diff --git a/packages/vite/src/node/plugins/workerImportMetaUrl.ts b/packages/vite/src/node/plugins/workerImportMetaUrl.ts
index 2f9706540b8c93..7c5af7a1bdeaaa 100644
--- a/packages/vite/src/node/plugins/workerImportMetaUrl.ts
+++ b/packages/vite/src/node/plugins/workerImportMetaUrl.ts
@@ -184,6 +184,94 @@ async function getWorkerType(
 const workerImportMetaUrlRE =
   /new\s+(?:Worker|SharedWorker)\s*\(\s*new\s+URL.+?import\.meta\.url/s
 
+/**
+ * Checks if a template literal only contains safe expressions (import.meta.env.*)
+ * and transforms it to string concatenation if safe.
+ * Returns null if the template contains unsafe dynamic expressions.
+ */
+async function transformSafeTemplateLiteral(
+  rawUrl: string,
+): Promise<string | null> {
+  // Not a template literal
+  if (rawUrl[0] !== '`' || !rawUrl.includes('${')) {
+    return null
+  }
+
+  try {
+    // Parse the template literal as an expression
+    const ast = await parseAstAsync(`(${rawUrl})`)
+    const expression = (ast.body[0] as RollupAstNode<ExpressionStatement>)
+      .expression
+
+    if (expression.type !== 'TemplateLiteral') {
+      return null
+    }
+
+    // Check if all expressions are safe (import.meta.env.*)
+    for (const expr of expression.expressions) {
+      if (!isSafeEnvExpression(expr)) {
+        return null
+      }
+    }
+
+    // Transform to string concatenation
+    const parts: string[] = []
+    for (let i = 0; i < expression.quasis.length; i++) {
+      const quasi = expression.quasis[i]
+      const quasiValue = quasi.value.raw
+
+      if (quasiValue) {
+        parts.push(JSON.stringify(quasiValue))
+      }
+
+      if (i < expression.expressions.length) {
+        const expr = expression.expressions[i]
+        parts.push(generateEnvAccessCode(expr))
+      }
+    }
+
+    return parts.join(' + ')
+  } catch {
+    // If parsing fails, treat as unsafe
+    return null
+  }
+}
+
+/**
+ * Checks if an expression is a safe import.meta.env.* access
+ */
+function isSafeEnvExpression(expr: any): boolean {
+  if (expr.type !== 'MemberExpression') {
+    return false
+  }
+
+  // Check if it's import.meta.env.*
+  if (
+    expr.object.type === 'MemberExpression' &&
+    expr.object.object.type === 'MetaProperty' &&
+    expr.object.object.meta.name === 'import' &&
+    expr.object.object.property.name === 'meta' &&
+    expr.object.property.type === 'Identifier' &&
+    expr.object.property.name === 'env'
+  ) {
+    return true
+  }
+
+  return false
+}
+
+/**
+ * Generates code for accessing import.meta.env property
+ */
+function generateEnvAccessCode(expr: any): string {
+  if (expr.property.type === 'Identifier') {
+    return `import.meta.env.${expr.property.name}`
+  } else if (expr.property.type === 'Literal') {
+    return `import.meta.env[${JSON.stringify(expr.property.value)}]`
+  }
+  return 'import.meta.env'
+}
+
 export function workerImportMetaUrlPlugin(config: ResolvedConfig): Plugin {
   const isBuild = config.command === 'build'
   let workerResolver: ResolveIdFn
@@ -209,6 +297,42 @@ export function workerImportMetaUrlPlugin(config: ResolvedConfig): Plugin {
       async handler(code, id) {
         let s: MagicString | undefined
         const cleanString = stripLiteral(code)
+
+        // First, check if there are template literals with expressions to transform
+        const templateLiteralRE =
+          /\bnew\s+(?:Worker|SharedWorker)\s*\(\s*new\s+URL\s*\(\s*(`[^`]+`)\s*,\s*import\.meta\.url\s*\)/dg
+
+        let templateMatch: RegExpExecArray | null
+        let hasTransformedTemplates = false
+
+        while ((templateMatch = templateLiteralRE.exec(cleanString))) {
+          const [[,], [urlStart, urlEnd]] = templateMatch.indices!
+          const rawUrl = code.slice(urlStart, urlEnd)
+
+          if (rawUrl.includes('${')) {
+            const transformed = await transformSafeTemplateLiteral(rawUrl)
+            if (transformed) {
+              s ||= new MagicString(code)
+              s.update(urlStart, urlEnd, transformed)
+              hasTransformedTemplates = true
+            } else {
+              // Unsafe dynamic template string
+              this.error(
+                `\`new URL(url, import.meta.url)\` is not supported in dynamic template string.\n` +
+                  `Only template literals with \`import.meta.env.*\` expressions are supported.\n` +
+                  `Use string concatenation instead: new URL('path/' + variable + '/file.ts', import.meta.url)`,
+                urlStart,
+              )
+            }
+          }
+        }
+
+        // If we transformed templates, return and let this run again
+        if (hasTransformedTemplates && s) {
+          return transformStableResult(s, id, config)
+        }
+
+        // Process worker URLs (regular strings and template literals without expressions)
         const workerImportMetaUrlRE =
           /\bnew\s+(?:Worker|SharedWorker)\s*\(\s*(new\s+URL\s*\(\s*('[^']+'|"[^"]+"|`[^`]+`)\s*,\s*import\.meta\.url\s*\))/dg
 
