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This program finds snp sites from a multi fasta alignment file.
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-m output a multi fasta alignment file (default)
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-v output a VCF file
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-p output a phylip file
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-o specify an output filename
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-h this help message
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-m output a multi fasta alignment file (default)
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-v output a VCF file
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-p output a phylip file
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-o specify an output filename
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-h this help message
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<file> input alignment file which can optionally be gzipped
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```
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This application takes in a multi fasta alignment, finds all the SNP sites, then outputs the SNP sites in the following formats:
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a multi fasta alignment,
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VCF,
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relaxed phylip format.
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-a multi fasta alignment,
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-VCF,
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-relaxed phylip format.
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Example usage:
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### Example usage
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```
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snp-sites my_alignment.aln
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snp-sites my_gzipped_alignment.aln.gz
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```
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Multi Fasta Alignment
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=====
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### Multi Fasta Alignment
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Similar to the input file but just containing the SNP sites.
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VCF
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=====
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### VCF
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This contains the position of each SNP in the reference sequence, and the occurrence in each other sample. Can be loaded into Artemis for visualisation.
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Relaxed Phylip format
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=====
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### Relaxed Phylip format
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All the SNP sites in a format for RAxML and other tree building applications.
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