diff --git a/arch/riscv/Kconfig b/arch/riscv/Kconfig
index 575c9871c1304..d3d1076b367c5 100644
--- a/arch/riscv/Kconfig
+++ b/arch/riscv/Kconfig
@@ -311,7 +311,6 @@ endif #RISCV_PMP
 
 config PMP_STACK_GUARD
 	def_bool y
-	depends on MULTITHREADING
 	depends on HW_STACK_PROTECTION
 
 config PMP_STACK_GUARD_MIN_SIZE
diff --git a/arch/riscv/core/fatal.c b/arch/riscv/core/fatal.c
index d5cbd2f4dc144..879ffab9a8095 100644
--- a/arch/riscv/core/fatal.c
+++ b/arch/riscv/core/fatal.c
@@ -172,11 +172,21 @@ static bool bad_stack_pointer(struct arch_esf *esf)
 	}
 #endif /* CONFIG_USERSPACE */
 
+#if CONFIG_MULTITHREADING
 	if (sp >= _current->stack_info.start - K_KERNEL_STACK_RESERVED &&
 	    sp <  _current->stack_info.start - K_KERNEL_STACK_RESERVED
 		  + Z_RISCV_STACK_GUARD_SIZE) {
 		return true;
 	}
+#else
+	uintptr_t isr_stack = (uintptr_t)z_interrupt_stacks;
+	uintptr_t main_stack = (uintptr_t)z_main_stack;
+
+	if ((sp >= isr_stack && sp < isr_stack + Z_RISCV_STACK_GUARD_SIZE) ||
+	    (sp >= main_stack && sp < main_stack + Z_RISCV_STACK_GUARD_SIZE)) {
+		return true;
+	}
+#endif /* CONFIG_MULTITHREADING */
 #endif /* CONFIG_PMP_STACK_GUARD */
 
 #ifdef CONFIG_USERSPACE
diff --git a/arch/riscv/core/pmp.c b/arch/riscv/core/pmp.c
index e41eb8d4bb080..e29c8abd76d61 100644
--- a/arch/riscv/core/pmp.c
+++ b/arch/riscv/core/pmp.c
@@ -348,8 +348,8 @@ static unsigned int global_pmp_end_index;
  */
 void z_riscv_pmp_init(void)
 {
-	unsigned long pmp_addr[5];
-	unsigned long pmp_cfg[2];
+	unsigned long pmp_addr[CONFIG_PMP_SLOTS];
+	unsigned long pmp_cfg[CONFIG_PMP_SLOTS / PMPCFG_STRIDE];
 	unsigned int index = 0;
 
 	/* The read-only area is always there for every mode */
@@ -370,6 +370,7 @@ void z_riscv_pmp_init(void)
 #endif
 
 #ifdef CONFIG_PMP_STACK_GUARD
+#ifdef CONFIG_MULTITHREADING
 	/*
 	 * Set the stack guard for this CPU's IRQ stack by making the bottom
 	 * addresses inaccessible. This will never change so we do it here
@@ -396,6 +397,21 @@ void z_riscv_pmp_init(void)
 
 	/* And forget about that last entry as we won't need it later */
 	index--;
+#else
+	/* Without multithreading setup stack guards for IRQ and main stacks */
+	set_pmp_entry(&index, PMP_NONE | PMP_L,
+		      (uintptr_t)z_interrupt_stacks,
+		      Z_RISCV_STACK_GUARD_SIZE,
+		      pmp_addr, pmp_cfg, ARRAY_SIZE(pmp_addr));
+
+	set_pmp_entry(&index, PMP_NONE | PMP_L,
+		      (uintptr_t)z_main_stack,
+		      Z_RISCV_STACK_GUARD_SIZE,
+		      pmp_addr, pmp_cfg, ARRAY_SIZE(pmp_addr));
+
+	/* Write those entries to PMP regs. */
+	write_pmp_entries(0, index, true, pmp_addr, pmp_cfg, ARRAY_SIZE(pmp_addr));
+#endif /* CONFIG_MULTITHREADING */
 #else
 	 /* Write those entries to PMP regs. */
 	write_pmp_entries(0, index, true, pmp_addr, pmp_cfg, ARRAY_SIZE(pmp_addr));
@@ -419,7 +435,6 @@ void z_riscv_pmp_init(void)
 	}
 #endif
 
-	__ASSERT(index <= PMPCFG_STRIDE, "provision for one global word only");
 	global_pmp_cfg[0] = pmp_cfg[0];
 	global_pmp_last_addr = pmp_addr[index - 1];
 	global_pmp_end_index = index;
@@ -454,6 +469,7 @@ static inline unsigned int z_riscv_pmp_thread_init(unsigned long *pmp_addr,
 
 #ifdef CONFIG_PMP_STACK_GUARD
 
+#ifdef CONFIG_MULTITHREADING
 /**
  * @brief Prepare the PMP stackguard content for given thread.
  *
@@ -511,6 +527,8 @@ void z_riscv_pmp_stackguard_enable(struct k_thread *thread)
 	csr_set(mstatus, MSTATUS_MPRV);
 }
 
+#endif /* CONFIG_MULTITHREADING */
+
 /**
  * @brief Remove PMP stackguard content to actual PMP registers
  */
diff --git a/kernel/include/kernel_internal.h b/kernel/include/kernel_internal.h
index bb9ee4b01f1e6..94f90ce94624c 100644
--- a/kernel/include/kernel_internal.h
+++ b/kernel/include/kernel_internal.h
@@ -152,6 +152,7 @@ extern struct k_thread z_idle_threads[CONFIG_MP_MAX_NUM_CPUS];
 #endif /* CONFIG_MULTITHREADING */
 K_KERNEL_PINNED_STACK_ARRAY_DECLARE(z_interrupt_stacks, CONFIG_MP_MAX_NUM_CPUS,
 				    CONFIG_ISR_STACK_SIZE);
+K_THREAD_STACK_DECLARE(z_main_stack, CONFIG_MAIN_STACK_SIZE);
 
