diff --git a/Lib/multiprocessing/spawn.py b/Lib/multiprocessing/spawn.py
index daac1ecc34b55e..2ac9e28fa968b3 100644
--- a/Lib/multiprocessing/spawn.py
+++ b/Lib/multiprocessing/spawn.py
@@ -170,7 +170,10 @@ def get_preparation_data(name):
     if util._logger is not None:
         d['log_level'] = util._logger.getEffectiveLevel()
 
-    sys_path=sys.path.copy()
+    sys_path=[]
+    for path in sys.path:
+        if sys.base_prefix not in os.path.abspath(path):
+            sys_path.append(path) # For cross-interpreter support
     try:
         i = sys_path.index('')
     except ValueError:
@@ -226,7 +229,11 @@ def prepare(data):
         util.get_logger().setLevel(data['log_level'])
 
     if 'sys_path' in data:
-        sys.path = data['sys_path']
+        sys_path = data['sys_path'].copy()
+        for path in data['sys_path']:
+            if path in sys.path:
+                sys_path.remove(path)
+        sys.path.extend(sys_path) # For cross-interpreter support
 
     if 'sys_argv' in data:
         sys.argv = data['sys_argv']
diff --git a/Misc/NEWS.d/next/Library/2025-03-24-12-37-35.gh-issue-131656.7PiFWH.rst b/Misc/NEWS.d/next/Library/2025-03-24-12-37-35.gh-issue-131656.7PiFWH.rst
new file mode 100644
index 00000000000000..e7c1021d3f2f44
--- /dev/null
+++ b/Misc/NEWS.d/next/Library/2025-03-24-12-37-35.gh-issue-131656.7PiFWH.rst
@@ -0,0 +1 @@
+Fixed the cross-interpreter issue of subprocess module by modifying :func:`multiprocessing.spawn.prepare` and :func:`multiprocessing.spawn.get_preparation_data`. Contributed by pypy66.