diff --git a/changes/3371.misc.rst b/changes/3371.misc.rst
new file mode 100644
index 0000000000..06655ee402
--- /dev/null
+++ b/changes/3371.misc.rst
@@ -0,0 +1 @@
+Ensure that tests for executable examples are run in a fresh python environment.
\ No newline at end of file
diff --git a/tests/test_examples.py b/tests/test_examples.py
index c97766364b..54af4119e3 100644
--- a/tests/test_examples.py
+++ b/tests/test_examples.py
@@ -72,7 +72,9 @@ def test_scripts_can_run(script_path: Path, tmp_path: Path) -> None:
     # and then test its behavior.
     # This allows the example to be useful to users who don't have Zarr installed, but also testable.
     resave_script(script_path, dest_path)
-    result = subprocess.run(["uv", "run", str(dest_path)], capture_output=True, text=True)
+    result = subprocess.run(
+        ["uv", "run", "--refresh", str(dest_path)], capture_output=True, text=True
+    )
     assert result.returncode == 0, (
         f"Script at {script_path} failed to run. Output: {result.stdout} Error: {result.stderr}"
     )