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Merge pull request #2 from SimonWaldherr/patch-1
link to new, recommended DOI resolver in README.md
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report/50-highest-genes.rst

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transcripts suggests that too much spike-in RNA was added during library
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preparation, while the absence of ribosomal proteins and/or the presence of
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their pseudogenes are indicative of suboptimal alignment.
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See `Lun et al. (2016) <http://dx.doi.org/10.12688/f1000research.9501.2>`_.
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See `Lun et al. (2016) <https://doi.org/10.12688/f1000research.9501.2>`_.

report/cycle-scores.rst

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Cells are classified into cycle phases using the method of `Scialdone et al. (2015) <http://dx.doi.org/10.1016/j.ymeth.2015.06.021>`_.
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Cells are classified into cycle phases using the method of `Scialdone et al. (2015) <https://doi.org/10.1016/j.ymeth.2015.06.021>`_.
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This plot shows the scores for G1 and G2/M, each point represents a cell.
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Points are color coded by {{ snakemake.wildcards.condition }}.

report/explained-variance.rst

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log-expression values across cells that is explained by each covariate is
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calculated. Each curve corresponds to one factor and represents the distribution
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of percentages across all genes. See `Lun et al. (2016)
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<http://dx.doi.org/10.12688/f1000research.9501.2>`_.
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<https://doi.org/10.12688/f1000research.9501.2>`_.

report/filtering.rst

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Cells were filtered as suggested by `Lun et al. (2016) <http://dx.doi.org/10.12688/f1000research.9501.2>`_ by removing by removing outliers regarding
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Cells were filtered as suggested by `Lun et al. (2016) <https://doi.org/10.12688/f1000research.9501.2>`_ by removing by removing outliers regarding
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* library size,
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* number of expressed features,

report/hvg-clusters.rst

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in a graph and each pair of features with significantly large
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(:math:`FDR \geq {{snakemake.params.fdr}}`) correlations as an edge.
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Clusters in this graph represent a set of correlated features.
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See `Lun et al. (2016) <http://dx.doi.org/10.12688/f1000research.9501.2>`_.
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See `Lun et al. (2016) <https://doi.org/10.12688/f1000research.9501.2>`_.

report/hvg-corr-heatmap.rst

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(:math:`FDR \geq {{snakemake.params.fdr}}`) correlated HVGs.
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Dendrograms were formed by hierarchical clustering on the Euclidean distances
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between features (rows) or cells (columns).
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See `Lun et al. (2016) <http://dx.doi.org/10.12688/f1000research.9501.2>`_.
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See `Lun et al. (2016) <https://doi.org/10.12688/f1000research.9501.2>`_.

report/hvg-corr-pca.rst

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First, second, and third components are shown, along with the percentage of variance
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explained. Bars represent the coordinates of the cells on each axis.
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Cells were colored by {{ snakemake.wildcards.covariate }}.
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See `Lun et al. (2016) <http://dx.doi.org/10.12688/f1000research.9501.2>`_.
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See `Lun et al. (2016) <https://doi.org/10.12688/f1000research.9501.2>`_.

report/hvg-correlations.rst

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Table of pairs of correlated HVGs.
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Correlations between genes are quantified by computing Spearman's rho, which accommodates non-linear relationships in the expression values.
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See `Lun et al. (2016) <http://dx.doi.org/10.12688/f1000research.9501.2>`_.
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See `Lun et al. (2016) <https://doi.org/10.12688/f1000research.9501.2>`_.

report/hvg.rst

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biological components. This avoids prioritizing genes that are highly variable
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due to technical factors such as sampling noise during RNA capture and library
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preparation. See
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`Lun et al. (2016) <http://dx.doi.org/10.12688/f1000research.9501.2>`_.
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`Lun et al. (2016) <https://doi.org/10.12688/f1000research.9501.2>`_.
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{% if snakemake.params.use_spikes -%}
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Variance was estimated by fitting a mean-variance trend to spike-in transcript

report/mean-vs-variance.rst

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the mean log-expression. The blue line represents the mean-dependent trend
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fitted to the variances of the endogenous genes. Variance estimates for spike-in
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transcripts are highlighted in red.
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See `Lun et al. (2016) <http://dx.doi.org/10.12688/f1000research.9501.2>`_.
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See `Lun et al. (2016) <https://doi.org/10.12688/f1000research.9501.2>`_.

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