diff --git a/Include/internal/pycore_interp.h b/Include/internal/pycore_interp.h
index 5e4bcbf835a4d0..87cdcb5b119d15 100644
--- a/Include/internal/pycore_interp.h
+++ b/Include/internal/pycore_interp.h
@@ -283,6 +283,8 @@ struct _is {
 
     /* the initial PyInterpreterState.threads.head */
     _PyThreadStateImpl _initial_thread;
+    // _initial_thread should be the last field of PyInterpreterState.
+    // See https://github.com/python/cpython/issues/127117.
 };
 
 
diff --git a/Include/internal/pycore_runtime.h b/Include/internal/pycore_runtime.h
index 2f2cec22cf1589..86d024535fdda8 100644
--- a/Include/internal/pycore_runtime.h
+++ b/Include/internal/pycore_runtime.h
@@ -169,6 +169,12 @@ typedef struct pyruntimestate {
     struct _Py_unicode_runtime_state unicode_state;
     struct _types_runtime_state types;
 
+#if defined(__EMSCRIPTEN__) && defined(PY_CALL_TRAMPOLINE)
+    // Used in "Python/emscripten_trampoline.c" to choose between type
+    // reflection trampoline and EM_JS trampoline.
+    bool wasm_type_reflection_available;
+#endif
+
     /* All the objects that are shared by the runtime's interpreters. */
     struct _Py_cached_objects cached_objects;
     struct _Py_static_objects static_objects;
@@ -189,13 +195,8 @@ typedef struct pyruntimestate {
 
     /* _PyRuntimeState.interpreters.main */
     PyInterpreterState _main_interpreter;
-
-#if defined(__EMSCRIPTEN__) && defined(PY_CALL_TRAMPOLINE)
-    // Used in "Python/emscripten_trampoline.c" to choose between type
-    // reflection trampoline and EM_JS trampoline.
-    bool wasm_type_reflection_available;
-#endif
-
+    // _main_interpreter should be the last field of _PyRuntimeState.
+    // See https://github.com/python/cpython/issues/127117.
 } _PyRuntimeState;