 #ifdef CONFIG_GEN_PRIV_STACKS
 extern uint8_t *z_priv_stack_find(k_thread_stack_t *stack);
diff --git a/tests/arch/riscv/pmp/no-multithreading/CMakeLists.txt b/tests/arch/riscv/pmp/no-multithreading/CMakeLists.txt
new file mode 100644
index 0000000000000..e0f392177d6f3
--- /dev/null
+++ b/tests/arch/riscv/pmp/no-multithreading/CMakeLists.txt
@@ -0,0 +1,13 @@
+# SPDX-License-Identifier: Apache-2.0
+
+cmake_minimum_required(VERSION 3.20.0)
+find_package(Zephyr REQUIRED HINTS $ENV{ZEPHYR_BASE})
+project(riscv_pmp)
+
+FILE(GLOB app_sources src/*.c)
+target_sources(app PRIVATE ${app_sources})
+
+target_include_directories(app PRIVATE
+  ${ZEPHYR_BASE}/kernel/include
+  ${ZEPHYR_BASE}/arch/${ARCH}/include
+  )
diff --git a/tests/arch/riscv/pmp/no-multithreading/prj.conf b/tests/arch/riscv/pmp/no-multithreading/prj.conf
new file mode 100644
index 0000000000000..7414c11237a8a
--- /dev/null
+++ b/tests/arch/riscv/pmp/no-multithreading/prj.conf
@@ -0,0 +1,2 @@
+CONFIG_ZTEST=y
+CONFIG_MULTITHREADING=n
diff --git a/tests/arch/riscv/pmp/no-multithreading/src/main.c b/tests/arch/riscv/pmp/no-multithreading/src/main.c
new file mode 100644
index 0000000000000..26ded61165f7a
--- /dev/null
+++ b/tests/arch/riscv/pmp/no-multithreading/src/main.c
@@ -0,0 +1,79 @@
+/*
+ * Copyright (c) 2024 Marvell.
+ * SPDX-License-Identifier: Apache-2.0
+ */
+
+#include <kernel_internal.h>
+#include <zephyr/tc_util.h>
+#include <zephyr/ztest.h>
+
+static volatile ZTEST_BMEM bool valid_fault;
+
+void k_sys_fatal_error_handler(unsigned int reason, const struct arch_esf *pEsf)
+{
+	int rv = TC_PASS;
+
+	TC_PRINT("Caught system error -- reason %d %d\n", reason, valid_fault);
+	if (!valid_fault) {
+		TC_PRINT("Fatal error was unexpected, aborting...\n");
+		rv = TC_FAIL;
+	}
+	TC_END_RESULT_CUSTOM(rv, "test_pmp");
+	TC_END_REPORT(rv);
+	arch_system_halt(reason);
+}
+
+#ifdef CONFIG_PMP_STACK_GUARD
+static void check_isr_stack_guard(void)
+{
+	char *isr_stack = (char *)z_interrupt_stacks;
+
+	valid_fault = true;
+	*isr_stack = 42;
+}
+
+static void check_main_stack_guard(void)
+{
+	char *main_stack = (char *)z_main_stack;
+
+	valid_fault = true;
+	*main_stack = 42;
+}
+
+#else
+
+static void check_isr_stack_guard(void)
+{
+	ztest_test_skip();
+}
+
+static void check_main_stack_guard(void)
+{
+	ztest_test_skip();
+}
+
+#endif /* CONFIG_PMP_STACK_GUARD */
+
+typedef void (*pmp_test_func_t)(void);
+
+static const pmp_test_func_t pmp_test_func[] = {
+	check_isr_stack_guard,
+	check_main_stack_guard,
+};
+
+/**
+ * @brief Verify RISC-V specific PMP stack guard regions.
+ * @details Manually write to the protected stack region to trigger fatal error.
+ */
+ZTEST(riscv_pmp_no_mt, test_pmp)
+{
+#ifndef PMP_TEST_FUNC_IDX
+#define PMP_TEST_FUNC_IDX 0
+#endif
+	pmp_test_func[PMP_TEST_FUNC_IDX]();
+
+	zassert_unreachable("Write to stack guard did not fault");
+	TC_END_REPORT(TC_FAIL);
+}
+
+ZTEST_SUITE(riscv_pmp_no_mt, NULL, NULL, NULL, NULL, NULL);
diff --git a/tests/arch/riscv/pmp/no-multithreading/testcase.yaml b/tests/arch/riscv/pmp/no-multithreading/testcase.yaml
new file mode 100644
index 0000000000000..d8f887fa34121
--- /dev/null
+++ b/tests/arch/riscv/pmp/no-multithreading/testcase.yaml
@@ -0,0 +1,13 @@
+common:
+  platform_allow:
+    - qemu_riscv32
+    - qemu_riscv32e
+    - qemu_riscv64
+  filter: CONFIG_RISCV_PMP
+  ignore_faults: true
+
+tests:
+  arch.riscv.pmp.no-mt.isr-stack-guard:
+    extra_args: EXTRA_CFLAGS=-DPMP_TEST_FUNC_IDX=0
+  arch.riscv.pmp.no-mt.main-stack-guard:
+    extra_args: EXTRA_CFLAGS=-DPMP_TEST_FUNC_IDX=1