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v2.1.0
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released this
01 Jul 21:32
Cell-calling is done for each sub-library separately, improving performance for large runs
Changes to CellFinder to align more closely with EmptyDrops
Cell-barcodes far below the median unique transcript count (medianFraction) are never called cells
Beads exposed to a large ambient RNA signal are filtered by default
Ultima .cram input supported without fixed filename pattern
Short reads after adapter and Poly-A trimming are not longer reported in the library QC reports
These reads are included in "Total Sample Reads" in the sample reports
Saturation is always calculated on all transcriptome reads
Unique and multimappers and both genomes for barnyard samples
Barcode naming changes
The barcode from the RT plate is called RT and no longer pbc barcode
Bead barcode blocks are 01-96, instead of 1A-12H
The workflow now checks minimal compute resource requirements after start-up
Updated example samplesheet.csv files
The index orientation needed for NextSeq 2000 and NovaSeq X is now the default, rather than _revComp
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