|
1 | | -# For each of the modules you can choose to not run that |
2 | | -# module at all by setting the value below to 1 for the |
3 | | -# modules you want to remove. |
4 | | -duplication ignore 0 |
5 | | -kmer ignore 1 |
6 | | -n_content ignore 0 |
7 | | -overrepresented ignore 0 |
8 | | -quality_base ignore 0 |
9 | | -sequence ignore 0 |
10 | | -gc_sequence ignore 0 |
11 | | -quality_sequence ignore 0 |
12 | | -tile ignore 0 |
13 | | -sequence_length ignore 0 |
14 | | -adapter ignore 0 |
15 | | -
|
16 | | -# For the duplication module the value is the percentage |
17 | | -# remaining after deduplication. Measured levels below |
18 | | -# these limits trigger the warning / error. |
19 | | -duplication warn 70 |
20 | | -duplication error 50 |
21 | | -
|
22 | | -# For the kmer module the filter is on the -log10 binomial |
23 | | -# pvalue for the most significant Kmer, so 5 would be |
24 | | -# 10^-5 = p<0.00001 |
25 | | -kmer warn 2 |
26 | | -kmer error 5 |
27 | | -
|
28 | | -# For the N module the filter is on the percentage of Ns |
29 | | -# at any position in the library |
30 | | -n_content warn 5 |
31 | | -n_content error 20 |
32 | | -
|
33 | | -# For the overrepresented seqs the warn value sets the |
34 | | -# threshold for the overrepresented sequences to be reported |
35 | | -# at all as the proportion of the library which must be seen |
36 | | -# as a single sequence |
37 | | -overrepresented warn 0.1 |
38 | | -overrepresented error 1 |
39 | | -
|
40 | | -# The per base quality filter uses two values, one for the value |
41 | | -# of the lower quartile, and the other for the value of the |
42 | | -# median quality. Failing either of these will trigger the alert |
43 | | -quality_base_lower warn 10 |
44 | | -quality_base_lower error 5 |
45 | | -quality_base_median warn 25 |
46 | | -quality_base_median error 20 |
47 | | -
|
48 | | -# The per base sequence content module tests the maximum deviation |
49 | | -# between A and T or C and G |
50 | | -sequence warn 10 |
51 | | -sequence error 20 |
52 | | -
|
53 | | -# The per sequence GC content tests the maximum deviation between |
54 | | -# the theoretical distribution and the real distribution |
55 | | -gc_sequence warn 15 |
56 | | -gc_sequence error 30 |
57 | | -
|
58 | | -# The per sequence quality module tests the phred score which is |
59 | | -# most frequently observed |
60 | | -quality_sequence warn 27 |
61 | | -quality_sequence error 20 |
62 | | -
|
63 | | -# The per tile module tests the maximum phred score loss between |
64 | | -# and individual tile and the average for that base across all tiles |
65 | | -tile warn 5 |
66 | | -tile error 10 |
67 | | -
|
68 | | -# The sequence length module tests are binary, so the values here |
69 | | -# simply turn them on or off. The actual tests warn if you have |
70 | | -# sequences of different length, and error if you have sequences |
71 | | -# of zero length. |
72 | | -
|
73 | | -sequence_length warn 1 |
74 | | -sequence_length error 1 |
75 | | -
|
76 | | -# The adapter module's warnings and errors are based on the |
77 | | -# percentage of reads in the library which have been observed |
78 | | -# to contain an adapter associated Kmer at any point |
79 | | -
|
80 | | -adapter warn 5 |
81 | | -adapter error 10 |
| 1 | +# For each of the modules you can choose to not run that |
| 2 | +# module at all by setting the value below to 1 for the |
| 3 | +# modules you want to remove. |
| 4 | +duplication ignore 0 |
| 5 | +kmer ignore 1 |
| 6 | +n_content ignore 0 |
| 7 | +overrepresented ignore 0 |
| 8 | +quality_base ignore 0 |
| 9 | +sequence ignore 0 |
| 10 | +gc_sequence ignore 0 |
| 11 | +quality_sequence ignore 0 |
| 12 | +tile ignore 0 |
| 13 | +sequence_length ignore 0 |
| 14 | +adapter ignore 0 |
| 15 | + |
| 16 | +# For the duplication module the value is the percentage |
| 17 | +# remaining after deduplication. Measured levels below |
| 18 | +# these limits trigger the warning / error. |
| 19 | +duplication warn 70 |
| 20 | +duplication error 50 |
| 21 | + |
| 22 | +# For the kmer module the filter is on the -log10 binomial |
| 23 | +# pvalue for the most significant Kmer, so 5 would be |
| 24 | +# 10^-5 = p<0.00001 |
| 25 | +kmer warn 2 |
| 26 | +kmer error 5 |
| 27 | + |
| 28 | +# For the N module the filter is on the percentage of Ns |
| 29 | +# at any position in the library |
| 30 | +n_content warn 5 |
| 31 | +n_content error 20 |
| 32 | + |
| 33 | +# For the overrepresented seqs the warn value sets the |
| 34 | +# threshold for the overrepresented sequences to be reported |
| 35 | +# at all as the proportion of the library which must be seen |
| 36 | +# as a single sequence |
| 37 | +overrepresented warn 0.1 |
| 38 | +overrepresented error 1 |
| 39 | + |
| 40 | +# The per base quality filter uses two values, one for the value |
| 41 | +# of the lower quartile, and the other for the value of the |
| 42 | +# median quality. Failing either of these will trigger the alert |
| 43 | +quality_base_lower warn 10 |
| 44 | +quality_base_lower error 5 |
| 45 | +quality_base_median warn 25 |
| 46 | +quality_base_median error 20 |
| 47 | + |
| 48 | +# The per base sequence content module tests the maximum deviation |
| 49 | +# between A and T or C and G |
| 50 | +sequence warn 10 |
| 51 | +sequence error 20 |
| 52 | + |
| 53 | +# The per sequence GC content tests the maximum deviation between |
| 54 | +# the theoretical distribution and the real distribution |
| 55 | +gc_sequence warn 15 |
| 56 | +gc_sequence error 30 |
| 57 | + |
| 58 | +# The per sequence quality module tests the phred score which is |
| 59 | +# most frequently observed |
| 60 | +quality_sequence warn 27 |
| 61 | +quality_sequence error 20 |
| 62 | + |
| 63 | +# The per tile module tests the maximum phred score loss between |
| 64 | +# and individual tile and the average for that base across all tiles |
| 65 | +tile warn 5 |
| 66 | +tile error 10 |
| 67 | + |
| 68 | +# The sequence length module tests are binary, so the values here |
| 69 | +# simply turn them on or off. The actual tests warn if you have |
| 70 | +# sequences of different length, and error if you have sequences |
| 71 | +# of zero length. |
| 72 | + |
| 73 | +sequence_length warn 1 |
| 74 | +sequence_length error 1 |
| 75 | + |
| 76 | +# The adapter module's warnings and errors are based on the |
| 77 | +# percentage of reads in the library which have been observed |
| 78 | +# to contain an adapter associated Kmer at any point |
| 79 | + |
| 80 | +adapter warn 5 |
| 81 | +adapter error 10 |
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