = ...,
@@ -1316,7 +1302,6 @@ class DataFrame(NDFrame, OpsMixin, _GetItemHack):
     def groupby(
         self,
         by: CategoricalIndex | Index | Series,
-        axis: AxisIndex | _NoDefaultDoNotUse = ...,
         level: IndexLabel | None = ...,
         as_index: Literal[True] = True,
         sort: _bool = ...,
@@ -1328,7 +1313,6 @@ class DataFrame(NDFrame, OpsMixin, _GetItemHack):
     def groupby(
         self,
         by: CategoricalIndex | Index | Series,
-        axis: AxisIndex | _NoDefaultDoNotUse = ...,
         level: IndexLabel | None = ...,
         as_index: Literal[False] = ...,
         sort: _bool = ...,
diff --git a/pandas-stubs/core/series.pyi b/pandas-stubs/core/series.pyi
index d206adcb..bd6b2dd5 100644
--- a/pandas-stubs/core/series.pyi
+++ b/pandas-stubs/core/series.pyi
@@ -626,7 +626,6 @@ class Series(IndexOpsMixin[S1], NDFrame):
     def groupby(
         self,
         by: Scalar,
-        axis: AxisIndex = ...,
         level: IndexLabel | None = ...,
         as_index: _bool = ...,
         sort: _bool = ...,
@@ -638,7 +637,6 @@ class Series(IndexOpsMixin[S1], NDFrame):
     def groupby(
         self,
         by: DatetimeIndex,
-        axis: AxisIndex = ...,
         level: IndexLabel | None = ...,
         as_index: _bool = ...,
         sort: _bool = ...,
@@ -650,7 +648,6 @@ class Series(IndexOpsMixin[S1], NDFrame):
     def groupby(
         self,
         by: TimedeltaIndex,
-        axis: AxisIndex = ...,
         level: IndexLabel | None = ...,
         as_index: _bool = ...,
         sort: _bool = ...,
@@ -662,7 +659,6 @@ class Series(IndexOpsMixin[S1], NDFrame):
     def groupby(
         self,
         by: PeriodIndex,
-        axis: AxisIndex = ...,
         level: IndexLabel | None = ...,
         as_index: _bool = ...,
         sort: _bool = ...,
@@ -674,7 +670,6 @@ class Series(IndexOpsMixin[S1], NDFrame):
     def groupby(
         self,
         by: IntervalIndex[IntervalT],
-        axis: AxisIndex = ...,
         level: IndexLabel | None = ...,
         as_index: _bool = ...,
         sort: _bool = ...,
@@ -686,7 +681,6 @@ class Series(IndexOpsMixin[S1], NDFrame):
     def groupby(
         self,
         by: MultiIndex | GroupByObjectNonScalar,
-        axis: AxisIndex = ...,
         level: IndexLabel | None = ...,
         as_index: _bool = ...,
         sort: _bool = ...,
@@ -698,7 +692,6 @@ class Series(IndexOpsMixin[S1], NDFrame):
     def groupby(
         self,
         by: None,
-        axis: AxisIndex,
         level: IndexLabel,  # level is required when by=None (passed as positional)
         as_index: _bool = ...,
         sort: _bool = ...,
@@ -710,7 +703,6 @@ class Series(IndexOpsMixin[S1], NDFrame):
     def groupby(
         self,
         by: None = ...,
-        axis: AxisIndex = ...,
         *,
         level: IndexLabel,  # level is required when by=None (passed as keyword)
         as_index: _bool = ...,
@@ -723,7 +715,6 @@ class Series(IndexOpsMixin[S1], NDFrame):
     def groupby(
         self,
         by: Series[SeriesByT],
-        axis: AxisIndex = ...,
         level: IndexLabel | None = ...,
         as_index: _bool = ...,
         sort: _bool = ...,
@@ -735,7 +726,6 @@ class Series(IndexOpsMixin[S1], NDFrame):
     def groupby(
         self,
         by: CategoricalIndex | Index | Series,
-        axis: AxisIndex = ...,
         level: IndexLabel | None = ...,
         as_index: _bool = ...,
         sort: _bool = ...,
diff --git a/tests/test_frame.py b/tests/test_frame.py
index 7d67cfc9..4dc9b82c 100644
--- a/tests/test_frame.py
+++ b/tests/test_frame.py
@@ -688,9 +688,6 @@ def test_types_mean() -> None:
         assert_type(df.mean(axis=1, skipna=True, numeric_only=False), pd.Series),
         pd.Series,
     )
-    if TYPE_CHECKING_INVALID_USAGE:
-        df3: pd.DataFrame = df.groupby(axis=1, level=0).mean()  # type: ignore[call-overload] # pyright: ignore[reportArgumentType, reportCallIssue]
-        df4: pd.DataFrame = df.groupby(axis=1, level=0, dropna=True).mean()  # type: ignore[call-overload] # pyright: ignore[reportArgumentType, reportCallIssue]
 
 
 def test_types_median() -> None:
@@ -702,9 +699,6 @@ def test_types_median() -> None:
         assert_type(df.median(axis=1, skipna=True, numeric_only=False), pd.Series),
         pd.Series,
     )
-    if TYPE_CHECKING_INVALID_USAGE:
-        df3: pd.DataFrame = df.groupby(axis=1, level=0).median()  # type: ignore[call-overload] # pyright: ignore[reportArgumentType, reportCallIssue]
-        df4: pd.DataFrame = df.groupby(axis=1, level=0, dropna=True).median()  # type: ignore[call-overload] # pyright: ignore[reportArgumentType, reportCallIssue]
 
 
 def test_types_iterrows() -> None: