diff --git a/tests/bsim/bluetooth/audio/test_scripts/cap_unicast_ac_11_i.sh b/tests/bsim/bluetooth/audio/test_scripts/cap_unicast_ac_11_i.sh
index e74192f47ec8d..3104e42972768 100755
--- a/tests/bsim/bluetooth/audio/test_scripts/cap_unicast_ac_11_i.sh
+++ b/tests/bsim/bluetooth/audio/test_scripts/cap_unicast_ac_11_i.sh
@@ -40,8 +40,8 @@ Execute_AC_11_I 24_1_1 24_1_1
 Execute_AC_11_I 24_2_1 24_2_1
 Execute_AC_11_I 32_1_1 32_1_1
 Execute_AC_11_I 32_2_1 32_2_1
-# Execute_AC_11_I 441_1_1 441_1_1 # ASSERTION FAIL [iso_interval_us >= cig->c_sdu_interval]
-# Execute_AC_11_I 441_2_1 441_2_1 # ASSERTION FAIL [iso_interval_us >= cig->c_sdu_interval]
+Execute_AC_11_I 441_1_1 441_1_1
+Execute_AC_11_I 441_2_1 441_2_1
 Execute_AC_11_I 48_1_1 48_1_1
 Execute_AC_11_I 48_2_1 48_2_1
 Execute_AC_11_I 48_3_1 48_3_1
diff --git a/tests/bsim/bluetooth/audio/test_scripts/cap_unicast_ac_11_ii.sh b/tests/bsim/bluetooth/audio/test_scripts/cap_unicast_ac_11_ii.sh
index 2280172b4ae80..74d6ad8a01158 100755
--- a/tests/bsim/bluetooth/audio/test_scripts/cap_unicast_ac_11_ii.sh
+++ b/tests/bsim/bluetooth/audio/test_scripts/cap_unicast_ac_11_ii.sh
@@ -44,8 +44,8 @@ Execute_AC_11_II 24_1_1 24_1_1
 Execute_AC_11_II 24_2_1 24_2_1
 Execute_AC_11_II 32_1_1 32_1_1
 Execute_AC_11_II 32_2_1 32_2_1
-# Execute_AC_11_II 441_1_1 441_1_1 # ASSERTION FAIL [iso_interval_us >= cig->c_sdu_interval]
-# Execute_AC_11_II 441_2_1 441_2_1 # ASSERTION FAIL [iso_interval_us >= cig->c_sdu_interval]
+Execute_AC_11_II 441_1_1 441_1_1
+Execute_AC_11_II 441_2_1 441_2_1
 Execute_AC_11_II 48_1_1 48_1_1
 Execute_AC_11_II 48_2_1 48_2_1
 Execute_AC_11_II 48_3_1 48_3_1
diff --git a/tests/bsim/bluetooth/audio/test_scripts/cap_unicast_ac_5.sh b/tests/bsim/bluetooth/audio/test_scripts/cap_unicast_ac_5.sh
index d42716febf871..1219a8d5c6416 100755
--- a/tests/bsim/bluetooth/audio/test_scripts/cap_unicast_ac_5.sh
+++ b/tests/bsim/bluetooth/audio/test_scripts/cap_unicast_ac_5.sh
@@ -39,8 +39,8 @@ Execute_AC_5 24_1_1 24_1_1
 Execute_AC_5 24_2_1 24_2_1
 Execute_AC_5 32_1_1 32_1_1
 Execute_AC_5 32_2_1 32_2_1
-# Execute_AC_5 441_1_1 441_1_1 # ASSERTION FAIL [iso_interval_us >= cig->c_sdu_interval]
-# Execute_AC_5 441_2_1 441_2_1 # ASSERTION FAIL [iso_interval_us >= cig->c_sdu_interval]
+Execute_AC_5 441_1_1 441_1_1
+Execute_AC_5 441_2_1 441_2_1
 Execute_AC_5 48_1_1 48_1_1
 Execute_AC_5 48_2_1 48_2_1
 Execute_AC_5 48_3_1 48_3_1
diff --git a/tests/bsim/bluetooth/audio/test_scripts/cap_unicast_ac_6_i.sh b/tests/bsim/bluetooth/audio/test_scripts/cap_unicast_ac_6_i.sh
index 344ebb0448b79..18efed14f7fef 100755
