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| 1 | +{ |
| 2 | + "cells": [ |
| 3 | + { |
| 4 | + "cell_type": "markdown", |
| 5 | + "metadata": { |
| 6 | + "nbsphinx": "hidden" |
| 7 | + }, |
| 8 | + "source": [ |
| 9 | + "# Vitessce Widget Tutorial" |
| 10 | + ] |
| 11 | + }, |
| 12 | + { |
| 13 | + "cell_type": "markdown", |
| 14 | + "metadata": {}, |
| 15 | + "source": [ |
| 16 | + "# Visualization of a SpatialData object\n", |
| 17 | + "\n", |
| 18 | + "Data from https://github.com/scverse/spatialdata-notebooks/blob/main/notebooks/examples/transformations.ipynb" |
| 19 | + ] |
| 20 | + }, |
| 21 | + { |
| 22 | + "cell_type": "markdown", |
| 23 | + "metadata": { |
| 24 | + "tags": [] |
| 25 | + }, |
| 26 | + "source": [ |
| 27 | + "## Import dependencies" |
| 28 | + ] |
| 29 | + }, |
| 30 | + { |
| 31 | + "cell_type": "code", |
| 32 | + "execution_count": null, |
| 33 | + "metadata": {}, |
| 34 | + "outputs": [], |
| 35 | + "source": [ |
| 36 | + "import os\n", |
| 37 | + "from os.path import join, isfile, isdir\n", |
| 38 | + "from urllib.request import urlretrieve\n", |
| 39 | + "import zipfile\n", |
| 40 | + "\n", |
| 41 | + "from vitessce import (\n", |
| 42 | + " VitessceConfig,\n", |
| 43 | + " ViewType as vt,\n", |
| 44 | + " CoordinationType as ct,\n", |
| 45 | + " CoordinationLevel as CL,\n", |
| 46 | + " SpatialDataWrapper,\n", |
| 47 | + " get_initial_coordination_scope_prefix\n", |
| 48 | + ")" |
| 49 | + ] |
| 50 | + }, |
| 51 | + { |
| 52 | + "cell_type": "code", |
| 53 | + "execution_count": null, |
| 54 | + "metadata": {}, |
| 55 | + "outputs": [], |
| 56 | + "source": [ |
| 57 | + "data_dir = \"data\"\n", |
| 58 | + "zip_filepath = join(data_dir, \"mouse_liver.spatialdata.zarr.zip\")\n", |
| 59 | + "spatialdata_filepath = join(data_dir, \"mouse_liver.spatialdata.zarr\")" |
| 60 | + ] |
| 61 | + }, |
| 62 | + { |
| 63 | + "cell_type": "code", |
| 64 | + "execution_count": null, |
| 65 | + "metadata": {}, |
| 66 | + "outputs": [], |
| 67 | + "source": [ |
| 68 | + "if not isdir(spatialdata_filepath):\n", |
| 69 | + " if not isfile(zip_filepath):\n", |
| 70 | + " os.makedirs(data_dir, exist_ok=True)\n", |
| 71 | + " urlretrieve('https://s3.embl.de/spatialdata/spatialdata-sandbox/mouse_liver.zip', zip_filepath)\n", |
| 72 | + " with zipfile.ZipFile(zip_filepath,\"r\") as zip_ref:\n", |
| 73 | + " zip_ref.extractall(data_dir)\n", |
| 74 | + " os.rename(join(data_dir, \"data.zarr\"), spatialdata_filepath)" |
| 75 | + ] |
| 76 | + }, |
| 77 | + { |
| 78 | + "cell_type": "markdown", |
| 79 | + "metadata": {}, |
| 80 | + "source": [ |
| 81 | + "## Configure Vitessce\n", |
| 82 | + "\n", |
| 83 | + "Vitessce needs to know which pieces of data we are interested in visualizing, the visualization types we would like to use, and how we want to coordinate (or link) the views." |
| 84 | + ] |
| 85 | + }, |
| 86 | + { |
| 87 | + "cell_type": "code", |
| 88 | + "execution_count": null, |
| 89 | + "metadata": {}, |
| 90 | + "outputs": [], |
| 91 | + "source": [ |
| 92 | + "vc = VitessceConfig(\n", |
| 93 | + " schema_version=\"1.0.17\",\n", |
| 94 | + " name='SpatialData Demo (mouse_liver)',\n", |
| 95 | + ")\n", |
| 96 | + "# Add data to the configuration:\n", |
| 97 | + "wrapper = SpatialDataWrapper(\n", |
| 98 | + " sdata_store=spatialdata_filepath,\n", |
| 99 | + " # The following paths are relative to the root of the SpatialData zarr store on-disk.\n", |
| 100 | + " table_path=\"tables/table\",\n", |
| 101 | + " image_path=\"images/raw_image\",\n", |
| 102 | + " labels_path=\"labels/segmentation_mask\",\n", |
