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Copy file name to clipboardExpand all lines: _posts/2020-07-26-industrial-koji-fermentation-equipment.md
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@@ -17,17 +17,19 @@ This post will provide a list of commercial and industrial koji fermentation equ
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### Traditional methods
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In traditional koji making methods, cedar vessels are lined with cloth, filled with substrate, and allowed to ferment in a controlled environment called a koji room. The three main types are referred to as the "tray", "box", and "bed" methods - the main difference being the size of the cedar vessel. Craft koji producers and traditional / semi-traditional shops still use these methods today.
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In traditional koji making methods, cedar vessels are lined with cloth, filled with substrate, and allowed to ferment in a controlled environment called a koji room. The three main types are referred to as the "tray", "box", and "table" methods - the main difference being the size of the cedar vessel. Craft koji producers and traditional / semi-traditional shops still use these methods today.
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**Capacity**: 1 - 5 kg per tray, 7.5 - 45kg per box, 100kg per 4m² bed
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**Capacity**: 1 - 5 kg per tray, 7.5 - 45kg per box, 100kg per 4m² table
*Box method in a koji room - from www.nada-ken.com*
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It’s easy to see why these methods won’t scale: labour is expensive. These trays, boxes, and beds have to be loaded and emptied by hand. The koji itself, with no advanced process control, will heat up in the middle of the fermentation process and require mixing by hand to cool.
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Regardless of the method, the general process is the same: steamed rice starts on a *table* (or *toko*) where it's cooled down, inoculated with koji spores, then wrapped up in a pile for the first 18 hours or so for incubation (1). After this incubation period, a process called *mori* is performed in which the rice is transferred to either: 2a) trays, 2b) boxes, or 2c) a 'new' table, which is similar to the first except it's equipped with a wire mesh and adjustable slats.
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It’s easy to see why these methods won’t scale: labour is expensive. These trays, boxes, and tables have to be loaded and emptied by hand. The koji itself, with no advanced process control, will heat up in the middle of the fermentation process and require mixing by hand to cool.
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### Modern equipment
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Interior ventilation is by far the most common. Although surface and non-ventilated methods are described in literature, it seems that the modern equipment market is dominated by interior ventilation methods.
*Unventilated-type closed (left) and with fabric opened (right) - from Fujiwara Techno-Art*
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In these unventilated type machines, the koji sits in a thin layer on a mesh. Two large sheets of plastic fabric are stretched above and below the koji bed. These sheets prevent dust and pathogens from getting onto the koji, but they are still permeable to air and moisture. As the koji heats up, a natural draft forms which allows air to flow through the koji bed. Direct removal of the plastic mesh fabric makes this device easy to clean.
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In these unventilated type machines, the koji sits in a thin layer on a plastic sheet. Two large sheets of plastic fabric are stretched above and below the koji bed. These sheets prevent dust and pathogens from getting onto the koji, but they are still permeable to air and moisture. As the koji heats up, a natural draft forms which allows air to flow through the koji bed. Direct removal of the plastic mesh fabric makes this device easy to clean.
*Drum-type fermenter exterior (top) and interior (bottom) - from iichimiso.com*
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In drum-type fermenters, the koji sits on a mesh mounted approximately halfway between the edge of the drum and its centre axis. This mesh forms the bottom of a pentagonal housing that sits within the drum itself. Within this housing, two static mixers (metal rods with protrusions) extend along the axis of the drum. The drum is normally stationary, and is programmed to turn every so often to facilitate mixing. As it turns, the koji tumbles around and hits these mixers, breaking it up. Drum-type fermenters always have their own air handling unit.
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In drum-type fermenters, the koji sits on a mesh mounted approximately halfway between the edge of the drum and its centre axis. This mesh forms the bottom of a pentagonal housing that sits within the drum itself. Within this housing, two static mixers (metal rods with protrusions) extend along the axis of the drum. The drum is normally stationary, and is programmed to turn every so often to facilitate mixing. As it turns, the koji tumbles around and hits these mixers, breaking it up. Drum-type fermenters always have their own air handling unit. One key advantage of this type of fermenter is that some designs allow you to steam and cool the rice within the drum - saving one additional piece of equipment. Nevertheless, this type seems to have fallen out of favour in recent years.
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This type of fermenter is commonly referred to as a "Tomuzetto" - I have no idea where the etymology comes from. My guess is an portmonteau of "tumble" and "set".
| Machine koji method | 機械製麹法 | Kikai-sei-kiku-hō |
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| Interior ventilation type | 内部通風式 | Naibu-tsufu-shiki |
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| Surface ventilation type | 表面通風式 | Hyomen-tsufu-shiki |
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💡 Note 2: many original equipment manufacturers (OEMs) will come up with their own branded names for equipment. You may see things like "NFT式", "VEX式", etc, but they will always fall into one of the descriptions described here.
Copy file name to clipboardExpand all lines: _posts/2021-06-04-flavourzyme-a-purified-enzyme-mixture-from-a-oryzae.md
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@@ -8,7 +8,7 @@ Flavourzyme™️ is a trademark name given to a mixture of refined proteolytic
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### What is Flavourzyme?
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The modern food processing industry uses proteolytic everywhere: from accelerating cheese aging, modifying breads, as a flavouring (any time you see "hydrolysed vegetable protein"), processed meats, in detergents... the list goes on and on.
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The modern food processing industry uses proteolytic enzymes everywhere: from accelerating cheese aging, modifying breads, as a flavouring (any time you see "hydrolysed vegetable protein"), processed meats, in detergents... the list goes on and on.
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Flavourzyme is a specific mixture of proteolytic enzymes extracted from an *Aspergillus oryzae* liquid culture. What sets it apart from other industrial proteolytic enzymes is that it contains a cocktail of both endo- and exo-peptidases that can efficiently convert proteins into something with more umami, making it useful for applications requiring the development of flavour [1].
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This is why Flavourzyme works well in food applications requiring umami: it contains endopeptidases, which snip peptide bonds in the **middle** of a peptide chain, as well as exopeptidases, which snip peptide bonds at the **terminal ends** of a peptide chain.
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{:height="550" .center}
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*Simplified diagram of the action of endo- and exo-peptidases. Exopeptidases can cut at the terminal peptide bond, one or two bonds away from the terminal peptide bond, or right in the middle of a dipeptide.*
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*Simplified diagram of the action of endo- and exo-peptidases. Exopeptidases can cut at the terminal peptide bond, one or two bonds away from the terminal peptide bond, or right in the middle of a dipeptide.*
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Working together in the correct proportion of endopeptidases and exopeptidases, you end up with an enzyme cocktail that yields plenty of amino acids without accumulating any short, bitter peptides.
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Information here is based off a paper from researchers at the University of Denmark, working at the Novozymes A/S Fermentation Pilot Plant, who wanted to develop a mathematical model for enzyme production with *A. oryzae* in submerged culture [3]. Even though they scrubbed some critical units and sig-figs from their research (for proprietary reasons), it was still enough to piece together details of the process. The process is similar to any other submerged, septic, aerated culture:
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*Process diagram of an aerated bioreactor*
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A seed train is propagated from a pure culture of A. oryzae and then added to an aerated, stirred bioreactor and allowed to grow. The substrate is typically a mixture of food industry by-products such as starch, wheat bran, corn steep powder, and minerals. Once the culture reaches a certain point, the bioreactor is harvested to yield a crude mixture of enzymes, mycelia, and leftover substrate.
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Novozymes A/S won't sell to non-commercial or non-institutional end-users, so I had to import an imitation product from Alibaba. Here's what it looks like:
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{:height="550" .center}
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