README.md

Orange workflows for Agilent µ-FTIR spectroscopy data processing

In this repository you find Orange workflows for processing Agilent µ-FTIR data. An example dataset including hyperspectral example data.

Guidelines for downloading and oppening workflows:

1. Go to IMBUIA - IR_microspectroscopy_workflows
2. Click on Code and then Download ZIP. This can take some time...
3. You should get the following files structure:

├───README.md
│
├───dataset
│   ├── hyper_sample_data.bsp
│   └── hyper_sample_data.dat
│
├───img
├───workflows
│   └── agilent_hyperspectral_workflow.ows

5. Open the Orange app (for Orange installation, access the links Quasar or Orange. It is mandatory to have the Spectroscopy Add-On installed for this kind of data processing.);
6. Load the workflow](workflows/).

---

Workflows for processing FPA Agilent data

1. Processing data (*.dat)

To process Agilent image spectrum, it is mandatory that the following files should be in the same folder:

└── data_folder
│   ├── hyper_sample_data.bsp
│   └── hyper_sample_data.dat

Files content and designation:

• .dat contains the experimental data of the measurement;
• .bsp file contains the experimental parameters of the measurement (metadata).

A typical workflow for processing *.dat files is presented:

Loading procedure:

1. Double click on the Multifile widget, click on Clear and then on the folder to select the data from the dataset;
2. Define the specific reader Spectra ASCII or Agilent Single Tile Image (*.dat) and then select the *.dat file in the folder;
3. Files are loaded and the workflow is ready. To double check the loading procedure, click on the visualization widgets (Hyperspectra). The spectral data should be displayed.