| 103 | + " #shapes_path=\"shapes/nucleus_boundaries\",\n", |
| 104 | + " #points_path=\"points/transcripts\",\n", |
| 105 | + " obs_feature_matrix_path=\"tables/table/X\",\n", |
| 106 | + " obs_set_paths=[\"tables/table/obs/annotation\"],\n", |
| 107 | + " obs_set_names=[\"Annotation\"],\n", |
| 108 | + " region=\"nucleus_boundaries\",\n", |
| 109 | + " coordinate_system=\"global\",\n", |
| 110 | + " coordination_values={\n", |
| 111 | + " \"obsType\": \"cell\" \n", |
| 112 | + " }\n", |
| 113 | + ")\n", |
| 114 | + "dataset = vc.add_dataset(name='Mouse Liver').add_object(wrapper)\n", |
| 115 | + "\n", |
| 116 | + "# Add views (visualizations) to the configuration:\n", |
| 117 | + "spatial = vc.add_view(\"spatialBeta\", dataset=dataset)\n", |
| 118 | + "feature_list = vc.add_view(vt.FEATURE_LIST, dataset=dataset)\n", |
| 119 | + "layer_controller = vc.add_view(\"layerControllerBeta\", dataset=dataset)\n", |
| 120 | + "obs_sets = vc.add_view(vt.OBS_SETS, dataset=dataset)\n", |
| 121 | + "\n", |
| 122 | + "vc.link_views_by_dict([spatial, layer_controller], {\n", |
| 123 | + " 'imageLayer': CL([{\n", |
| 124 | + " 'photometricInterpretation': 'BlackIsZero',\n", |
| 125 | + " 'imageChannel': CL([{\n", |
| 126 | + " 'spatialChannelColor': [255, 255, 255],\n", |
| 127 | + " 'spatialChannelWindow': [0, 4000],\n", |
| 128 | + " }])\n", |
| 129 | + " }]),\n", |
| 130 | + "}, scope_prefix=get_initial_coordination_scope_prefix(\"A\", \"image\"))\n", |
| 131 | + "\n", |
| 132 | + "vc.link_views_by_dict([spatial, layer_controller], {\n", |
| 133 | + " 'segmentationLayer': CL([{\n", |
| 134 | + " 'segmentationChannel': CL([{\n", |
| 135 | + " 'obsColorEncoding': 'cellSetSelection',\n", |
| 136 | + " }]),\n", |
| 137 | + " }]),\n", |
| 138 | + "}, scope_prefix=get_initial_coordination_scope_prefix(\"A\", \"obsSegmentations\"))\n", |
| 139 | + "\n", |
| 140 | + "vc.link_views([spatial, layer_controller, feature_list, obs_sets], ['obsType'], [wrapper.obs_type_label])\n", |
| 141 | + "\n", |
| 142 | + "# Layout the views\n", |
| 143 | + "vc.layout(spatial | (feature_list / layer_controller / obs_sets));" |
| 144 | + ] |
| 145 | + }, |
| 146 | + { |
| 147 | + "cell_type": "markdown", |
| 148 | + "metadata": {}, |
| 149 | + "source": [ |
| 150 | + "### Render the widget" |
| 151 | + ] |
| 152 | + }, |
| 153 | + { |
| 154 | + "cell_type": "code", |
| 155 | + "execution_count": null, |
| 156 | + "metadata": {}, |
| 157 | + "outputs": [], |
| 158 | + "source": [ |
| 159 | + "vw = vc.widget(custom_js_url=\"http://localhost:3001/packages/main/dev/dist/index.js\")\n", |
| 160 | + "vw" |
| 161 | + ] |
| 162 | + }, |
| 163 | + { |
| 164 | + "cell_type": "code", |
| 165 | + "execution_count": null, |
| 166 | + "metadata": {}, |
| 167 | + "outputs": [], |
| 168 | + "source": [] |
| 169 | + } |
| 170 | + ], |
| 171 | + "metadata": { |
| 172 | + "kernelspec": { |
| 173 | + "display_name": "Python 3 (ipykernel)", |
| 174 | + "language": "python", |
| 175 | + "name": "python3" |
| 176 | + }, |
| 177 | + "language_info": { |
| 178 | + "codemirror_mode": { |
| 179 | + "name": "ipython", |
| 180 | + "version": 3 |
| 181 | + }, |
| 182 | + "file_extension": ".py", |
| 183 | + "mimetype": "text/x-python", |
| 184 | + "name": "python", |
| 185 | + "nbconvert_exporter": "python", |
| 186 | + "pygments_lexer": "ipython3", |
| 187 | + "version": "3.10.14" |
| 188 | + } |
| 189 | + }, |
| 190 | + "nbformat": 4, |
| 191 | + "nbformat_minor": 4 |
| 192 | +} |
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