We provide a step-by-step tutorial to generate high-quality end-to-end genome annotations in the GENome ANnotation Tutorial (GenAnT). We also provide a series of shell scripts to run each step individually. Lastly, we provide a Snakemake pipeline to generate end-to-end annotations in one line of code + a config file. We are in the mature stages of the peer-review process. This current version has been tested by us and others and functions well. Upon publication we will generate any required updates and fixes before releasing the 1.0.0 version of GenAnT.