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33<a href =" https://zenodo.org/badge/latestdoi/611932244 " ><img src =" https://zenodo.org/badge/611932244.svg " alt =" DOI " ></a >
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5+ Table of contents:
6+ * [ Introduction] ( #introduction )
7+ * [ Getting started] ( #getting-started )
8+ * [ Usage examples] ( #usage-examples )
9+ * [ GEX] ( #gex )
10+ * [ VDJ] ( #vdj )
11+ * [ ADT] ( #adt )
12+ * [ Running parallel jobs with rush] ( #running-parallel-jobs-with-rush )
13+
14+ ## Introduction
15+
516Here is an R script ` saturation.R ` for estimating sequencing saturation from a
617GEX, VDJ, or ADT dataset from the 10x Genomics platform.
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@@ -114,6 +125,7 @@ Then make a list of input files and pass it to rush:
114125``` bash
115126ls /project/cellranger_output/* /{molecule_info.h5,all_contig_annotations.csv,* .stat.csv.gz} > files.txt
116127
128+ # Run 16 jobs in parallel, capture outputs from each job in one file
117129rush -i files.txt -o rush-saturation.txt -j16 ' Rscript saturation.R --out out/{/%} --file {}'
118130```
119131
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