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Zoospore Tracking

Experiment Protocol

Light intensity levels are changed many times during a single experimental run, with phases (step up, step down, and adaptation). Numbers in brackets are codes for the light level. image

Possible metrics

image

Implemented metrics

TO DO: Update the metrics below to show the latest implementation

  • Frequency of direction change. Angular velocity vector, with threshold for turn angle directional change.
  • Mean Squared Displacement (MSD).
  • Straight-line velocity (VSL). The time-average velocity of the zoospore object along a straight line between its first detected position and its last position.
  • Curvilinear velocity (VCL). The time-average velocity of the object along its actual trajectory.
  • Directionality ratio. A measure of how straight the overall path of movement is, defined as the ratio of the net displacement to the total path length.
  • Area covered. The spatial extent of the area explored by the organism.
  • Average speed. The mean speed of a particle of the entire trajectory.

Execute image processing

TO DO: Needs update for Nextflow pipeline

Inspect output data

Run the script data_app.py and open the resulting local URL in the browser.

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