@@ -219,12 +343,9 @@ export function workerImportMetaUrlPlugin(config: ResolvedConfig): Plugin {
 
           const rawUrl = code.slice(urlStart, urlEnd)
 
-          // potential dynamic template string
+          // Skip template literals with expressions (should not happen at this point)
           if (rawUrl[0] === '`' && rawUrl.includes('${')) {
-            this.error(
-              `\`new URL(url, import.meta.url)\` is not supported in dynamic template string.`,
-              expStart,
-            )
+            continue
           }
 
           s ||= new MagicString(code)
diff --git a/playground/worker/.env.example b/playground/worker/.env.example
new file mode 100644
index 00000000000000..277a0e70b4550c
--- /dev/null
+++ b/playground/worker/.env.example
@@ -0,0 +1,12 @@
+# Example environment variables for worker template literal tests
+# Copy this file to .env and uncomment to test the template literal feature
+
+# Directory where workers are located
+# Example: VITE_WORKER_DIR=worker
+# Results in path: ./worker/simple-worker.js
+VITE_WORKER_DIR=worker
+
+# Worker file name for testing multiple env vars
+# Example: VITE_WORKER_FILE=simple-worker
+# Combined with VITE_WORKER_DIR results in: ./worker/simple-worker.js
+VITE_WORKER_FILE=simple-worker
diff --git a/playground/worker/env-path-worker.ts b/playground/worker/env-path-worker.ts
new file mode 100644
index 00000000000000..8f58cc77bf7c54
--- /dev/null
+++ b/playground/worker/env-path-worker.ts
@@ -0,0 +1,5 @@
+// Worker for testing template literal paths with import.meta.env
+self.onmessage = (e) => {
+  console.log('env-path-worker received:', e.data)
+  self.postMessage({ pong: 'from-env-path-worker' })
+}
diff --git a/playground/worker/template-literal-test.html b/playground/worker/template-literal-test.html
new file mode 100644
index 00000000000000..b93e4ec1c49a80
--- /dev/null
+++ b/playground/worker/template-literal-test.html
@@ -0,0 +1,126 @@
+<!doctype html>
+<html>
+  <head>
+    <title>Template Literal Worker Test</title>
+  </head>
+  <body>
+    <h1>Template Literal Worker Path Test</h1>
+    <p>
+      This test demonstrates template literals with import.meta.env in worker
+      URLs.
+    </p>
+    <p>
+      Set <code>VITE_WORKER_DIR=worker</code> in .env file to test (defaults to
+      empty string if not set).
+    </p>
+
+    <div>
+      <p>
+        Test 1 - Single env variable in template (transformed to string
+        concatenation):
+      </p>
+      <pre><code>new URL(`./\${import.meta.env.VITE_WORKER_DIR}/simple-worker.js`, import.meta.url)</code></pre>
+      <div id="test1-result">Waiting...</div>
+    </div>
+
+    <div>
+      <p>
+        Test 2 - Multiple env variables (demonstrating complex template
+        literal):
+      </p>
+      <pre><code>new URL(`./\${import.meta.env.VITE_WORKER_DIR}/\${import.meta.env.VITE_WORKER_FILE}.js`, import.meta.url)</code></pre>
+      <div id="test2-result">Waiting...</div>
+    </div>
+
+    <div>
+      <p>Test 3 - Fallback to simple path when env not set:</p>
