README.md

RNA-Seq Analysis Projects in R

A collection of transcriptomics and RNA-Seq analysis projects implemented in R and Bioconductor.

Projects

1. Cardiomyopathy Transcriptomic Analysis

• GEO data integration
• Batch correction using ComBat
• Differential expression analysis using Limma
• PCA, Heatmaps, Volcano plots

2. Time-Course RNA-Seq Analysis using DESeq2 LRT

• Likelihood Ratio Test (LRT)
• Temporal gene expression analysis
• Gene clustering
• Time-course visualization

Tools

• R
• DESeq2
• Limma
• GEOquery
• ggplot2
• pheatmap
• Quarto

Author

Anas Jamshed