diff --git a/docs/api/command-line-switches.md b/docs/api/command-line-switches.md
index 725144c1a4aad..5695b40d3f84f 100644
--- a/docs/api/command-line-switches.md
+++ b/docs/api/command-line-switches.md
@@ -49,6 +49,10 @@ Disables the disk cache for HTTP requests.
 
 Disable HTTP/2 and SPDY/3.1 protocols.
 
+### --disable-geolocation _macOS_
+
+Disables the Geolocation API. Permission requests for geolocation will be denied internally regardless of the decision made by a handler set via `session.setPermissionRequestHandler`. This functionality is currently implemented only for macOS. Has no effect on other platforms.
+
 ### --disable-renderer-backgrounding
 
 Prevents Chromium from lowering the priority of invisible pages' renderer
diff --git a/shell/browser/api/electron_api_session.cc b/shell/browser/api/electron_api_session.cc
index e96a8962cb0b3..1d08c82680506 100644
--- a/shell/browser/api/electron_api_session.cc
+++ b/shell/browser/api/electron_api_session.cc
@@ -884,6 +884,24 @@ void Session::SetPermissionRequestHandler(v8::Local<v8::Value> val,
          blink::PermissionType permission_type,
          ElectronPermissionManager::StatusCallback callback,
          const base::Value& details) {
+#if (BUILDFLAG(IS_MAC))
+        if (permission_type == blink::PermissionType::GEOLOCATION) {
+          if (ElectronPermissionManager::
+                  IsGeolocationDisabledViaCommandLine()) {
+            auto original_callback = std::move(callback);
+            callback = base::BindOnce(
+                [](ElectronPermissionManager::StatusCallback callback,
+                   content::PermissionResult /*ignored_result*/) {
+                  // Always deny regardless of what
+                  // content::PermissionResult is passed here
+                  std::move(callback).Run(content::PermissionResult(
+                      blink::mojom::PermissionStatus::DENIED,
+                      content::PermissionStatusSource::UNSPECIFIED));
+                },
+                std::move(original_callback));
+          }
+        }
+#endif
         handler->Run(web_contents, permission_type, std::move(callback),
                      details);
       },
diff --git a/shell/browser/electron_browser_main_parts_mac.mm b/shell/browser/electron_browser_main_parts_mac.mm
index c7c093010ef0b..2a4238dec285c 100644
--- a/shell/browser/electron_browser_main_parts_mac.mm
+++ b/shell/browser/electron_browser_main_parts_mac.mm
@@ -11,6 +11,7 @@
 #include "services/device/public/cpp/geolocation/geolocation_system_permission_manager.h"
 #include "services/device/public/cpp/geolocation/system_geolocation_source_apple.h"
 #include "shell/browser/browser_process_impl.h"
+#include "shell/browser/electron_permission_manager.h"
 #include "shell/browser/mac/electron_application.h"
 #include "shell/browser/mac/electron_application_delegate.h"
 #include "ui/base/l10n/l10n_util_mac.h"
@@ -32,7 +33,13 @@
       setObject:@"NO"
          forKey:@"NSTreatUnknownArgumentsAsOpen"];
 
-  if (!device::GeolocationSystemPermissionManager::GetInstance()) {
+  const bool geolocationDisabled =
+      ElectronPermissionManager::IsGeolocationDisabledViaCommandLine();
+
+  // Check if geolocation api is NOT disabled via command line before
+  // CreateGeolocationSystemPermissionManager is called
+  if (!geolocationDisabled &&
+      !device::GeolocationSystemPermissionManager::GetInstance()) {
     device::GeolocationSystemPermissionManager::SetInstance(
         device::SystemGeolocationSourceApple::
             CreateGeolocationSystemPermissionManager());
diff --git a/shell/browser/electron_permission_manager.cc b/shell/browser/electron_permission_manager.cc
index a58b3a696302b..c50c76789bbc9 100644
--- a/shell/browser/electron_permission_manager.cc
+++ b/shell/browser/electron_permission_manager.cc
@@ -8,6 +8,7 @@
 #include <utility>
 #include <vector>
 
+#include "base/command_line.h"
 #include "base/containers/to_vector.h"
 #include "base/values.h"
 #include "content/browser/permissions/permission_util.h"  // nogncheck
@@ -146,6 +147,17 @@ void ElectronPermissionManager::SetBluetoothPairingHandler(
   bluetooth_pairing_handler_ = handler;
 }
 
