proliferation-p53-p21

Analysis code for:

Gutu N, Binish N, Keilholz U, Herzel H, Granada AE. p53 and p21 dynamics encode single-cell DNA damage levels, fine-tuning proliferation and shaping population heterogeneity. Communications Biology 2023;6:1196. DOI: 10.1038/s42003-023-05585-5

Contents

Code is organised by figure panels (Fig1, Fig1S, Fig2, Fig2S, Fig3, Fig4, Fig4S). Analysis uses publicly available datasets (PMID: 32049575 and PMID: 30057196) to characterise how DNA damage levels are quantitatively translated into p53 and p21 signal parameters and their relationship to single-cell proliferation heterogeneity.

Requirements

• Python 3.8 or later
• numpy, scipy, pandas, matplotlib, seaborn
• pyBOAT for time-series analysis of signalling traces

License

MIT — see LICENSE. This code was developed by members and collaborators of the Granada Lab research group. For authorship and correspondence, please refer to the publication above.