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NEWS.md

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# Unreleased
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# Seurat 5.3.1
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## Changes
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- Added `label.size.cutoff` parameter to `DimPlot` to allow users to label only clusters above a certain size
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### Additions
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- Added option to use `cluster_leiden` from `igraph` when running Leiden clustering ([#9931](https://github.com/satijalab/seurat/pull/9931))
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- Added option to use `umap2` from `uwot` when running UMAP ([#9918](https://github.com/satijalab/seurat/pull/9918))
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- Updated `PseudobulkExpression` to acccount for the case that a category passed to `group.by` has only one value
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- Updated `VlnPlot` to use assay passed to the function if specified
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- Updated `FeatureScatter` to allow gene names with parentheses to be plotted
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- Updated `SingleRasterMap` to store object metadata correctly
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- Made minor edit to description of section running `DoHeatmap` in PBMC3K guided tutorial vignette
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- Updated `FindClusters` to order cluster factor levels correctly when assigning a custom cluster name
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- Updated `FindConservedMarkers` to remove NA values
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- Updated visualization functions to avoid `ggplot2::guides()` deprecation warnings
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- Added option to use `approx_pow` parameter from `uwot` when running UMAP ([#9449](https://github.com/satijalab/seurat/pull/9449))
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- Updated `LoadXenium` to add support for Xenium protein data ([#10024](https://github.com/satijalab/seurat/pull/10024))
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- Added option to specify stroke size in `FeaturePlot` ([#10053](https://github.com/satijalab/seurat/pull/10053))
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- Added `label.size.cutoff` parameter to `DimPlot` to allow users to label only clusters above a certain size ([#10092](https://github.com/satijalab/seurat/pull/10092))
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### Fixes
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- Fixed deprecated calls to Seurat functions `GetAssayData` and `PackageCheck` ([#9924](https://github.com/satijalab/seurat/pull/9924)), ([#10102](https://github.com/satijalab/seurat/pull/10102))
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- Fixed errors in `RunAzimuth` relating to `FindTransferAnchors` ([#9924](https://github.com/satijalab/seurat/pull/9924))
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- Fixed errors in `LeverageScore` relating to handling of (variable) features ([#9924](https://github.com/satijalab/seurat/pull/9924))
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- Updated `PseudobulkExpression` to acccount for the case that a category passed to `group.by` has only one value ([#10092](https://github.com/satijalab/seurat/pull/10092))
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- Updated `VlnPlot` to use assay passed to the function if specified ([#10092](https://github.com/satijalab/seurat/pull/10092))
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- Updated `FeatureScatter` to allow gene names with parentheses to be plotted ([#10092](https://github.com/satijalab/seurat/pull/10092))
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- Updated `SingleRasterMap` to store object metadata correctly ([#10092](https://github.com/satijalab/seurat/pull/10092))
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- Updated `FindClusters` to order cluster factor levels correctly when assigning a custom cluster name ([#10092](https://github.com/satijalab/seurat/pull/10092))
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- Updated `FindConservedMarkers` to remove NA values ([#9917](https://github.com/satijalab/seurat/pull/9917))
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- Updated visualization functions to avoid ggplot2 `guides`, `aes_string`, and `facet_grid` deprecation warnings ([#9409](https://github.com/satijalab/seurat/pull/9409), [#10116](https://github.com/satijalab/seurat/pull/10116))
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- Fixed `DimPlot` bug where metadata columns named 'PC_1', 'UMAP_1' etc override reduction embeddings ([#10062](https://github.com/satijalab/seurat/pull/10062))
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# Seurat 5.3.0
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# Seurat 5.3.0 (2025-04-23)
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## Changes
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- Fixed `PseudobulkExpression` to forward relevant arguments to `NormalizeData` ([#9840](https://github.com/satijalab/seurat/pull/9840))

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