+// static
+bool ElectronPermissionManager::IsGeolocationDisabledViaCommandLine() {
+// Remove platform check once flag is extended to other platforms
+#if BUILDFLAG(IS_MAC)
+  auto* command_line = base::CommandLine::ForCurrentProcess();
+  return command_line->HasSwitch("disable-geolocation");
+#else
+  return false;
+#endif
+}
+
 bool ElectronPermissionManager::HasPermissionRequestHandler() const {
   return !request_handler_.is_null();
 }
@@ -220,9 +232,16 @@ void ElectronPermissionManager::RequestPermissionsWithDetails(
             ->GrantSendMidiSysExMessage(
                 render_frame_host->GetProcess()->GetDeprecatedID());
       } else if (permission_type == blink::PermissionType::GEOLOCATION) {
-        ElectronBrowserMainParts::Get()
-            ->GetGeolocationControl()
-            ->UserDidOptIntoLocationServices();
+        if (IsGeolocationDisabledViaCommandLine()) {
+          results.push_back(content::PermissionResult(
+              blink::mojom::PermissionStatus::DENIED,
+              content::PermissionStatusSource::UNSPECIFIED));
+          continue;
+        } else {
+          ElectronBrowserMainParts::Get()
+              ->GetGeolocationControl()
+              ->UserDidOptIntoLocationServices();
+        }
       }
       results.push_back(content::PermissionResult(
           blink::mojom::PermissionStatus::GRANTED,
@@ -331,6 +350,10 @@ bool ElectronPermissionManager::CheckPermissionWithDetails(
     content::RenderFrameHost* render_frame_host,
     const GURL& requesting_origin,
     base::Value::Dict details) const {
+  if (permission == blink::PermissionType::GEOLOCATION &&
+      IsGeolocationDisabledViaCommandLine())
+    return false;
+
   if (check_handler_.is_null()) {
     if (permission == blink::PermissionType::DEPRECATED_SYNC_CLIPBOARD_READ) {
       return false;
@@ -368,6 +391,10 @@ bool ElectronPermissionManager::CheckDevicePermission(
     const url::Origin& origin,
     const base::Value& device,
     ElectronBrowserContext* browser_context) const {
+  if (permission == blink::PermissionType::GEOLOCATION &&
+      IsGeolocationDisabledViaCommandLine())
+    return false;
+
   if (device_permission_handler_.is_null())
     return browser_context->CheckDevicePermission(origin, device, permission);
 
diff --git a/shell/browser/electron_permission_manager.h b/shell/browser/electron_permission_manager.h
index 6bdcec371c405..9a0062ef631e5 100644
--- a/shell/browser/electron_permission_manager.h
+++ b/shell/browser/electron_permission_manager.h
@@ -66,6 +66,8 @@ class ElectronPermissionManager : public content::PermissionControllerDelegate {
   using BluetoothPairingHandler =
       base::RepeatingCallback<void(gin_helper::Dictionary, PairCallback)>;
 
+  static bool IsGeolocationDisabledViaCommandLine();
+
   void RequestPermissionWithDetails(
       blink::mojom::PermissionDescriptorPtr permission,
       content::RenderFrameHost* render_frame_host,
diff --git a/spec/chromium-spec.ts b/spec/chromium-spec.ts
index 4457d2a1bfb8b..515e5ef5af129 100644
--- a/spec/chromium-spec.ts
+++ b/spec/chromium-spec.ts
@@ -898,6 +898,72 @@ describe('chromium features', () => {
       expect(position).to.have.property('coords');
       expect(position).to.have.property('timestamp');
     });
+
+    ifdescribe(process.platform === 'darwin')('with --disable-geolocation', () => {
+      const testSwitchBehavior = (handlerAction: 'allow' | 'deny' | 'none') => async () => {
+        const rc = await startRemoteControlApp([
+          '--disable-geolocation',
+          `--boot-eval=fixturesPath=${JSON.stringify(fixturesPath)}`
+        ]);
+
+        const result = await rc.remotely(async (action: typeof handlerAction) => {
+          const { session, BrowserWindow } = require('electron');
+          const path = require('node:path');
+
+          // Isolate each test's permissions to prevent permission state leaks between the test variations
+          const testSession = session.fromPartition(`geolocation-disable-${action}`);
+
+          if (action !== 'none') {
+            // Make the PermissionRequestHandler behave according to action variable passed for this test
+            testSession.setPermissionRequestHandler((_wc, permission, callback) => {
+              if (permission === 'geolocation') {
+                if (action === 'allow') callback(true);
+                else if (action === 'deny') callback(false);
+                else callback(false);
+              }
+            });
+          }
+
+          const w = new BrowserWindow({
+            show: false,
+            webPreferences: {
+              session: testSession,
+              nodeIntegration: true,
+              contextIsolation: false
+            }
+          });
+
+          await w.loadFile(path.join(fixturesPath, 'pages', 'blank.html'));
+
+          const permissionState = await w.webContents.executeJavaScript(`
+            navigator.permissions.query({ name: 'geolocation' })
+              .then(status => status.state)
+              .catch(() => 'error')
+          `);
+
+          const geoResult = await w.webContents.executeJavaScript(`
+            new Promise(resolve => {
+              navigator.geolocation.getCurrentPosition(
+                () => resolve('allowed'),
+                err => resolve(err.code)
+              );
+            })
+          `);
+
+          return { permissionState, geoResult };
+        }, handlerAction);
+
+        // Always expect status to be denied regardless of the decision made by a handler set via `session.setPermissionRequestHandler`
+        expect(result.permissionState).to.equal('denied', `Unexpected permission state for ${handlerAction} handler`);
+
+        // 1 = PERMISSION_DENIED
+        expect(result.geoResult).to.equal(1, `Unexpected API result for ${handlerAction} handler`);
+      };
+
+      it('denies geolocation when permission request handler would allow', testSwitchBehavior('allow'));
+      it('denies geolocation when permission request handler would deny', testSwitchBehavior('deny'));
+      it('denies geolocation with no permission request handler', testSwitchBehavior('none'));
+    });
   });
 
   describe('File System API,', () => {