diff --git a/ci-operator/config/openshift/machine-config-operator/openshift-machine-config-operator-release-5.0__periodics.yaml b/ci-operator/config/openshift/machine-config-operator/openshift-machine-config-operator-release-5.0__periodics.yaml
index a9e854bd1a57c..f3de26ef4ec8b 100644
--- a/ci-operator/config/openshift/machine-config-operator/openshift-machine-config-operator-release-5.0__periodics.yaml
+++ b/ci-operator/config/openshift/machine-config-operator/openshift-machine-config-operator-release-5.0__periodics.yaml
@@ -155,6 +155,15 @@ tests:
     env:
       TEST_SUITE: openshift/machine-config-operator/disruptive
     workflow: baremetalds-e2e-ovn-dualstack
+- as: e2e-aws-mco-single-node-disruptive
+  cron: 0 0 * * 0
+  steps:
+    cluster_profile: openshift-org-aws
+    env:
+      TEST_SUITE: openshift/machine-config-operator/disruptive
+    test:
+    - ref: openshift-e2e-test-longrun
+    workflow: openshift-e2e-aws-single-node
 - as: e2e-aws-mco-disruptive-techpreview
   interval: 24h
   reporter_config:
@@ -280,6 +289,14 @@ tests:
         FEATURE_SET=TechPreviewNoUpgrade
       TEST_SUITE: openshift/machine-config-operator/disruptive
     workflow: baremetalds-e2e-ovn-dualstack
+- as: e2e-aws-mco-single-node-disruptive-techpreview
+  cron: 0 0 * * 6
+  steps:
+    cluster_profile: openshift-org-aws
+    env:
+      FEATURE_SET: TechPreviewNoUpgrade
+      TEST_SUITE: openshift/machine-config-operator/disruptive
+    workflow: openshift-e2e-aws-single-node
 - as: e2e-aws-ovn-ocl
   interval: 168h
   reporter_config:
diff --git a/ci-operator/jobs/openshift/machine-config-operator/openshift-machine-config-operator-release-5.0-periodics.yaml b/ci-operator/jobs/openshift/machine-config-operator/openshift-machine-config-operator-release-5.0-periodics.yaml
index d3a68545ce7f1..22c08b9656e51 100644
--- a/ci-operator/jobs/openshift/machine-config-operator/openshift-machine-config-operator-release-5.0-periodics.yaml
+++ b/ci-operator/jobs/openshift/machine-config-operator/openshift-machine-config-operator-release-5.0-periodics.yaml
@@ -959,6 +959,182 @@ periodics:
     - name: result-aggregator
       secret:
         secretName: result-aggregator
+- agent: kubernetes
+  cluster: build07
+  cron: 0 0 * * 0
+  decorate: true
+  decoration_config:
+    sparse_checkout_files:
+    - .ci-operator.yaml
+    - Dockerfile.rhel7
+  extra_refs:
+  - base_ref: release-5.0
+    org: openshift
+    repo: machine-config-operator
+    sparse_checkout_files:
+    - .ci-operator.yaml
+    - Dockerfile.rhel7
+  labels:
+    ci-operator.openshift.io/cloud: aws
+    ci-operator.openshift.io/cloud-cluster-profile: openshift-org-aws
+    ci-operator.openshift.io/variant: periodics
+    ci.openshift.io/generator: prowgen
+    job-release: "5.0"
+    pj-rehearse.openshift.io/can-be-rehearsed: "true"
+  name: periodic-ci-openshift-machine-config-operator-release-5.0-periodics-e2e-aws-mco-single-node-disruptive
+  spec:
+    containers:
+    - args:
+      - --gcs-upload-secret=/secrets/gcs/service-account.json
+      - --image-import-pull-secret=/etc/pull-secret/.dockerconfigjson
+      - --lease-server-credentials-file=/etc/boskos/credentials
+      - --report-credentials-file=/etc/report/credentials
+      - --secret-dir=/secrets/ci-pull-credentials
+      - --target=e2e-aws-mco-single-node-disruptive
+      - --variant=periodics
+      command:
+      - ci-operator
+      env:
+      - name: HTTP_SERVER_IP
+        valueFrom:
+          fieldRef:
+            fieldPath: status.podIP
+      image: quay-proxy.ci.openshift.org/openshift/ci:ci_ci-operator_latest
+      imagePullPolicy: Always
+      name: ""
+      ports:
+      - containerPort: 8080
+        name: http
+      resources:
+        requests:
+          cpu: 10m
+      volumeMounts:
+      - mountPath: /etc/boskos
+        name: boskos
+        readOnly: true
+      - mountPath: /secrets/ci-pull-credentials
+        name: ci-pull-credentials
+        readOnly: true
+      - mountPath: /secrets/gcs
+        name: gcs-credentials
+        readOnly: true
+      - mountPath: /secrets/manifest-tool
+        name: manifest-tool-local-pusher
+        readOnly: true
+      - mountPath: /etc/pull-secret
+        name: pull-secret
+        readOnly: true
+      - mountPath: /etc/report
+        name: result-aggregator
+        readOnly: true
+    serviceAccountName: ci-operator
+    volumes:
+    - name: boskos
+      secret:
+        items:
+        - key: credentials
+          path: credentials
+        secretName: boskos-credentials
+    - name: ci-pull-credentials
+      secret:
+        secretName: ci-pull-credentials
+    - name: manifest-tool-local-pusher
+      secret:
+        secretName: manifest-tool-local-pusher
+    - name: pull-secret
+      secret:
+        secretName: registry-pull-credentials
+    - name: result-aggregator
+      secret:
+        secretName: result-aggregator
+- agent: kubernetes
+  cluster: build07
+  cron: 0 0 * * 6
+  decorate: true
+  decoration_config:
+    sparse_checkout_files:
+    - .ci-operator.yaml
+    - Dockerfile.rhel7
+  extra_refs:
+  - base_ref: release-5.0
+    org: openshift
+    repo: machine-config-operator
+    sparse_checkout_files:
+    - .ci-operator.yaml
+    - Dockerfile.rhel7
+  labels:
+    ci-operator.openshift.io/cloud: aws
+    ci-operator.openshift.io/cloud-cluster-profile: openshift-org-aws
+    ci-operator.openshift.io/variant: periodics
+    ci.openshift.io/generator: prowgen
+    job-release: "5.0"
+    pj-rehearse.openshift.io/can-be-rehearsed: "true"
+  name: periodic-ci-openshift-machine-config-operator-release-5.0-periodics-e2e-aws-mco-single-node-disruptive-techpreview
+  spec:
+    containers:
+    - args:
+      - --gcs-upload-secret=/secrets/gcs/service-account.json
+      - --image-import-pull-secret=/etc/pull-secret/.dockerconfigjson
+      - --lease-server-credentials-file=/etc/boskos/credentials
+      - --report-credentials-file=/etc/report/credentials
+      - --secret-dir=/secrets/ci-pull-credentials
+      - --target=e2e-aws-mco-single-node-disruptive-techpreview
+      - --variant=periodics
+      command:
+      - ci-operator
+      env:
+      - name: HTTP_SERVER_IP
+        valueFrom:
+          fieldRef:
+            fieldPath: status.podIP
+      image: quay-proxy.ci.openshift.org/openshift/ci:ci_ci-operator_latest
+      imagePullPolicy: Always
+      name: ""
+      ports:
+      - containerPort: 8080
+        name: http
+      resources:
+        requests:
+          cpu: 10m
+      volumeMounts:
+      - mountPath: /etc/boskos
+        name: boskos
+        readOnly: true
+      - mountPath: /secrets/ci-pull-credentials
+        name: ci-pull-credentials
+        readOnly: true
+      - mountPath: /secrets/gcs
+        name: gcs-credentials
+        readOnly: true
+      - mountPath: /secrets/manifest-tool
+        name: manifest-tool-local-pusher
+        readOnly: true
+      - mountPath: /etc/pull-secret
+        name: pull-secret
+        readOnly: true
+      - mountPath: /etc/report
+        name: result-aggregator
+        readOnly: true
+    serviceAccountName: ci-operator
+    volumes:
+    - name: boskos
+      secret:
+        items:
+        - key: credentials
+          path: credentials
+        secretName: boskos-credentials
+    - name: ci-pull-credentials
+      secret:
+        secretName: ci-pull-credentials
+    - name: manifest-tool-local-pusher
+      secret:
+        secretName: manifest-tool-local-pusher
+    - name: pull-secret
+      secret:
+        secretName: registry-pull-credentials
+    - name: result-aggregator
+      secret:
+        secretName: result-aggregator
 - agent: kubernetes
   cluster: build03
   decorate: true