For Users: CLI

The CLI version deploys a command called primertool.

Running modes

Full mode

$ primertool full -h

In this mode, primertool accepts target regions or target sequences (called query) defined by users, designs primers from these regions, and searches user defined databases (called templates) to defined primer specificities.

Design mode

$ primertool design -h

In this mode, primertool just designs primers and doesn't conduct specificity check. This is usually used in NGS target sequencing panels where primer specificity is not so necessary as in legacy PCR.

Checking mode

$ primertool check -h

In this mode, primertool accepts a set of user defined primer sequences (called query) and check their specificity against certain databases (called templates).