Add MutaSeq and ICELL8 cx-based library preparation terms

[Copilot]

New term request for two single-cell library preparation methods. Parent terms evaluated for appropriateness per issue instructions.

Terms Added

MutaSeq (EFO:0920057)

• Parent: Smart-seq2 (EFO:0008931) — more specific than generic "single cell library construction" since MutaSeq is explicitly a Smart-seq2 modification (PMID:33649320)
• Definition: Smart-seq2-based method enabling clonal tracking via targeted amplification of nuclear mutations from cDNA
• Refs: PMID:33649320, PMID:38495438
• Synonym: Mutaseq (related)

ICELL8 cx-based library preparation (EFO:0920058)

• Parent: single cell library construction (EFO:0010183) — appropriate since ICELL8 cx is a platform supporting multiple chemistries
• Definition: Takara Bio platform using 5,184 nano-well chip for high-throughput full-length transcript sequencing with SMART-Seq chemistry
• Refs: PMID:35260714, PMID:36853719
• Synonyms: ICELL8 cx Single-Cell System library preparation (exact), ICELL8 library preparation (related), ICELL8 cx (related)

Structure

<!-- MutaSeq -->
<owl:Class rdf:about="http://www.ebi.ac.uk/efo/EFO_0920057">
    <rdfs:subClassOf rdf:resource="http://www.ebi.ac.uk/efo/EFO_0008931"/>
    <!-- Smart-seq2 parent -->
</owl:Class>

<!-- ICELL8 -->
<owl:Class rdf:about="http://www.ebi.ac.uk/efo/EFO_0920058">
    <rdfs:subClassOf rdf:resource="http://www.ebi.ac.uk/efo/EFO_0010183"/>
    <!-- single cell library construction parent -->
</owl:Class>

Both terms include PMIDs embedded via owl:Axiom annotations on definitions.

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Original prompt

---

This section details on the original issue you should resolve

<issue_title>[NTR] Request for new terms 'Mutaseq' and 'ICELL8 CX-based library preparation'</issue_title>
<issue_description>### Completed template

https://docs.google.com/spreadsheets/d/1mO2EM16nwgNiaaieQFgeeCbyVZMI3NW7vnS4pdnt2Gs/edit?usp=sharing

Any additional comments

No response</issue_description>

<agent_instructions>Please, think if the suggested parents are the most adequate for these terms or there can be a better parent.</agent_instructions>

Comments on the Issue (you are @copilot in this section)

• Fixes [NTR] Request for new terms 'Mutaseq' and 'ICELL8 CX-based library preparation' #2597

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