diff --git a/boards/posix/native_posix/native_posix.dts b/boards/posix/native_posix/native_posix.dts
index 3bd5555088985..3a3605ac23a96 100644
--- a/boards/posix/native_posix/native_posix.dts
+++ b/boards/posix/native_posix/native_posix.dts
@@ -188,7 +188,7 @@
 	};
 
 	can_loopback0: can_loopback0 {
-		status = "disabled";
+		status = "okay";
 		compatible = "zephyr,can-loopback";
 		sjw = <1>;
 		sample-point = <875>;
diff --git a/samples/drivers/can/counter/sample.yaml b/samples/drivers/can/counter/sample.yaml
index fa7177a9f46da..b6ff5158db1a8 100644
--- a/samples/drivers/can/counter/sample.yaml
+++ b/samples/drivers/can/counter/sample.yaml
@@ -5,6 +5,7 @@ tests:
     tags: can
     depends_on: can
     filter: dt_chosen_enabled("zephyr,canbus")
+    platform_exclude: native_posix native_posix_64
     harness: console
     harness_config:
       type: one_line
diff --git a/samples/net/sockets/can/boards/native_posix.overlay b/samples/net/sockets/can/boards/native_posix.overlay
deleted file mode 100644
index 93b1c32d28697..0000000000000
--- a/samples/net/sockets/can/boards/native_posix.overlay
+++ /dev/null
@@ -1,9 +0,0 @@
-/*
- * Copyright (c) 2022 Vestas Wind Systems A/S
- *
- * SPDX-License-Identifier: Apache-2.0
- */
-
-&can_loopback0 {
-	status = "okay";
-};
diff --git a/samples/net/sockets/can/boards/native_posix_64.overlay b/samples/net/sockets/can/boards/native_posix_64.overlay
deleted file mode 100644
index 93b1c32d28697..0000000000000
--- a/samples/net/sockets/can/boards/native_posix_64.overlay
+++ /dev/null
@@ -1,9 +0,0 @@
-/*
- * Copyright (c) 2022 Vestas Wind Systems A/S
- *
- * SPDX-License-Identifier: Apache-2.0
- */
-
-&can_loopback0 {
-	status = "okay";
-};
diff --git a/tests/drivers/can/api/boards/native_posix.overlay b/tests/drivers/can/api/boards/native_posix.overlay
deleted file mode 100644
index c185f31f6053f..0000000000000
--- a/tests/drivers/can/api/boards/native_posix.overlay
+++ /dev/null
@@ -1,9 +0,0 @@
-/*
- * Copyright (c) 2021 Vestas Wind Systems A/S
- *
- * SPDX-License-Identifier: Apache-2.0
- */
-
-&can_loopback0 {
-	status = "okay";
-};
diff --git a/tests/drivers/can/api/boards/native_posix_64.overlay b/tests/drivers/can/api/boards/native_posix_64.overlay
deleted file mode 100644
index c185f31f6053f..0000000000000
--- a/tests/drivers/can/api/boards/native_posix_64.overlay
+++ /dev/null
@@ -1,9 +0,0 @@
-/*
- * Copyright (c) 2021 Vestas Wind Systems A/S
- *
- * SPDX-License-Identifier: Apache-2.0
- */
-
-&can_loopback0 {
-	status = "okay";
-};
diff --git a/tests/drivers/can/canfd/boards/native_posix.overlay b/tests/drivers/can/canfd/boards/native_posix.overlay
deleted file mode 100644
index 93b1c32d28697..0000000000000
--- a/tests/drivers/can/canfd/boards/native_posix.overlay
+++ /dev/null
@@ -1,9 +0,0 @@
-/*
- * Copyright (c) 2022 Vestas Wind Systems A/S
- *
- * SPDX-License-Identifier: Apache-2.0
- */
-
-&can_loopback0 {
-	status = "okay";
-};
diff --git a/tests/drivers/can/canfd/boards/native_posix_64.overlay b/tests/drivers/can/canfd/boards/native_posix_64.overlay
deleted file mode 100644
index 93b1c32d28697..0000000000000
--- a/tests/drivers/can/canfd/boards/native_posix_64.overlay
+++ /dev/null
@@ -1,9 +0,0 @@
-/*
- * Copyright (c) 2022 Vestas Wind Systems A/S
- *
- * SPDX-License-Identifier: Apache-2.0
- */
-
-&can_loopback0 {
-	status = "okay";
-};
diff --git a/tests/drivers/can/timing/boards/native_posix.overlay b/tests/drivers/can/timing/boards/native_posix.overlay
deleted file mode 100644
index c185f31f6053f..0000000000000
--- a/tests/drivers/can/timing/boards/native_posix.overlay
+++ /dev/null
@@ -1,9 +0,0 @@
-/*
- * Copyright (c) 2021 Vestas Wind Systems A/S
- *
- * SPDX-License-Identifier: Apache-2.0
- */
-
-&can_loopback0 {
-	status = "okay";
-};
diff --git a/tests/drivers/can/timing/boards/native_posix_64.overlay b/tests/drivers/can/timing/boards/native_posix_64.overlay
deleted file mode 100644
index c185f31f6053f..0000000000000
--- a/tests/drivers/can/timing/boards/native_posix_64.overlay
+++ /dev/null
@@ -1,9 +0,0 @@
-/*
- * Copyright (c) 2021 Vestas Wind Systems A/S
- *
- * SPDX-License-Identifier: Apache-2.0
- */
-
-&can_loopback0 {
-	status = "okay";
-};
diff --git a/tests/subsys/canbus/isotp/conformance/testcase.yaml b/tests/subsys/canbus/isotp/conformance/testcase.yaml
index 222b854df31b8..3366607882c56 100644
--- a/tests/subsys/canbus/isotp/conformance/testcase.yaml
+++ b/tests/subsys/canbus/isotp/conformance/testcase.yaml
@@ -3,3 +3,4 @@ tests:
     tags: can isotp
     depends_on: can
     filter: dt_chosen_enabled("zephyr,canbus")
+    platform_exclude: native_posix native_posix_64
diff --git a/tests/subsys/canbus/isotp/implementation/testcase.yaml b/tests/subsys/canbus/isotp/implementation/testcase.yaml
index 1262b76652291..85daa69cacd0f 100644
--- a/tests/subsys/canbus/isotp/implementation/testcase.yaml
+++ b/tests/subsys/canbus/isotp/implementation/testcase.yaml
@@ -3,3 +3,4 @@ tests:
     tags: can isotp
     depends_on: can
     filter: dt_chosen_enabled("zephyr,canbus")
+    platform_exclude: native_posix native_posix_64