--- a/tests/bsim/bluetooth/audio/test_scripts/cap_unicast_ac_6_i.sh
+++ b/tests/bsim/bluetooth/audio/test_scripts/cap_unicast_ac_6_i.sh
@@ -38,8 +38,8 @@ Execute_AC_6_I 24_1_1
 Execute_AC_6_I 24_2_1
 Execute_AC_6_I 32_1_1
 Execute_AC_6_I 32_2_1
-# Execute_AC_6_I 441_1_1 # ASSERTION FAIL [iso_interval_us >= cig->c_sdu_interval]
-# Execute_AC_6_I 441_2_1 # ASSERTION FAIL [iso_interval_us >= cig->c_sdu_interval]
+Execute_AC_6_I 441_1_1
+Execute_AC_6_I 441_2_1
 Execute_AC_6_I 48_1_1
 Execute_AC_6_I 48_2_1
 Execute_AC_6_I 48_3_1
diff --git a/tests/bsim/bluetooth/audio/test_scripts/cap_unicast_ac_6_ii.sh b/tests/bsim/bluetooth/audio/test_scripts/cap_unicast_ac_6_ii.sh
index e04df69690891..49fea4b5cdfd0 100755
--- a/tests/bsim/bluetooth/audio/test_scripts/cap_unicast_ac_6_ii.sh
+++ b/tests/bsim/bluetooth/audio/test_scripts/cap_unicast_ac_6_ii.sh
@@ -44,8 +44,8 @@ Execute_AC_6_II 24_1_1
 Execute_AC_6_II 24_2_1
 Execute_AC_6_II 32_1_1
 Execute_AC_6_II 32_2_1
-# Execute_AC_6_II 441_1_1 # ASSERTION FAIL [iso_interval_us >= cig->c_sdu_interval]
-# Execute_AC_6_II 441_2_1 # ASSERTION FAIL [iso_interval_us >= cig->c_sdu_interval]
+Execute_AC_6_II 441_1_1
+Execute_AC_6_II 441_2_1
 Execute_AC_6_II 48_1_1
 Execute_AC_6_II 48_2_1
 Execute_AC_6_II 48_3_1
diff --git a/tests/bsim/bluetooth/audio/test_scripts/cap_unicast_ac_7_ii.sh b/tests/bsim/bluetooth/audio/test_scripts/cap_unicast_ac_7_ii.sh
index ce6c9abdf89f8..c2f02b25df8e7 100755
--- a/tests/bsim/bluetooth/audio/test_scripts/cap_unicast_ac_7_ii.sh
+++ b/tests/bsim/bluetooth/audio/test_scripts/cap_unicast_ac_7_ii.sh
@@ -44,8 +44,8 @@ Execute_AC_7_II 24_1_1 24_1_1
 Execute_AC_7_II 24_2_1 24_2_1
 Execute_AC_7_II 32_1_1 32_1_1
 Execute_AC_7_II 32_2_1 32_2_1
-# Execute_AC_7_II 441_1_1 441_1_1 # ASSERTION FAIL [iso_interval_us >= cig->c_sdu_interval]
-# Execute_AC_7_II 441_2_1 441_2_1 # ASSERTION FAIL [iso_interval_us >= cig->c_sdu_interval]
+Execute_AC_7_II 441_1_1 441_1_1
+Execute_AC_7_II 441_2_1 441_2_1
 Execute_AC_7_II 48_1_1 48_1_1
 Execute_AC_7_II 48_2_1 48_2_1
 Execute_AC_7_II 48_3_1 48_3_1
diff --git a/tests/bsim/bluetooth/audio/test_scripts/cap_unicast_ac_8_i.sh b/tests/bsim/bluetooth/audio/test_scripts/cap_unicast_ac_8_i.sh
index 7b1e7d6848101..9b4f67a5470eb 100755
--- a/tests/bsim/bluetooth/audio/test_scripts/cap_unicast_ac_8_i.sh
+++ b/tests/bsim/bluetooth/audio/test_scripts/cap_unicast_ac_8_i.sh
@@ -40,8 +40,8 @@ Execute_AC_8_I 24_1_1 24_1_1
 Execute_AC_8_I 24_2_1 24_2_1
 Execute_AC_8_I 32_1_1 32_1_1
 Execute_AC_8_I 32_2_1 32_2_1
