diff --git a/pytest_asyncio/plugin.py b/pytest_asyncio/plugin.py
index 38b3e28a..4f4dbcee 100644
--- a/pytest_asyncio/plugin.py
+++ b/pytest_asyncio/plugin.py
@@ -442,8 +442,25 @@ def _can_substitute(item: Function) -> bool:
         """Returns whether the specified function can be replaced by this class"""
         raise NotImplementedError()
 
+    def setup(self) -> None:
+        runner_fixture_id = f"_{self._loop_scope}_scoped_runner"
+        if runner_fixture_id not in self.fixturenames:
+            self.fixturenames.append(runner_fixture_id)
+        return super().setup()
+
+    def runtest(self) -> None:
+        runner_fixture_id = f"_{self._loop_scope}_scoped_runner"
+        runner = self._request.getfixturevalue(runner_fixture_id)
+        context = contextvars.copy_context()
+        synchronized_obj = _synchronize_coroutine(
+            getattr(*self._synchronization_target_attr), runner, context
+        )
+        with MonkeyPatch.context() as c:
+            c.setattr(*self._synchronization_target_attr, synchronized_obj)
+            super().runtest()
+
     @functools.cached_property
-    def loop_scope(self) -> _ScopeName:
+    def _loop_scope(self) -> _ScopeName:
         """
         Return the scope of the asyncio event loop this item is run in.
 
@@ -457,17 +474,6 @@ def loop_scope(self) -> _ScopeName:
         default_loop_scope = _get_default_test_loop_scope(self.config)
         return _get_marked_loop_scope(marker, default_loop_scope)
 
-    def runtest(self) -> None:
-        runner_fixture_id = f"_{self.loop_scope}_scoped_runner"
-        runner = self._request.getfixturevalue(runner_fixture_id)
-        context = contextvars.copy_context()
-        synchronized_obj = _synchronize_coroutine(
-            getattr(*self._synchronization_target_attr), runner, context
-        )
-        with MonkeyPatch.context() as c:
-            c.setattr(*self._synchronization_target_attr, synchronized_obj)
-            super().runtest()
-
     @property
     def _synchronization_target_attr(self) -> tuple[object, str]:
         """
@@ -531,6 +537,16 @@ class AsyncHypothesisTest(PytestAsyncioFunction):
     @hypothesis.given.
     """
 
+    def setup(self) -> None:
+        if not getattr(self.obj, "hypothesis", False) and getattr(
+            self.obj, "is_hypothesis_test", False
+        ):
+            pytest.fail(
+                f"test function `{self!r}` is using Hypothesis, but pytest-asyncio "
+                "only works with Hypothesis 3.64.0 or later."
+            )
+        return super().setup()
+
     @staticmethod
     def _can_substitute(item: Function) -> bool:
         func = item.obj
@@ -693,24 +709,6 @@ def inner(*args, **kwargs):
     return inner
 
 
-def pytest_runtest_setup(item: pytest.Item) -> None:
-    marker = item.get_closest_marker("asyncio")
-    if marker is None or not is_async_test(item):
-        return
-    runner_fixture_id = f"_{item.loop_scope}_scoped_runner"
-    fixturenames = item.fixturenames  # type: ignore[attr-defined]
-    if runner_fixture_id not in fixturenames:
-        fixturenames.append(runner_fixture_id)
-    obj = getattr(item, "obj", None)
-    if not getattr(obj, "hypothesis", False) and getattr(
-        obj, "is_hypothesis_test", False
-    ):
-        pytest.fail(
-            f"test function `{item!r}` is using Hypothesis, but pytest-asyncio "
-            "only works with Hypothesis 3.64.0 or later."
-        )
-
-
 @pytest.hookimpl(wrapper=True)
 def pytest_fixture_setup(fixturedef: FixtureDef, request) -> object | None:
     asyncio_mode = _get_asyncio_mode(request.config)