+      <div id="test3-result">Waiting...</div>
+    </div>
+
+    <script type="module">
+      // Note: In a real project, set these in your .env file:
+      // VITE_WORKER_DIR=worker
+      // VITE_WORKER_FILE=simple-worker
+
+      // Test 1: Single env variable (transformed to string concatenation)
+      // This template literal: `./${import.meta.env.VITE_WORKER_DIR}/simple-worker.js`
+      // Will be transformed to: './' + import.meta.env.VITE_WORKER_DIR + '/simple-worker.js'
+      try {
+        const worker1 = new Worker(
+          new URL(
+            `./${import.meta.env.VITE_WORKER_DIR}/simple-worker.js`,
+            import.meta.url,
+          ),
+          { type: 'module' },
+        )
+
+        worker1.onmessage = (e) => {
+          document.getElementById('test1-result').textContent =
+            `✅ Success: ${JSON.stringify(e.data)}`
+        }
+
+        worker1.onerror = (e) => {
+          document.getElementById('test1-result').textContent =
+            `❌ Error: ${e.message}`
+        }
+
+        worker1.postMessage('ping from test1')
+      } catch (e) {
+        document.getElementById('test1-result').textContent =
+          `❌ Exception: ${e.message}`
+      }
+
+      // Test 2: Multiple env variables
+      // This demonstrates the feature with multiple import.meta.env expressions
+      // Template: `./${import.meta.env.VITE_WORKER_DIR}/${import.meta.env.VITE_WORKER_FILE}.js`
+      // Will be transformed to: './' + import.meta.env.VITE_WORKER_DIR + '/' + import.meta.env.VITE_WORKER_FILE + '.js'
+      try {
+        const worker2 = new Worker(
+          new URL(
+            `./${import.meta.env.VITE_WORKER_DIR}/${import.meta.env.VITE_WORKER_FILE}.js`,
+            import.meta.url,
+          ),
+          { type: 'module' },
+        )
+
+        worker2.onmessage = (e) => {
+          document.getElementById('test2-result').textContent =
+            `✅ Success: ${JSON.stringify(e.data)}`
+        }
+
+        worker2.onerror = (e) => {
+          document.getElementById('test2-result').textContent =
+            `❌ Error: ${e.message}`
+        }
+
+        worker2.postMessage('ping from test2')
+      } catch (e) {
+        document.getElementById('test2-result').textContent =
+          `❌ Exception: ${e.message}`
+      }
+
+      // Test 3: Regular string (should still work as before)
+      try {
+        const worker3 = new Worker(
+          new URL('./simple-worker.js', import.meta.url),
+          { type: 'module' },
+        )
+
+        worker3.onmessage = (e) => {
+          document.getElementById('test3-result').textContent =
+            `✅ Success: ${JSON.stringify(e.data)}`
+        }
+
+        worker3.onerror = (e) => {
+          document.getElementById('test3-result').textContent =
+            `❌ Error: ${e.message}`
+        }
+
+        worker3.postMessage('ping from test3')
+      } catch (e) {
+        document.getElementById('test3-result').textContent =
+          `❌ Exception: ${e.message}`
+      }
+    </script>
+  </body>
+</html>