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docs: document WarpDemuX adapter sets, naming convention, and upstream repo
Add WarpDemuX repo link, model naming convention breakdown, expanded kit
table, and info admonition about standard vs tRNA-specific models.
Co-Authored-By: Claude Opus 4.6 <noreply@anthropic.com>
Copy file name to clipboardExpand all lines: docs/workflow/demultiplexing.md
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@@ -76,16 +76,27 @@ Create a config file with demux enabled:
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## Barcode Kits
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### Available Kits
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[WarpDemuX](https://github.com/KleistLab/WarpDemuX) provides adapter-based barcode demultiplexing for Oxford Nanopore direct RNA sequencing. This pipeline uses tRNA-specific WarpDemuX models trained for the Nano-tRNAseq protocol.
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### Naming Convention
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Model names follow the format: `WDX[n_barcodes][alt_set]_tRNA_rna004_v1_0`
|`WDX4b_tRNA_rna004_v1_0`| 4 | barcode04, barcode05, barcode07, barcode11 | Alternative adapter set |
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`WDX4_tRNA_rna004_v1_0` provides +3-7% improved read recovery compared to `WDX4b_tRNA_rna004_v1_0`.
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!!! info "Standard RNA004 Models"
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WarpDemuX also offers standard RNA004 models (WDX4, WDX6, WDX10) for mRNA and other direct RNA applications. See the [WarpDemuX README](https://github.com/KleistLab/WarpDemuX) for details. This pipeline requires the **`_tRNA_`** variants.
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!!! warning "Protocol Compatibility"
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WarpDemuX-tRNA models are developed specifically for the **Nano-tRNAseq protocol**. They do **NOT** work with data using the Thomas splint adapter.
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