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Configuration/limits.txt: changing dos to unix line ends for issue #38 requested by @hemant-goyal
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Configuration/limits.txt

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# For each of the modules you can choose to not run that
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# module at all by setting the value below to 1 for the
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# modules you want to remove.
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duplication ignore 0
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kmer ignore 1
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n_content ignore 0
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overrepresented ignore 0
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quality_base ignore 0
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sequence ignore 0
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gc_sequence ignore 0
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quality_sequence ignore 0
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tile ignore 0
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sequence_length ignore 0
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adapter ignore 0
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# For the duplication module the value is the percentage
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# remaining after deduplication. Measured levels below
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# these limits trigger the warning / error.
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duplication warn 70
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duplication error 50
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# For the kmer module the filter is on the -log10 binomial
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# pvalue for the most significant Kmer, so 5 would be
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# 10^-5 = p<0.00001
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kmer warn 2
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kmer error 5
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# For the N module the filter is on the percentage of Ns
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# at any position in the library
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n_content warn 5
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n_content error 20
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# For the overrepresented seqs the warn value sets the
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# threshold for the overrepresented sequences to be reported
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# at all as the proportion of the library which must be seen
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# as a single sequence
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overrepresented warn 0.1
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overrepresented error 1
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# The per base quality filter uses two values, one for the value
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# of the lower quartile, and the other for the value of the
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# median quality. Failing either of these will trigger the alert
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quality_base_lower warn 10
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quality_base_lower error 5
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quality_base_median warn 25
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quality_base_median error 20
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# The per base sequence content module tests the maximum deviation
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# between A and T or C and G
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sequence warn 10
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sequence error 20
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# The per sequence GC content tests the maximum deviation between
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# the theoretical distribution and the real distribution
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gc_sequence warn 15
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gc_sequence error 30
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# The per sequence quality module tests the phred score which is
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# most frequently observed
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quality_sequence warn 27
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quality_sequence error 20
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# The per tile module tests the maximum phred score loss between
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# and individual tile and the average for that base across all tiles
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tile warn 5
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tile error 10
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# The sequence length module tests are binary, so the values here
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# simply turn them on or off. The actual tests warn if you have
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# sequences of different length, and error if you have sequences
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# of zero length.
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sequence_length warn 1
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sequence_length error 1
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# The adapter module's warnings and errors are based on the
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# percentage of reads in the library which have been observed
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# to contain an adapter associated Kmer at any point
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adapter warn 5
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adapter error 10
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# For each of the modules you can choose to not run that
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# module at all by setting the value below to 1 for the
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# modules you want to remove.
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duplication ignore 0
5+
kmer ignore 1
6+
n_content ignore 0
7+
overrepresented ignore 0
8+
quality_base ignore 0
9+
sequence ignore 0
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gc_sequence ignore 0
11+
quality_sequence ignore 0
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tile ignore 0
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sequence_length ignore 0
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adapter ignore 0
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# For the duplication module the value is the percentage
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# remaining after deduplication. Measured levels below
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# these limits trigger the warning / error.
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duplication warn 70
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duplication error 50
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# For the kmer module the filter is on the -log10 binomial
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# pvalue for the most significant Kmer, so 5 would be
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# 10^-5 = p<0.00001
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kmer warn 2
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kmer error 5
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# For the N module the filter is on the percentage of Ns
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# at any position in the library
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n_content warn 5
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n_content error 20
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# For the overrepresented seqs the warn value sets the
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# threshold for the overrepresented sequences to be reported
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# at all as the proportion of the library which must be seen
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# as a single sequence
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overrepresented warn 0.1
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overrepresented error 1
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# The per base quality filter uses two values, one for the value
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# of the lower quartile, and the other for the value of the
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# median quality. Failing either of these will trigger the alert
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quality_base_lower warn 10
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quality_base_lower error 5
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quality_base_median warn 25
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quality_base_median error 20
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# The per base sequence content module tests the maximum deviation
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# between A and T or C and G
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sequence warn 10
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sequence error 20
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# The per sequence GC content tests the maximum deviation between
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# the theoretical distribution and the real distribution
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gc_sequence warn 15
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gc_sequence error 30
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# The per sequence quality module tests the phred score which is
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# most frequently observed
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quality_sequence warn 27
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quality_sequence error 20
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# The per tile module tests the maximum phred score loss between
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# and individual tile and the average for that base across all tiles
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tile warn 5
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tile error 10
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# The sequence length module tests are binary, so the values here
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# simply turn them on or off. The actual tests warn if you have
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# sequences of different length, and error if you have sequences
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# of zero length.
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sequence_length warn 1
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sequence_length error 1
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# The adapter module's warnings and errors are based on the
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# percentage of reads in the library which have been observed
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# to contain an adapter associated Kmer at any point
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adapter warn 5
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adapter error 10

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