-# Execute_AC_8_I 441_1_1 441_1_1 # ASSERTION FAIL [iso_interval_us >= cig->c_sdu_interval]
-# Execute_AC_8_I 441_2_1 441_2_1 # ASSERTION FAIL [iso_interval_us >= cig->c_sdu_interval]
+Execute_AC_8_I 441_1_1 441_1_1
+Execute_AC_8_I 441_2_1 441_2_1
 Execute_AC_8_I 48_1_1 48_1_1
 Execute_AC_8_I 48_2_1 48_2_1
 Execute_AC_8_I 48_3_1 48_3_1
diff --git a/tests/bsim/bluetooth/audio/test_scripts/cap_unicast_ac_8_ii.sh b/tests/bsim/bluetooth/audio/test_scripts/cap_unicast_ac_8_ii.sh
index 25e07a6c1495b..80df102491088 100755
--- a/tests/bsim/bluetooth/audio/test_scripts/cap_unicast_ac_8_ii.sh
+++ b/tests/bsim/bluetooth/audio/test_scripts/cap_unicast_ac_8_ii.sh
@@ -44,8 +44,8 @@ Execute_AC_8_II 24_1_1 24_1_1
 Execute_AC_8_II 24_2_1 24_2_1
 Execute_AC_8_II 32_1_1 32_1_1
 Execute_AC_8_II 32_2_1 32_2_1
-# Execute_AC_8_II 441_1_1 441_1_1 # ASSERTION FAIL [iso_interval_us >= cig->c_sdu_interval]
-# Execute_AC_8_II 441_2_1 441_2_1 # ASSERTION FAIL [iso_interval_us >= cig->c_sdu_interval]
+Execute_AC_8_II 441_1_1 441_1_1
+Execute_AC_8_II 441_2_1 441_2_1
 Execute_AC_8_II 48_1_1 48_1_1
 Execute_AC_8_II 48_2_1 48_2_1
 Execute_AC_8_II 48_3_1 48_3_1
diff --git a/tests/bsim/bluetooth/audio/test_scripts/cap_unicast_ac_9_i.sh b/tests/bsim/bluetooth/audio/test_scripts/cap_unicast_ac_9_i.sh
index 1a00f55e43307..05b361bc8cafa 100755
--- a/tests/bsim/bluetooth/audio/test_scripts/cap_unicast_ac_9_i.sh
+++ b/tests/bsim/bluetooth/audio/test_scripts/cap_unicast_ac_9_i.sh
@@ -40,8 +40,8 @@ Execute_AC_9_I 24_1_1
 Execute_AC_9_I 24_2_1
 Execute_AC_9_I 32_1_1
 Execute_AC_9_I 32_2_1
-# Execute_AC_9_I 441_1_1 # ASSERTION FAIL [iso_interval_us >= cig->c_sdu_interval]
-# Execute_AC_9_I 441_2_1 # ASSERTION FAIL [iso_interval_us >= cig->c_sdu_interval]
+Execute_AC_9_I 441_1_1
+Execute_AC_9_I 441_2_1
 Execute_AC_9_I 48_1_1
 Execute_AC_9_I 48_2_1
 Execute_AC_9_I 48_3_1
diff --git a/tests/bsim/bluetooth/audio/test_scripts/cap_unicast_ac_9_ii.sh b/tests/bsim/bluetooth/audio/test_scripts/cap_unicast_ac_9_ii.sh
index 207d81f663e74..fb1486f24aba8 100755
--- a/tests/bsim/bluetooth/audio/test_scripts/cap_unicast_ac_9_ii.sh
+++ b/tests/bsim/bluetooth/audio/test_scripts/cap_unicast_ac_9_ii.sh
@@ -44,8 +44,8 @@ Execute_AC_9_II 24_1_1
 Execute_AC_9_II 24_2_1
 Execute_AC_9_II 32_1_1
 Execute_AC_9_II 32_2_1
-# Execute_AC_9_II 441_1_1 # ASSERTION FAIL [iso_interval_us >= cig->c_sdu_interval]
-# Execute_AC_9_II 441_2_1 # ASSERTION FAIL [iso_interval_us >= cig->c_sdu_interval]
+Execute_AC_9_II 441_1_1
+Execute_AC_9_II 441_2_1
 Execute_AC_9_II 48_1_1
 Execute_AC_9_II 48_2_1
 Execute_AC_9_II 48_3_1