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Maybe the answer will be that I should update my software, but I'll report it anyways. When trying to use k=51 signatures against a k=51 database, the fastgather script says they are not compatible:
== This is sourmash version 4.8.11. ==
== Please cite Irber et. al (2024), doi:10.21105/joss.06830. ==
=> sourmash_plugin_branchwater 0.9.5; cite Irber et al., doi: 10.1101/2022.11.02.514947
ksize: 31 / scaled: 1000 / moltype: DNA / threshold bp: 50000
gathering all sketches in '/net/nfs-ip34/home/def-ilafores/analysis/PROVID-LD/fullSet/data/composition/signatures_k51/326_J30.sig' against '/net/nfs-ip34/home/def-ilafores/ref_dbs/sourmash_db/ncbi-euks-fungi-2025.01.dna.k51.sig.zip' using 48 threads
Reading query(s) from: '/net/nfs-ip34/home/def-ilafores/analysis/PROVID-LD/fullSet/data/composition/signatures_k51/326_J30.sig'
WARNING: skipped 1 query paths - no compatible signatures.
Error: No query signatures loaded, exiting.
vs normal gather:
== This is sourmash version 4.8.11. ==
== Please cite Irber et. al (2024), doi:10.21105/joss.06830. ==
select query k=51 automatically.
loaded query: 326_J30... (k=51, DNA)
--
loaded 6260 total signatures from 1 locations.
after selecting signatures compatible with search, 6260 remain.
Starting prefetch sweep across databases.
Prefetch found 2 signatures with overlap >= 50.0 kbp.
Doing gather to generate minimum metagenome cover.
overlap p_query p_match avg_abund
--------- ------- ------- ---------
80.0 kbp 0.0% 0.2% 5.1 GCA_028456235.1 Ophiosphaerella narmari
found less than 50.0 kbp in common. => exiting
found 1 matches total;
the recovered matches hit 0.0% of the abundance-weighted query.
the recovered matches hit 0.0% of the query k-mers (unweighted).
WARNING: final scaled was 10000, vs query scaled of 1000
my metagenomes have a scaling of 1000 and my db , 10000, but that doesnt prevent gather from working.
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