You signed in with another tab or window. Reload to refresh your session.You signed out in another tab or window. Reload to refresh your session.You switched accounts on another tab or window. Reload to refresh your session.Dismiss alert
I have recenlty started to try OPEN-ST. For this we have used a flocell S1 and we have loaded the oligo to a concentration of 200pM as indicated in your materials and methods. Today I got my fastq files and I was surprised to see that we got only 4 million reads per lane...quite far to what we get in a classical sequencing assay...could you please let me know how many reads do you get (I know you did it in a S4 but still in percentage relative to a classical run in S4)?
I guess the main issue is that I may get quite sparse reads over the flowcell , thus the initial theoretical resolution of 0.6 microns might not be reached or...
thanks for your feedback.
reacted with thumbs up emoji reacted with thumbs down emoji reacted with laugh emoji reacted with hooray emoji reacted with confused emoji reacted with heart emoji reacted with rocket emoji reacted with eyes emoji
Uh oh!
There was an error while loading. Please reload this page.
-
Dear OPEN-ST members;
I have recenlty started to try OPEN-ST. For this we have used a flocell S1 and we have loaded the oligo to a concentration of 200pM as indicated in your materials and methods. Today I got my fastq files and I was surprised to see that we got only 4 million reads per lane...quite far to what we get in a classical sequencing assay...could you please let me know how many reads do you get (I know you did it in a S4 but still in percentage relative to a classical run in S4)?
I guess the main issue is that I may get quite sparse reads over the flowcell , thus the initial theoretical resolution of 0.6 microns might not be reached or...
thanks for your feedback.
regards
Marco Mendoza
Beta Was this translation helpful? Give feedback.
All reactions