# SRRF.py

A Python script to automatically perform SRRF analysis (https://github.com/HenriquesLab) with several different settings directly from the FIJI scripting window.

#How to use it:

• Open the script in the FIJI/ImageJ.
• Before running the Macro, update sites required are: sites.imagej.net/NanoJ/; sites.imagej.net/NanoJ-SQUIRREL/; sites.imagej.net/NanoJ-SRRF/; biop.epfl.ch/Fiji-Update/;
• Place all the .tif stacks in a folder
• Run the script
• It will ask you for the folder with the .tif stacks
• It will ask you for the folder where to place the results

# SRRF-macro This Macro was written by Prabhu Prasad Swain and we published it in (https://doi.org/10.1016/j.jsb.2023.107982)

Make sure to run ImageJ V1.53v11 (if you don't find it, go to Help>Update ImageJ > Choose the "daily build")

//Before running the Macro, update sites required are: // https://sites.imagej.net/BIG-EPFL/ // https://sites.imagej.net/NanoJ/ // https://sites.imagej.net/NanoJ-SQUIRREL/ // https://sites.imagej.net/NanoJ-SRRF/