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Background

The three tumultuous years of my master's program are coming to an end, and the result of my Ph.D application remains unknown.

Here, I would like to organize the next-generation sequencing(NGS) data analysis I have done during my master's phase, mainly focusing on learning materials and scripts.

The construction of this repository is based on the bioinformatics notes I initially learned, which were written by postdoc researcher Dr.Fajin Li.

I am extremely fortunate to have met him during my master's studies. Under his guidance, I began my journey into bioinformatics, not only resolving doubts in my research topics but also gradually understanding how to think critically in scientific research. More importantly, I have learned valuable qualities of a outstanding researcher from him.

Design

So far, the data analysis I have been involved in has primarily focused on translation regulation using ribosome profiling technology. Additionally, I have also completed data analysis for RNA-seq, scRNA-seq, and PAR-CLIP seq, but these analyses were not particularly in-depth.

Projects

  1. Ribo-seq data analysis
  2. RNA-seq data analysis
  3. PAR-CLIP seq data analysis
  4. scRNA-seq data analysis (10x Genomics)

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My own studied materials and scripts (only update in my spare time)

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