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2.1.0

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released this 01 Jul 21:32
  • Cell-calling is done for each sub-library separately, improving performance for large runs
  • Changes to CellFinder to align more closely with EmptyDrops
    • Cell-barcodes far below the median unique transcript count (medianFraction) are never called cells
  • Beads exposed to a large ambient RNA signal are filtered by default
  • Ultima .cram input supported without fixed filename pattern
  • Short reads after adapter and Poly-A trimming are not longer reported in the library QC reports
    • These reads are included in "Total Sample Reads" in the sample reports
  • Saturation is always calculated on all transcriptome reads
    • Unique and multimappers and both genomes for barnyard samples
  • Barcode naming changes
    • The barcode from the RT plate is called RT and no longer pbc barcode
    • Bead barcode blocks are 01-96, instead of 1A-12H
  • The workflow now checks minimal compute resource requirements after start-up
  • Updated example samplesheet.csv files
    • The index orientation needed for NextSeq 2000 and NovaSeq X is now the default, rather than _revComp